CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/422.mrc Output diagnostic file name Micrographs/422.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/422.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2224.000 7872.000 Mean, RMS : 4452.571 215.9921 TITLE 1: emi2mrc: 422.emi Mon Jan 20 11:55:19 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X -452.593, Y 485.349, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.08467 8200.00 8000.00 0.00 0.09029 8400.00 8000.00 0.00 0.09589 8600.00 8000.00 0.00 0.10214 8800.00 8000.00 0.00 0.10960 9000.00 8000.00 0.00 0.11796 9200.00 8000.00 0.00 0.12663 9400.00 8000.00 0.00 0.13525 9600.00 8000.00 0.00 0.14360 9800.00 8000.00 0.00 0.15204 10000.00 8000.00 0.00 0.16130 10200.00 8000.00 0.00 0.17142 10400.00 8000.00 0.00 0.18147 10600.00 8000.00 0.00 0.19017 10800.00 8000.00 0.00 0.19619 11000.00 8000.00 0.00 0.19893 11200.00 8000.00 0.00 0.19905 10800.00 8200.00 0.00 0.20281 11000.00 8200.00 0.00 0.20542 10800.00 8400.00 0.00 0.20992 11000.00 8400.00 0.00 0.21235 10800.00 8600.00 0.00 0.21820 11000.00 8600.00 0.00 0.22039 10600.00 8800.00 0.00 0.22180 10800.00 8800.00 0.00 0.22755 11000.00 8800.00 0.00 0.22940 10600.00 9000.00 0.00 0.23166 10800.00 9000.00 0.00 0.23721 11000.00 9000.00 0.00 0.23864 10600.00 9200.00 0.00 0.24145 10800.00 9200.00 0.00 0.24674 11000.00 9200.00 0.00 0.24769 10600.00 9400.00 0.00 0.25092 10800.00 9400.00 0.00 0.25590 11000.00 9400.00 0.00 0.25632 10600.00 9600.00 0.00 0.26027 10800.00 9600.00 0.00 0.26486 10600.00 9800.00 0.00 0.27058 10800.00 9800.00 0.00 0.27458 10600.00 10000.00 0.00 0.28205 10800.00 10000.00 0.00 0.28511 10400.00 10200.00 0.00 0.28578 10600.00 10200.00 0.00 0.29296 10800.00 10200.00 0.00 0.29468 10400.00 10400.00 0.00 0.29534 10600.00 10400.00 0.00 0.30121 10800.00 10400.00 0.00 0.30124 10600.00 10600.00 0.00 0.30513 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10650.42 10609.52 -44.36 0.30534 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/422.ctf NX, NY, NZ: 360 360 1 MODE : real