CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/479.mrc Output diagnostic file name Micrographs/479.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/479.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1790.000 7750.000 Mean, RMS : 4490.525 228.1011 TITLE 1: emi2mrc: 479.emi Mon Jan 20 12:46:34 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X -426.364, Y 497.117, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01693 10800.00 8000.00 0.00 -0.01688 11000.00 8000.00 0.00 -0.01644 11200.00 8000.00 0.00 -0.01579 11400.00 8000.00 0.00 -0.01493 11600.00 8000.00 0.00 -0.01392 11800.00 8000.00 0.00 -0.01285 12000.00 8000.00 0.00 -0.01182 12200.00 8000.00 0.00 -0.01084 12400.00 8000.00 0.00 -0.00985 12600.00 8000.00 0.00 -0.00877 12800.00 8000.00 0.00 -0.00751 13000.00 8000.00 0.00 -0.00610 13200.00 8000.00 0.00 -0.00462 13400.00 8000.00 0.00 -0.00318 13600.00 8000.00 0.00 -0.00184 13800.00 8000.00 0.00 -0.00057 14000.00 8000.00 0.00 0.00071 14200.00 8000.00 0.00 0.00210 14400.00 8000.00 0.00 0.00366 14600.00 8000.00 0.00 0.00538 14800.00 8000.00 0.00 0.00721 15000.00 8000.00 0.00 0.00913 15200.00 8000.00 0.00 0.01112 15400.00 8000.00 0.00 0.01315 15600.00 8000.00 0.00 0.01521 15800.00 8000.00 0.00 0.01725 16000.00 8000.00 0.00 0.01925 16200.00 8000.00 0.00 0.02125 16400.00 8000.00 0.00 0.02332 16600.00 8000.00 0.00 0.02553 16800.00 8000.00 0.00 0.02790 17000.00 8000.00 0.00 0.03041 17200.00 8000.00 0.00 0.03296 17400.00 8000.00 0.00 0.03552 17600.00 8000.00 0.00 0.03808 17800.00 8000.00 0.00 0.04071 18000.00 8000.00 0.00 0.04345 18200.00 8000.00 0.00 0.04630 18400.00 8000.00 0.00 0.04923 18600.00 8000.00 0.00 0.05215 18800.00 8000.00 0.00 0.05506 19000.00 8000.00 0.00 0.05797 19200.00 8000.00 0.00 0.06090 19400.00 8000.00 0.00 0.06380 19600.00 8000.00 0.00 0.06657 19800.00 8000.00 0.00 0.06904 20000.00 8000.00 0.00 0.07111 20200.00 8000.00 0.00 0.07270 20400.00 8000.00 0.00 0.07384 20600.00 8000.00 0.00 0.07460 20800.00 8000.00 0.00 0.07508 21000.00 8000.00 0.00 0.07530 20600.00 8200.00 0.00 0.07546 20800.00 8200.00 0.00 0.07592 21000.00 8200.00 0.00 0.07613 20600.00 8400.00 0.00 0.07635 20800.00 8400.00 0.00 0.07680 21000.00 8400.00 0.00 0.07700 20600.00 8600.00 0.00 0.07740 20800.00 8600.00 0.00 0.07784 21000.00 8600.00 0.00 0.07803 20600.00 8800.00 0.00 0.07854 20800.00 8800.00 0.00 0.07897 21000.00 8800.00 0.00 0.07915 20600.00 9000.00 0.00 0.07963 20800.00 9000.00 0.00 0.08005 21000.00 9000.00 0.00 0.08023 20600.00 9200.00 0.00 0.08064 20800.00 9200.00 0.00 0.08105 21000.00 9200.00 0.00 0.08123 20600.00 9400.00 0.00 0.08156 20800.00 9400.00 0.00 0.08197 21000.00 9400.00 0.00 0.08215 20600.00 9600.00 0.00 0.08248 20800.00 9600.00 0.00 0.08290 21000.00 9600.00 0.00 0.08309 20600.00 9800.00 0.00 0.08358 20800.00 9800.00 0.00 0.08402 21000.00 9800.00 0.00 0.08422 21200.00 9800.00 0.00 0.08423 