REL
2018-10-04
2019-04-18
2019-06-07
CryoET of cis-mutated mouse protocadherin gamma B6 on membranes
Lawrence
Shapiro
Zuckerman Mind, Brain and Behavior Institute, Columbia University
3227 Broadway
New York
New York
United States
10027
Lawrence
Shapiro
Zuckerman Mind, Brain and Behavior Institute, Columbia University
3227 Broadway
New York
New York
United States
10027
Brasch J
Bepler T
Berger B
Maniatis T
Potter CS
Carragher B
Honig B
Shapiro L
379.7
10.6019/EMPIAR-10236
EMDB
EMD-9200
Brasch J
Goodman KM
Noble AJ
Mannepalli S
Bahna F
Rapp M
Dandey VP
Bepler T
Berger B
Maniatis T
Potter CS
Carragher B
Honig B
Shapiro L
Visualization of clustered protocadherin neuronal self-recognition complexes
Nature
Nature
United States
7755
569
280
283
2019
English
10.1038/s41586-019-1089-3
30971825
Includes raw frames, aligned micrographs using MotionCor2, naively summed micrographs, tilt-series for exemplar areas, aligned tilt-series for exemplar areas, and tomograms.
K2 tilt-series frames
/data/frames
micrographs - multiframe
MRCS
MRC
58
7
UNSIGNED 16 BIT INTEGER
3710
1.84
3838
1.84
~8 e-/px/s dose rate on the camera
Gain and dark references included
Whole-frame aligned tilt images along with all other magnification images from the collection
/data/leginon
micrographs - single frame
MRC
MRC
58
1
32 BIT FLOAT
3710
1.84
3838
1.84
Whole-frame aligned tilt images using Motioncor2.
Appion-Protomo tilt-series alignments
/data/protomo_alignments
tilt series
MRC
MRC
5
1
32 BIT FLOAT
3710
1.84
3838
1.84
Includes a full tilt-series alignment run for each tilt-series.
To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
The export/ directory contains SerialEM-formatted unaligned tilt-series stacks, mdoc files, and tilt files. This allows for easy upload to Appion-Protomo.