DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0006/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0006/defocus_estimation/series0006_tilts.txt Output diagnostic file name: /gpfs/appion/anoble/16dec29d/protomo_alignments/tiltseries0006/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 4.3, 6.51, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 0.1 0.0 0.0 0.0 1 3.0 0.0 0.0 0.0 2 6.0 0.0 0.0 0.0 3 9.0 0.0 0.0 0.0 4 12.0 0.0 0.0 0.0 5 15.0 0.0 0.0 0.0 6 18.0 0.0 0.0 0.0 7 21.0 0.0 0.0 0.0 8 24.0 0.0 0.0 0.0 9 27.0 0.0 0.0 0.0 10 30.0 0.0 0.0 0.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)