- FEI Titan Krios microscope operated at 300 kV. - Defocus range -800 to -1700 nm (underfocus). - Magnification 37,000x, 50 um C2 aperture, 70 um OL aperture, spot size 6, 1.4 um beam diameter, zero-loss imaging with 20 eV slit. - K2 Summit in counting mode. - Pixel size 1.35 Å. - Total dose 30 e-/ Å2. - Dose rate on the detector 9.1 e-/pixel/s. - Exposure time 6 s. - 12 frames, 0.5 s each.