DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/18mar15h/protomo_alignments/tiltseries0006/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/18mar15h/protomo_alignments/tiltseries0006/defocus_estimation/series0006_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/18mar15h/protomo_alignments/tiltseries0006/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.3 Angstroms/Pixel: 1.33 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 5.3 dFMin[A], dFMax[A], FStep: 40000.0, 60000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 45.0 41500.0 45500.0 41500.0 1 42.0 43500.0 60000.0 45000.0 2 39.0 47500.0 40500.0 46000.0 3 36.0 45000.0 51000.0 47500.0 4 33.0 41000.0 41500.0 41500.0 5 30.0 45000.0 40500.0 40500.0 6 27.0 42500.0 40500.0 40500.0 7 24.0 42000.0 46000.0 59500.0 8 21.0 60000.0 60000.0 60000.0 9 18.0 60000.0 60000.0 60000.0 10 15.0 40000.0 59500.0 59500.0 11 12.0 59000.0 59000.0 59000.0 12 9.0 58000.0 58000.0 57000.0 13 6.0 57000.0 56500.0 56500.0 14 3.0 55500.0 55500.0 55500.0 15 0.1 54500.0 55500.0 42000.0 16 -0.1 53500.0 55000.0 55000.0 17 -3.0 53500.0 52500.0 53500.0 18 -6.0 51500.0 51500.0 51500.0 19 -9.0 50000.0 51500.0 51000.0 20 -12.0 49500.0 50000.0 50000.0 21 -15.0 47500.0 48500.0 48500.0 22 -18.0 46500.0 47500.0 49500.0 23 -21.0 45500.0 44000.0 47500.0 24 -24.0 45000.0 46500.0 46000.0 25 -27.0 43500.0 46000.0 46500.0 26 -30.0 43500.0 44500.0 45500.0 27 -33.0 41000.0 42500.0 44000.0 28 -36.0 41000.0 42500.0 44000.0 29 -39.0 40000.0 41000.0 42500.0 30 -42.0 40000.0 40000.0 41000.0 31 -45.0 50000.0 40000.0 40000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 70.154u 0.824s 1:11.17 99.7% 0+0k 0+0io 0pf+0w