20400.00 10000.00 0.00 0.08424 20600.00 10000.00 0.00 0.08497 20800.00 10000.00 0.00 0.08542 21000.00 10000.00 0.00 0.08564 21200.00 10000.00 0.00 0.08565 20400.00 10200.00 0.00 0.08578 20600.00 10200.00 0.00 0.08653 20800.00 10200.00 0.00 0.08699 21000.00 10200.00 0.00 0.08722 20400.00 10400.00 0.00 0.08740 20600.00 10400.00 0.00 0.08815 20800.00 10400.00 0.00 0.08862 21000.00 10400.00 0.00 0.08883 20400.00 10600.00 0.00 0.08901 20600.00 10600.00 0.00 0.08976 20800.00 10600.00 0.00 0.09021 21000.00 10600.00 0.00 0.09040 20400.00 10800.00 0.00 0.09057 20600.00 10800.00 0.00 0.09130 20800.00 10800.00 0.00 0.09173 21000.00 10800.00 0.00 0.09187 20400.00 11000.00 0.00 0.09217 20600.00 11000.00 0.00 0.09287 20800.00 11000.00 0.00 0.09326 21000.00 11000.00 0.00 0.09337 20400.00 11200.00 0.00 0.09393 20600.00 11200.00 0.00 0.09460 20800.00 11200.00 0.00 0.09496 21000.00 11200.00 0.00 0.09504 20400.00 11400.00 0.00 0.09585 20600.00 11400.00 0.00 0.09650 20800.00 11400.00 0.00 0.09682 21000.00 11400.00 0.00 0.09686 20400.00 11600.00 0.00 0.09789 20600.00 11600.00 0.00 0.09850 20800.00 11600.00 0.00 0.09879 21000.00 11600.00 0.00 0.09879 20200.00 11800.00 0.00 0.09897 20400.00 11800.00 0.00 0.09999 20600.00 11800.00 0.00 0.10056 20800.00 11800.00 0.00 0.10081 20200.00 12000.00 0.00 0.10110 20400.00 12000.00 0.00 0.10208 20600.00 12000.00 0.00 0.10261 20800.00 12000.00 0.00 0.10283 20200.00 12200.00 0.00 0.10322 20400.00 12200.00 0.00 0.10416 20600.00 12200.00 0.00 0.10465 20800.00 12200.00 0.00 0.10483 20200.00 12400.00 0.00 0.10539 20400.00 12400.00 0.00 0.10629 20600.00 12400.00 0.00 0.10675 20800.00 12400.00 0.00 0.10690 20200.00 12600.00 0.00 0.10755 20400.00 12600.00 0.00 0.10843 20600.00 12600.00 0.00 0.10886 20800.00 12600.00 0.00 0.10898 20200.00 12800.00 0.00 0.10964 20400.00 12800.00 0.00 0.11050 20600.00 12800.00 0.00 0.11092 20800.00 12800.00 0.00 0.11103 20200.00 13000.00 0.00 0.11172 20400.00 13000.00 0.00 0.11258 20600.00 13000.00 0.00 0.11299 20800.00 13000.00 0.00 0.11309 20200.00 13200.00 0.00 0.11388 20400.00 13200.00 0.00 0.11473 20600.00 13200.00 0.00 0.11515 20800.00 13200.00 0.00 0.11524 20200.00 13400.00 0.00 0.11622 20400.00 13400.00 0.00 0.11707 20600.00 13400.00 0.00 0.11748 20800.00 13400.00 0.00 0.11756 20200.00 13600.00 0.00 0.11891 20400.00 13600.00 0.00 0.11976 20600.00 13600.00 0.00 0.12014 20800.00 13600.00 0.00 0.12019 20000.00 13800.00 0.00 0.12047 20200.00 13800.00 0.00 0.12193 20400.00 13800.00 0.00 0.12275 20600.00 13800.00 0.00 0.12309 20000.00 14000.00 0.00 0.12359 20200.00 14000.00 0.00 0.12501 20400.00 14000.00 0.00 0.12578 20600.00 14000.00 0.00 0.12605 20000.00 14200.00 0.00 0.12659 20200.00 14200.00 0.00 0.12796 20400.00 14200.00 0.00 0.12865 20600.00 14200.00 0.00 0.12884 20000.00 14400.00 0.00 0.12940 20200.00 14400.00 0.00 0.13069 20400.00 14400.00 0.00 0.13131 20600.00 14400.00 