- FEI Titan Krios microscope operated at 300 kV.
- Volta phase plate.
- Target defocus -20 nm (underfocus).
- Magnification 37,000x, 50 um C2 aperture, spot size 6, 1.4 um beam diameter, zero-loss imaging with 20 eV slit.
- K2 Summit in counting mode.
- Pixel size 1.35 Å.
- Total dose 30 e-/ Å2.
- Dose rate on the detector 9.1 e-/pixel/s.
- Exposure time 6 s.
- 12 frames, 0.5 s each.