CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/473.mrc Output diagnostic file name Micrographs/473.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/473.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2464.000 8862.000 Mean, RMS : 4428.751 223.0456 TITLE 1: emi2mrc: 473.emi Mon Jan 20 12:42:31 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X -431.089, Y 494.870, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01621 8200.00 8000.00 0.00 -0.01605 8400.00 8000.00 0.00 -0.01558 8600.00 8000.00 0.00 -0.01495 8800.00 8000.00 0.00 -0.01431 9000.00 8000.00 0.00 -0.01373 9200.00 8000.00 0.00 -0.01323 9400.00 8000.00 0.00 -0.01280 9600.00 8000.00 0.00 -0.01237 9800.00 8000.00 0.00 -0.01190 10000.00 8000.00 0.00 -0.01136 10200.00 8000.00 0.00 -0.01075 10400.00 8000.00 0.00 -0.01007 10600.00 8000.00 0.00 -0.00939 10800.00 8000.00 0.00 -0.00877 11000.00 8000.00 0.00 -0.00823 11200.00 8000.00 0.00 -0.00771 11400.00 8000.00 0.00 -0.00710 11600.00 8000.00 0.00 -0.00629 11800.00 8000.00 0.00 -0.00526 12000.00 8000.00 0.00 -0.00407 12200.00 8000.00 0.00 -0.00289 12400.00 8000.00 0.00 -0.00183 12600.00 8000.00 0.00 -0.00089 12800.00 8000.00 0.00 0.00004 13000.00 8000.00 0.00 0.00115 13200.00 8000.00 0.00 0.00255 13400.00 8000.00 0.00 0.00420 13600.00 8000.00 0.00 0.00597 13800.00 8000.00 0.00 0.00769 14000.00 8000.00 0.00 0.00928 14200.00 8000.00 0.00 0.01076 14400.00 8000.00 0.00 0.01229 14600.00 8000.00 0.00 0.01396 14800.00 8000.00 0.00 0.01582 15000.00 8000.00 0.00 0.01782 15200.00 8000.00 0.00 0.01988 15400.00 8000.00 0.00 0.02194 15600.00 8000.00 0.00 0.02402 15800.00 8000.00 0.00 0.02620 16000.00 8000.00 0.00 0.02857 16200.00 8000.00 0.00 0.03115 16400.00 8000.00 0.00 0.03392 16600.00 8000.00 0.00 0.03680 16800.00 8000.00 0.00 0.03972 17000.00 8000.00 0.00 0.04266 17200.00 8000.00 0.00 0.04559 17400.00 8000.00 0.00 0.04853 17600.00 8000.00 0.00 0.05147 17800.00 8000.00 0.00 0.05440 18000.00 8000.00 0.00 0.05732 18200.00 8000.00 0.00 0.06030 18400.00 8000.00 0.00 0.06342 18600.00 8000.00 0.00 0.06674 18800.00 8000.00 0.00 0.07029 19000.00 8000.00 0.00 0.07391 19200.00 8000.00 0.00 0.07731 19400.00 8000.00 0.00 0.08017 19600.00 8000.00 0.00 0.08225 19800.00 8000.00 0.00 0.08348 20000.00 8000.00 0.00 0.08402 20200.00 8000.00 0.00 0.08416 19800.00 8200.00 0.00 0.08428 20000.00 8200.00 0.00 0.08487 20200.00 8200.00 0.00 0.08505 20400.00 8200.00 0.00 0.08506 19800.00 8400.00 0.00 0.08520 20000.00 8400.00 0.00 0.08583 20200.00 8400.00 0.00 0.08605 20400.00 8400.00 0.00 0.08609 20600.00 8400.00 0.00 0.08611 20800.00 8400.00 0.00 0.08611 19800.00 8600.00 0.00 0.08637 