0.00 0.13144 20000.00 14600.00 0.00 0.13204 20200.00 14600.00 0.00 0.13327 20400.00 14600.00 0.00 0.13383 20600.00 14600.00 0.00 0.13391 20000.00 14800.00 0.00 0.13476 20200.00 14800.00 0.00 0.13595 20400.00 14800.00 0.00 0.13646 20600.00 14800.00 0.00 0.13650 20000.00 15000.00 0.00 0.13784 20200.00 15000.00 0.00 0.13898 20400.00 15000.00 0.00 0.13945 20000.00 15200.00 0.00 0.14118 20200.00 15200.00 0.00 0.14229 20400.00 15200.00 0.00 0.14272 20000.00 15400.00 0.00 0.14460 20200.00 15400.00 0.00 0.14567 20400.00 15400.00 0.00 0.14606 19800.00 15600.00 0.00 0.14614 20000.00 15600.00 0.00 0.14800 20200.00 15600.00 0.00 0.14903 20400.00 15600.00 0.00 0.14939 19800.00 15800.00 0.00 0.14944 20000.00 15800.00 0.00 0.15128 20200.00 15800.00 0.00 0.15227 20400.00 15800.00 0.00 0.15259 19800.00 16000.00 0.00 0.15269 20000.00 16000.00 0.00 0.15450 20200.00 16000.00 0.00 0.15546 20400.00 16000.00 0.00 0.15574 19800.00 16200.00 0.00 0.15617 20000.00 16200.00 0.00 0.15795 20200.00 16200.00 0.00 0.15888 20400.00 16200.00 0.00 0.15912 19800.00 16400.00 0.00 0.15992 20000.00 16400.00 0.00 0.16169 20200.00 16400.00 0.00 0.16257 20400.00 16400.00 0.00 0.16276 19800.00 16600.00 0.00 0.16375 20000.00 16600.00 0.00 0.16548 20200.00 16600.00 0.00 0.16633 20400.00 16600.00 0.00 0.16645 19800.00 16800.00 0.00 0.16752 20000.00 16800.00 0.00 0.16924 20200.00 16800.00 0.00 0.17003 20400.00 16800.00 0.00 0.17008 19800.00 17000.00 0.00 0.17121 20000.00 17000.00 0.00 0.17289 20200.00 17000.00 0.00 0.17363 19800.00 17200.00 0.00 0.17483 20000.00 17200.00 0.00 0.17648 20200.00 17200.00 0.00 0.17714 19800.00 17400.00 0.00 0.17872 20000.00 17400.00 0.00 0.18030 20200.00 17400.00 0.00 0.18085 19800.00 17600.00 0.00 0.18307 20000.00 17600.00 0.00 0.18454 20200.00 17600.00 0.00 0.18494 19600.00 17800.00 0.00 0.18495 19800.00 17800.00 0.00 0.18763 20000.00 17800.00 0.00 0.18895 20200.00 17800.00 0.00 0.18916 19600.00 18000.00 0.00 0.18957 19800.00 18000.00 0.00 0.19215 20000.00 18000.00 0.00 0.19329 19600.00 18200.00 0.00 0.19407 19800.00 18200.00 0.00 0.19652 20000.00 18200.00 0.00 0.19746 19600.00 18400.00 0.00 0.19840 19800.00 18400.00 0.00 0.20074 20000.00 18400.00 0.00 0.20146 19600.00 18600.00 0.00 0.20299 19800.00 18600.00 0.00 0.20517 20000.00 18600.00 0.00 0.20565 19600.00 18800.00 0.00 0.20834 19800.00 18800.00 0.00 0.21026 20000.00 18800.00 0.00 0.21036 19400.00 19000.00 0.00 0.21065 19600.00 19000.00 0.00 0.21410 19800.00 19000.00 0.00 0.21560 19400.00 19200.00 0.00 0.21631 19600.00 19200.00 0.00 0.21935 19800.00 19200.00 0.00 0.22025 19400.00 19400.00 0.00 0.22070 19600.00 19400.00 0.00 0.22312 19800.00 19400.00 0.00 0.22325 19600.00 19600.00 0.00 0.22435 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19662.74 19546.18 -21.35 0.22451 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/479.ctf NX, NY, NZ: 360 360 1 MODE : real