CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/398.mrc Output diagnostic file name Micrographs/398.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/398.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2318.000 7824.000 Mean, RMS : 4413.259 222.3515 TITLE 1: emi2mrc: 398.emi Mon Jan 20 11:24:15 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X -457.180, Y 492.408, Z -26.464, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00966 8200.00 8000.00 0.00 -0.00960 8400.00 8000.00 0.00 -0.00939 8600.00 8000.00 0.00 -0.00898 8800.00 8000.00 0.00 -0.00840 9000.00 8000.00 0.00 -0.00769 9200.00 8000.00 0.00 -0.00693 9400.00 8000.00 0.00 -0.00619 9600.00 8000.00 0.00 -0.00548 9800.00 8000.00 0.00 -0.00483 10000.00 8000.00 0.00 -0.00424 10200.00 8000.00 0.00 -0.00371 10400.00 8000.00 0.00 -0.00323 10600.00 8000.00 0.00 -0.00277 10800.00 8000.00 0.00 -0.00228 11000.00 8000.00 0.00 -0.00167 11200.00 8000.00 0.00 -0.00092 11400.00 8000.00 0.00 -0.00002 11600.00 8000.00 0.00 0.00098 11800.00 8000.00 0.00 0.00205 12000.00 8000.00 0.00 0.00318 12200.00 8000.00 0.00 0.00440 12400.00 8000.00 0.00 0.00575 12600.00 8000.00 0.00 0.00727 12800.00 8000.00 0.00 0.00892 13000.00 8000.00 0.00 0.01065 13200.00 8000.00 0.00 0.01242 13400.00 8000.00 0.00 0.01419 13600.00 8000.00 0.00 0.01599 13800.00 8000.00 0.00 0.01786 14000.00 8000.00 0.00 0.01984 14200.00 8000.00 0.00 0.02197 14400.00 8000.00 0.00 0.02430 14600.00 8000.00 0.00 0.02689 14800.00 8000.00 0.00 0.02976 15000.00 8000.00 0.00 0.03288 15200.00 8000.00 0.00 0.03614 15400.00 8000.00 0.00 0.03938 15600.00 8000.00 0.00 0.04251 15800.00 8000.00 0.00 0.04549 16000.00 8000.00 0.00 0.04843 16200.00 8000.00 0.00 0.05148 16400.00 8000.00 0.00 0.05474 16600.00 8000.00 0.00 0.05815 16800.00 8000.00 0.00 0.06162 17000.00 8000.00 0.00 0.06507 17200.00 8000.00 0.00 0.06855 17400.00 8000.00 0.00 0.07218 17600.00 8000.00 0.00 0.07615 17800.00 8000.00 0.00 0.08037 18000.00 8000.00 0.00 0.08449 18200.00 8000.00 0.00 0.08806 18400.00 8000.00 0.00 0.09062 18600.00 8000.00 0.00 0.09201 18800.00 8000.00 0.00 0.09248 18600.00 8200.00 0.00 0.09297 18800.00 8200.00 0.00 0.09344 18600.00 8400.00 0.00 0.09409 18800.00 8400.00 0.00 0.09458 18600.00 8600.00 0.00 0.09553 18800.00 8600.00 0.00 0.09606 19000.00 8600.00 0.00 0.09608 19600.00 8600.00 0.00 0.09615 19800.00 8600.00 0.00 0.09621 18600.00 8800.00 0.00 0.09726 18800.00 8800.00 0.00 0.09781 19000.00 8800.00 0.00 0.09787 19400.00 8800.00 0.00 0.09789 19600.00 8800.00 0.00 0.09806 19800.00 8800.00 0.00 0.09816 18600.00 9000.00 0.00 0.09908 18800.00 9000.00 0.00 0.09965 19000.00 9000.00 0.00 0.09973 19400.00 9000.00 0.00 0.09981 19600.00 9000.00 0.00 0.10000 19800.00 9000.00 0.00 0.10011 18600.00 9200.00 0.00 0.10088 18800.00 9200.00 0.00 0.10146 19000.00 9200.00 0.00 0.10155 19400.00 9200.00 0.00 