DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0006/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0006/defocus_estimation/series0006_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0006/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 43400.0, 65100.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -0.1 43000.0 43000.0 43000.0 1 -3.0 52000.0 43000.0 55000.0 2 -6.0 43000.0 43500.0 43000.0 3 -9.0 63000.0 50000.0 51500.0 4 -12.0 52000.0 50000.0 44000.0 5 -15.0 44000.0 58500.0 44000.0 6 -18.0 55500.0 57000.0 46000.0 7 -21.0 54000.0 44000.0 43500.0 8 -24.0 59000.0 51000.0 44000.0 9 -27.0 46000.0 60500.0 55500.0 10 -30.0 52500.0 63500.0 50000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)