DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/16dec22b/protomo_alignments/tiltseries0005/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/16dec22b/protomo_alignments/tiltseries0005/defocus_estimation/series0005_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/16dec22b/protomo_alignments/tiltseries0005/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 45.0 37000.0 40500.0 44500.0 1 42.0 47500.0 48000.0 35500.0 2 39.0 36000.0 32500.0 45500.0 3 36.0 32500.0 47000.0 33000.0 4 33.0 32000.0 37000.0 35000.0 5 30.0 37500.0 37000.0 37000.0 6 27.0 44500.0 46500.0 34500.0 7 24.0 47500.0 33500.0 42000.0 8 21.0 42500.0 47500.0 37500.0 9 18.0 44500.0 32000.0 38000.0 10 15.0 39500.0 40500.0 33000.0 11 12.0 46000.0 47500.0 48000.0 12 9.0 46500.0 32000.0 47500.0 13 6.0 44500.0 47000.0 32000.0 14 3.0 45500.0 33000.0 46000.0 15 0.1 46000.0 47000.0 45000.0 16 -0.1 45500.0 47000.0 46500.0 17 -3.0 46000.0 45500.0 45500.0 18 -6.0 45000.0 44500.0 44000.0 19 -9.0 45000.0 44500.0 43500.0 20 -12.0 44500.0 48000.0 44000.0 21 -15.0 37500.0 43000.0 42500.0 22 -18.0 44000.0 37000.0 34500.0 23 -21.0 41000.0 37000.0 38000.0 24 -24.0 42500.0 38500.0 35000.0 25 -27.0 42500.0 38000.0 36500.0 26 -30.0 43500.0 38500.0 38000.0 27 -33.0 32000.0 38000.0 32500.0 28 -36.0 44500.0 37000.0 41000.0 29 -39.0 35000.0 48000.0 36000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)