CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/470.mrc Output diagnostic file name Micrographs/470.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/470.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1982.000 7732.000 Mean, RMS : 4366.608 217.5567 TITLE 1: emi2mrc: 470.emi Mon Jan 20 12:40:42 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X -437.568, Y 487.074, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 118 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01343 8400.00 8000.00 0.00 -0.01341 8600.00 8000.00 0.00 -0.01317 8800.00 8000.00 0.00 -0.01291 9000.00 8000.00 0.00 -0.01264 9200.00 8000.00 0.00 -0.01236 9400.00 8000.00 0.00 -0.01201 9600.00 8000.00 0.00 -0.01149 9800.00 8000.00 0.00 -0.01079 10000.00 8000.00 0.00 -0.00997 10200.00 8000.00 0.00 -0.00908 10400.00 8000.00 0.00 -0.00820 10600.00 8000.00 0.00 -0.00735 10800.00 8000.00 0.00 -0.00652 11000.00 8000.00 0.00 -0.00567 11200.00 8000.00 0.00 -0.00480 11400.00 8000.00 0.00 -0.00392 11600.00 8000.00 0.00 -0.00304 11800.00 8000.00 0.00 -0.00218 12000.00 8000.00 0.00 -0.00131 12200.00 8000.00 0.00 -0.00041 12400.00 8000.00 0.00 0.00058 12600.00 8000.00 0.00 0.00167 12800.00 8000.00 0.00 0.00286 13000.00 8000.00 0.00 0.00412 13200.00 8000.00 0.00 0.00544 13400.00 8000.00 0.00 0.00681 13600.00 8000.00 0.00 0.00826 13800.00 8000.00 0.00 0.00982 14000.00 8000.00 0.00 0.01151 14200.00 8000.00 0.00 0.01332 14400.00 8000.00 0.00 0.01522 14600.00 8000.00 0.00 0.01721 14800.00 8000.00 0.00 0.01928 15000.00 8000.00 0.00 0.02145 15200.00 8000.00 0.00 0.02369 15400.00 8000.00 0.00 0.02598 15600.00 8000.00 0.00 0.02826 15800.00 8000.00 0.00 0.03051 16000.00 8000.00 0.00 0.03277 16200.00 8000.00 0.00 0.03511 16400.00 8000.00 0.00 0.03762 16600.00 8000.00 0.00 0.04031 16800.00 8000.00 0.00 0.04311 17000.00 8000.00 0.00 0.04591 17200.00 8000.00 0.00 0.04860 17400.00 8000.00 0.00 0.05115 17600.00 8000.00 0.00 0.05363 17800.00 8000.00 0.00 0.05616 18000.00 8000.00 0.00 0.05880 18200.00 8000.00 0.00 0.06161 18400.00 8000.00 0.00 0.06458 18600.00 8000.00 0.00 0.06769 18800.00 8000.00 0.00 0.07093 19000.00 8000.00 0.00 0.07424 19200.00 8000.00 0.00 0.07747 19400.00 8000.00 0.00 0.08042 19600.00 8000.00 0.00 0.08288 19800.00 8000.00 0.00 0.08470 20000.00 8000.00 0.00 0.08587 20200.00 8000.00 0.00 0.08652 20400.00 8000.00 0.00 0.08681 20600.00 8000.00 0.00 0.08693 20800.00 8000.00 0.00 0.08698 20200.00 8200.00 0.00 0.08760 20400.00 8200.00 0.00 0.08792 20600.00 8200.00 0.00 0.08806 20800.00 8200.00 0.00 0.08812 20200.00 8400.00 0.00 0.08871 20400.00 8400.00 0.00 0.08906 20600.00 8400.00 0.00 0.08921 20800.00 8400.00 0.00 0.08928 20200.00 8600.00 0.00 0.08999 20400.00 8600.00 0.00 0.09035 20600.00 8600.00 0.00 0.09050 20800.00 8600.00 0.00 0.09056 20000.00 8800.00 0.00 0.09064 20200.00 8800.00 0.00 0.09140 20400.00 8800.00 0.00 0.09175 20600.00 8800.00 0.00 0.09187 20800.00 8800.00 0.00 0.09189 20000.00 9000.00 0.00 0.09207 20200.00 9000.00 0.00 0.09281 20400.00 9000.00 0.00 0.09313 20600.00 9000.00 0.00 0.09322 20000.00 9200.00 0.00 0.09348 20200.00 9200.00 0.00 0.09421 20400.00 9200.00 0.00 0.09449 20600.00 9200.00 0.00 0.09452 20000.00 9400.00 0.00 0.09486 20200.00 9400.00 0.00 0.09556 20400.00 9400.00 0.00 0.09580 20000.00 9600.00 0.00 0.09623 20200.00 9600.00 0.00 0.09688 20400.00 9600.00 0.00 0.09707 20000.00 9800.00 0.00 0.09771 20200.00 9800.00 0.00 0.09832 20400.00 9800.00 0.00 0.09844 20000.00 10000.00 0.00 0.09936 20200.00 10000.00 0.00 0.09991 20400.00 10000.00 0.00 0.09997 20000.00 10200.00 0.00 0.10108 20200.00 10200.00 0.00 0.10156 19800.00 10400.00 0.00 0.10169 20000.00 10400.00 0.00 0.10281 20200.00 10400.00 0.00 0.10323 19800.00 10600.00 0.00 0.10347 20000.00 10600.00 0.00 0.10453 20200.00 10600.00 