20000.00 8600.00 0.00 0.08703 20200.00 8600.00 0.00 0.08728 20400.00 8600.00 0.00 0.08734 20600.00 8600.00 0.00 0.08737 19800.00 8800.00 0.00 0.08774 20000.00 8800.00 0.00 0.08842 20200.00 8800.00 0.00 0.08868 20400.00 8800.00 0.00 0.08874 20600.00 8800.00 0.00 0.08876 19800.00 9000.00 0.00 0.08916 20000.00 9000.00 0.00 0.08984 20200.00 9000.00 0.00 0.09009 20400.00 9000.00 0.00 0.09015 19800.00 9200.00 0.00 0.09054 20000.00 9200.00 0.00 0.09122 20200.00 9200.00 0.00 0.09146 20400.00 9200.00 0.00 0.09150 19800.00 9400.00 0.00 0.09183 20000.00 9400.00 0.00 0.09250 20200.00 9400.00 0.00 0.09274 20400.00 9400.00 0.00 0.09275 19800.00 9600.00 0.00 0.09306 20000.00 9600.00 0.00 0.09372 20200.00 9600.00 0.00 0.09395 19800.00 9800.00 0.00 0.09438 20000.00 9800.00 0.00 0.09504 20200.00 9800.00 0.00 0.09525 19800.00 10000.00 0.00 0.09588 20000.00 10000.00 0.00 0.09654 20200.00 10000.00 0.00 0.09672 19800.00 10200.00 0.00 0.09747 20000.00 10200.00 0.00 0.09810 20200.00 10200.00 0.00 0.09825 19800.00 10400.00 0.00 0.09906 20000.00 10400.00 0.00 0.09966 20200.00 10400.00 0.00 0.09977 19800.00 10600.00 0.00 0.10060 20000.00 10600.00 0.00 0.10117 20200.00 10600.00 0.00 0.10125 19800.00 10800.00 0.00 0.10209 20000.00 10800.00 0.00 0.10263 20200.00 10800.00 0.00 0.10269 19800.00 11000.00 0.00 0.10367 20000.00 11000.00 0.00 0.10419 20200.00 11000.00 0.00 0.10423 19800.00 11200.00 0.00 0.10550 20000.00 11200.00 0.00 0.10601 20200.00 11200.00 0.00 0.10603 19600.00 11400.00 0.00 0.10623 19800.00 11400.00 0.00 0.10754 20000.00 11400.00 0.00 0.10805 20200.00 11400.00 0.00 0.10807 19600.00 11600.00 0.00 0.10840 19800.00 11600.00 0.00 0.10972 20000.00 11600.00 0.00 0.11022 20200.00 11600.00 0.00 0.11024 19600.00 11800.00 0.00 0.11063 19800.00 11800.00 0.00 0.11195 20000.00 11800.00 0.00 0.11245 20200.00 11800.00 0.00 0.11245 19600.00 12000.00 0.00 0.11281 19800.00 12000.00 0.00 0.11412 20000.00 12000.00 0.00 0.11461 19600.00 12200.00 0.00 0.11496 19800.00 12200.00 0.00 0.11626 20000.00 12200.00 0.00 0.11673 19600.00 12400.00 0.00 0.11725 19800.00 12400.00 0.00 0.11853 20000.00 12400.00 0.00 0.11897 19600.00 12600.00 0.00 0.11968 19800.00 12600.00 0.00 0.12095 20000.00 12600.00 0.00 0.12137 19600.00 12800.00 0.00 0.12217 19800.00 12800.00 0.00 0.12343 20000.00 12800.00 0.00 0.12383 19600.00 13000.00 0.00 0.12470 19800.00 13000.00 0.00 0.12596 20000.00 13000.00 0.00 0.12636 19600.00 13200.00 0.00 0.12719 19800.00 13200.00 0.00 0.12847 20000.00 13200.00 0.00 0.12887 19600.00 13400.00 0.00 0.12963 19800.00 13400.00 0.00 0.13091 20000.00 13400.00 0.00 0.13131 19600.00 13600.00 0.00 0.13216 19800.00 13600.00 