DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/17mar28c/protomo_alignments/tiltseries0008/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/17mar28c/protomo_alignments/tiltseries0008/defocus_estimation/series0008_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/17mar28c/protomo_alignments/tiltseries0008/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 25200.0, 37800.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 54.0 27000.0 28000.0 35500.0 1 52.0 28000.0 37500.0 35500.0 2 50.0 27000.0 37500.0 35000.0 3 48.0 27000.0 37500.0 35500.0 4 46.0 26500.0 37500.0 35000.0 5 44.0 25500.0 37500.0 34500.0 6 42.0 37500.0 37000.0 34000.0 7 40.0 29000.0 37000.0 34500.0 8 38.0 37500.0 36500.0 33500.0 9 36.0 37500.0 36500.0 34000.0 10 34.0 37500.0 36500.0 34500.0 11 32.0 37500.0 36000.0 34000.0 12 30.0 37000.0 35500.0 33500.0 13 28.0 36500.0 34500.0 32500.0 14 26.0 36000.0 34000.0 33000.0 15 24.0 35000.0 33500.0 31500.0 16 22.0 34500.0 33500.0 32000.0 17 20.0 34000.0 33000.0 31500.0 18 18.0 34000.0 32500.0 31500.0 19 16.0 33500.0 32500.0 31500.0 20 14.0 33500.0 32000.0 32000.0 21 12.0 33000.0 32000.0 31500.0 22 10.0 33000.0 32500.0 31500.0 23 8.0 32500.0 32000.0 31500.0 24 6.0 32500.0 31500.0 31500.0 25 4.0 32000.0 32000.0 31000.0 26 2.0 32000.0 32000.0 31500.0 27 0.0 32000.0 32000.0 31500.0 28 -0.0 31500.0 31500.0 31000.0 29 -2.0 31000.0 31000.0 31500.0 30 -4.0 31000.0 31000.0 30500.0 31 -6.0 31000.0 31000.0 31000.0 32 -8.0 30500.0 31000.0 31500.0 33 -10.0 31000.0 31000.0 31500.0 34 -12.0 31000.0 32000.0 32000.0 35 -14.0 32000.0 32000.0 32500.0 36 -16.0 31500.0 32500.0 33500.0 37 -18.0 32000.0 32500.0 33000.0 38 -20.0 31500.0 32000.0 33000.0 39 -22.0 31500.0 32000.0 33000.0 40 -24.0 31000.0 31500.0 33000.0 41 -26.0 30500.0 31500.0 33000.0 42 -28.0 30500.0 31000.0 33000.0 43 -30.0 30000.0 31500.0 32500.0 44 -32.0 30000.0 31500.0 33000.0 45 -34.0 29500.0 31500.0 33500.0 46 -36.0 30000.0 31500.0 33500.0 47 -38.0 30000.0 31500.0 33500.0 48 -40.0 30500.0 31500.0 34000.0 49 -42.0 29500.0 32000.0 34000.0 50 -44.0 30500.0 33000.0 34500.0 51 -46.0 30500.0 32500.0 35500.0 52 -48.0 30500.0 32000.0 35000.0 53 -50.0 28500.0 31500.0 34500.0 54 -52.0 27500.0 30500.0 33500.0 55 -54.0 26500.0 30000.0 33000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)