0.00 0.10489 19800.00 10800.00 0.00 0.10522 20000.00 10800.00 0.00 0.10623 20200.00 10800.00 0.00 0.10654 19800.00 11000.00 0.00 0.10704 20000.00 11000.00 0.00 0.10801 20200.00 11000.00 0.00 0.10828 19800.00 11200.00 0.00 0.10904 20000.00 11200.00 0.00 0.10997 20200.00 11200.00 0.00 0.11019 19800.00 11400.00 0.00 0.11110 20000.00 11400.00 0.00 0.11200 20200.00 11400.00 0.00 0.11219 19800.00 11600.00 0.00 0.11315 20000.00 11600.00 0.00 0.11401 20200.00 11600.00 0.00 0.11416 19800.00 11800.00 0.00 0.11512 20000.00 11800.00 0.00 0.11595 20200.00 11800.00 0.00 0.11604 19800.00 12000.00 0.00 0.11696 20000.00 12000.00 0.00 0.11774 20200.00 12000.00 0.00 0.11779 19800.00 12200.00 0.00 0.11876 20000.00 12200.00 0.00 0.11948 19800.00 12400.00 0.00 0.12068 20000.00 12400.00 0.00 0.12135 19800.00 12600.00 0.00 0.12273 20000.00 12600.00 0.00 0.12334 19800.00 12800.00 0.00 0.12482 20000.00 12800.00 0.00 0.12538 19600.00 13000.00 0.00 0.12540 19800.00 13000.00 0.00 0.12695 20000.00 13000.00 0.00 0.12748 19600.00 13200.00 0.00 0.12757 19800.00 13200.00 0.00 0.12910 20000.00 13200.00 0.00 0.12960 19600.00 13400.00 0.00 0.12977 19800.00 13400.00 0.00 0.13129 20000.00 13400.00 0.00 0.13177 19600.00 13600.00 0.00 0.13218 19800.00 13600.00 0.00 0.13370 20000.00 13600.00 0.00 0.13417 19600.00 13800.00 0.00 0.13487 19800.00 13800.00 0.00 0.13639 20000.00 13800.00 0.00 0.13687 19600.00 14000.00 0.00 0.13768 19800.00 14000.00 0.00 0.13923 20000.00 14000.00 0.00 0.13973 19600.00 14200.00 0.00 0.14055 19800.00 14200.00 0.00 0.14212 20000.00 14200.00 0.00 0.14264 19600.00 14400.00 0.00 0.14344 19800.00 14400.00 0.00 0.14504 20000.00 14400.00 0.00 0.14555 19600.00 14600.00 0.00 0.14634 19800.00 14600.00 0.00 0.14794 20000.00 14600.00 0.00 0.14843 19600.00 14800.00 0.00 0.14940 19800.00 14800.00 0.00 0.15099 20000.00 14800.00 0.00 0.15144 19600.00 15000.00 0.00 0.15280 19800.00 15000.00 0.00 0.15436 20000.00 15000.00 0.00 0.15475 19600.00 15200.00 0.00 0.15640 19800.00 15200.00 0.00 0.15791 20000.00 15200.00 0.00 0.15821 19600.00 15400.00 0.00 0.15997 19800.00 15400.00 0.00 0.16141 20000.00 15400.00 0.00 0.16161 19600.00 15600.00 0.00 0.16343 19800.00 15600.00 0.00 0.16480 20000.00 15600.00 0.00 0.16491 19600.00 15800.00 0.00 0.16672 19800.00 15800.00 0.00 0.16802 20000.00 15800.00 0.00 0.16804 19600.00 16000.00 0.00 0.16995 19800.00 16000.00 0.00 0.17119 19600.00 16200.00 0.00 0.17345 19800.00 16200.00 0.00 0.17462 19400.00 16400.00 0.00 0.17470 19600.00 16400.00 0.00 0.17730 19800.00 16400.00 0.00 0.17839 19400.00 16600.00 0.00 0.17869 19600.00 16600.00 0.00 0.18124 19800.00 16600.00 0.00 0.18221 19400.00 16800.00 0.00 0.18258 19600.00 16800.00 0.00 0.18505 19800.00 16800.00 0.00 0.18589 19400.00 17000.00 0.00 0.18618 19600.00 17000.00 0.00 0.18856 19800.00 17000.00 0.00 0.18925 19400.00 17200.00 0.00 0.18942 19600.00 17200.00 0.00 0.19169 19800.00 17200.00 0.00 0.19222 19400.00 17400.00 0.00 0.19261 19600.00 17400.00 0.00 0.19477 19800.00 17400.00 0.00 0.19512 19400.00 17600.00 0.00 0.19605 19600.00 17600.00 0.00 0.19807 19800.00 17600.00 0.00 0.19821 19400.00 17800.00 0.00 0.19959 19600.00 17800.00 0.00 0.20144 19400.00 18000.00 0.00 0.20305 19600.00 18000.00 0.00 0.20471 19400.00 18200.00 0.00 0.20626 19600.00 18200.00 0.00 0.20768 19400.00 18400.00 0.00 0.20898 19600.00 18400.00 0.00 0.21016 19400.00 18600.00 0.00 0.21141 19600.00 18600.00 0.00 0.21232 19400.00 18800.00 0.00 0.21396 19600.00 18800.00 0.00 0.21454 19400.00 19000.00 0.00 0.21645 19600.00 19000.00 0.00 0.21661 19400.00 19200.00 0.00 0.21830 19400.00 19400.00 0.00 0.21917 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19503.58 19329.59 -43.60 0.21945 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/470.ctf NX, NY, NZ: 360 360 1 MODE : real