0.00 0.13346 20000.00 13600.00 0.00 0.13387 19600.00 13800.00 0.00 0.13491 19800.00 13800.00 0.00 0.13624 20000.00 13800.00 0.00 0.13665 19600.00 14000.00 0.00 0.13781 19800.00 14000.00 0.00 0.13917 20000.00 14000.00 0.00 0.13958 19600.00 14200.00 0.00 0.14086 19800.00 14200.00 0.00 0.14224 20000.00 14200.00 0.00 0.14264 19600.00 14400.00 0.00 0.14404 19800.00 14400.00 0.00 0.14541 20000.00 14400.00 0.00 0.14576 19600.00 14600.00 0.00 0.14723 19800.00 14600.00 0.00 0.14856 20000.00 14600.00 0.00 0.14883 19600.00 14800.00 0.00 0.15047 19800.00 14800.00 0.00 0.15173 20000.00 14800.00 0.00 0.15190 19600.00 15000.00 0.00 0.15387 19800.00 15000.00 0.00 0.15505 20000.00 15000.00 0.00 0.15509 19600.00 15200.00 0.00 0.15729 19800.00 15200.00 0.00 0.15837 19600.00 15400.00 0.00 0.16060 19800.00 15400.00 0.00 0.16156 19600.00 15600.00 0.00 0.16382 19800.00 15600.00 0.00 0.16470 19600.00 15800.00 0.00 0.16697 19800.00 15800.00 0.00 0.16776 19400.00 16000.00 0.00 0.16788 19600.00 16000.00 0.00 0.17013 19800.00 16000.00 0.00 0.17085 19400.00 16200.00 0.00 0.17138 19600.00 16200.00 0.00 0.17358 19800.00 16200.00 0.00 0.17421 19400.00 16400.00 0.00 0.17516 19600.00 16400.00 0.00 0.17731 19800.00 16400.00 0.00 0.17783 19400.00 16600.00 0.00 0.17897 19600.00 16600.00 0.00 0.18103 19800.00 16600.00 0.00 0.18143 19400.00 16800.00 0.00 0.18266 19600.00 16800.00 0.00 0.18462 19800.00 16800.00 0.00 0.18486 19400.00 17000.00 0.00 0.18613 19600.00 17000.00 0.00 0.18798 19800.00 17000.00 0.00 0.18806 19400.00 17200.00 0.00 0.18936 19600.00 17200.00 0.00 0.19108 19400.00 17400.00 0.00 0.19264 19600.00 17400.00 0.00 0.19423 19400.00 17600.00 0.00 0.19621 19600.00 17600.00 0.00 0.19764 19400.00 17800.00 0.00 0.19990 19600.00 17800.00 0.00 0.20115 19400.00 18000.00 0.00 0.20355 19600.00 18000.00 0.00 0.20459 19400.00 18200.00 0.00 0.20706 19600.00 18200.00 0.00 0.20785 19400.00 18400.00 0.00 0.21020 19600.00 18400.00 0.00 0.21072 19200.00 18600.00 0.00 0.21074 19400.00 18600.00 0.00 0.21307 19600.00 18600.00 0.00 0.21328 19200.00 18800.00 0.00 0.21392 19400.00 18800.00 0.00 0.21594 19200.00 19000.00 0.00 0.21683 19400.00 19000.00 0.00 0.21843 19200.00 19200.00 0.00 0.21880 19400.00 19200.00 0.00 0.21989 19400.00 19400.00 0.00 0.22006 19200.00 19400.00 30.00 0.22006 19200.00 19400.00 35.00 0.22013 19200.00 19400.00 40.00 0.22019 19200.00 19400.00 45.00 0.22024 19200.00 19400.00 50.00 0.22026 19200.00 19400.00 55.00 0.22027 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19260.03 19445.42 47.98 0.22044 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/473.ctf NX, NY, NZ: 360 360 1 MODE : real