REL
2019-12-13
2020-01-31
2020-02-07
CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations)
0000-0003-1909-5751
Melody
Grace
Campbell
UCSF
600 16th Street, Room S474
San Francisco
CA
United States
94143
0000-0001-9535-0369
Yifan
Cheng
Department of Biochemistry and Biophysics, University of California, San Francisco
600 16th Street, Room S472D
San Francisco
CA
United States
94143
Campbell MG
Cheng Y
882.1
10.6019/EMPIAR-10345
EMDB
EMD-20795
Campbell MG
Cormier A
Ito S
Seed RI
Bondesson AJ
Lou J
Marks JD
Baron J
Cheng Y
Nishimura SL
Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β
Cell
Cell
2020
English
10.1016/j.cell.2019.12.030
31955848
Unaligned 60-frame movies of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid
/data/Movies_18jam15a
micrographs - multiframe
TIFF
TIFF
1644
60
UNSIGNED BYTE
7676
0.6725
7420
0.6725
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 12 second movies contain 60 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~55 e/Å^2. The gain reference anda file containing coordinates for a single dead column.
data/Movies_18jam15a/18ja****_****.tif
data/Stack_18jam15a/Micrographs/18ja*_ali_DW_extract.star
data/Stack_18jam15a/Micrographs
Dose-weighted aligned micrographs of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid
/data/Micrographs_18jam15a
micrographs - single frame
MRC
MRC
1644
1
32 BIT FLOAT
3838
1.345
3710
1.345
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~55 e/Å^2.
data/Micrographs_18jam15a/18ja*_ali_DW.mrc
data/Stack_18jam15a/Micrographs/18ja*_ali_DW_extract.star
data/Stack_18jam15a/Micrographs
Dose-weighted aligned particle stacks of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid
/data/Stack_18jam15a
picked particles - single frame - processed
MRCS
MRCS
1642
1
32 BIT FLOAT
300
1.345
300
1.345
αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): Stacks of 84,266 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after 2D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345.
data/Micrographs_18jam15a/18ja****_****_ali_DW.mrc
data/Stack_18jam15a/Micrographs/18ja****_****_ali_DW_extract.star
data/Stack_18jam15a/Micrographs