0.10166 19600.00 9200.00 0.00 0.10185 19800.00 9200.00 0.00 0.10195 18600.00 9400.00 0.00 0.10261 18800.00 9400.00 0.00 0.10320 19000.00 9400.00 0.00 0.10329 19400.00 9400.00 0.00 0.10338 19600.00 9400.00 0.00 0.10357 19800.00 9400.00 0.00 0.10364 18600.00 9600.00 0.00 0.10431 18800.00 9600.00 0.00 0.10490 19000.00 9600.00 0.00 0.10497 19400.00 9600.00 0.00 0.10502 19600.00 9600.00 0.00 0.10519 19800.00 9600.00 0.00 0.10524 18600.00 9800.00 0.00 0.10614 18800.00 9800.00 0.00 0.10670 19000.00 9800.00 0.00 0.10675 19400.00 9800.00 0.00 0.10676 19600.00 9800.00 0.00 0.10691 19800.00 9800.00 0.00 0.10694 18600.00 10000.00 0.00 0.10813 18800.00 10000.00 0.00 0.10867 19000.00 10000.00 0.00 0.10869 19600.00 10000.00 0.00 0.10880 19800.00 10000.00 0.00 0.10882 18600.00 10200.00 0.00 0.11015 18800.00 10200.00 0.00 0.11067 19600.00 10200.00 0.00 0.11074 18600.00 10400.00 0.00 0.11216 18800.00 10400.00 0.00 0.11266 18600.00 10600.00 0.00 0.11415 18800.00 10600.00 0.00 0.11461 18400.00 10800.00 0.00 0.11466 18600.00 10800.00 0.00 0.11614 18800.00 10800.00 0.00 0.11655 18400.00 11000.00 0.00 0.11686 18600.00 11000.00 0.00 0.11829 18800.00 11000.00 0.00 0.11866 18400.00 11200.00 0.00 0.11935 18600.00 11200.00 0.00 0.12073 18800.00 11200.00 0.00 0.12104 18400.00 11400.00 0.00 0.12196 18600.00 11400.00 0.00 0.12328 18800.00 11400.00 0.00 0.12354 18400.00 11600.00 0.00 0.12451 18600.00 11600.00 0.00 0.12579 18800.00 11600.00 0.00 0.12603 18400.00 11800.00 0.00 0.12699 18600.00 11800.00 0.00 0.12825 18800.00 11800.00 0.00 0.12846 18400.00 12000.00 0.00 0.12942 18600.00 12000.00 0.00 0.13065 18800.00 12000.00 0.00 0.13084 18400.00 12200.00 0.00 0.13194 18600.00 12200.00 0.00 0.13315 18800.00 12200.00 0.00 0.13330 18400.00 12400.00 0.00 0.13480 18600.00 12400.00 0.00 0.13598 18800.00 12400.00 0.00 0.13610 18400.00 12600.00 0.00 0.13794 18600.00 12600.00 0.00 0.13909 18800.00 12600.00 0.00 0.13918 18400.00 12800.00 0.00 0.14111 18600.00 12800.00 0.00 0.14223 18800.00 12800.00 0.00 0.14228 18400.00 13000.00 0.00 0.14419 18600.00 13000.00 0.00 0.14528 18800.00 13000.00 0.00 0.14529 18400.00 13200.00 0.00 0.14712 18600.00 13200.00 0.00 0.14819 18400.00 13400.00 0.00 0.14999 18600.00 13400.00 0.00 0.15104 18400.00 13600.00 0.00 0.15316 18600.00 13600.00 0.00 0.15420 18400.00 13800.00 0.00 0.15681 18600.00 13800.00 0.00 0.15784 18200.00 14000.00 0.00 0.15807 18400.00 14000.00 0.00 0.16073 18600.00 14000.00 0.00 0.16173 18200.00 14200.00 0.00 0.16203 18400.00 14200.00 0.00 0.16470 18600.00 14200.00 0.00 0.16565 18200.00 14400.00 0.00 0.16594 18400.00 14400.00 0.00 0.16860 18600.00 14400.00 0.00 0.16950 18200.00 14600.00 0.00 0.16973 18400.00 14600.00 0.00 0.17236 18600.00 14600.00 0.00 0.17318 18200.00 14800.00 0.00 0.17362 18400.00 14800.00 0.00 0.17621 18600.00 14800.00 0.00 0.17693 18200.00 15000.00 0.00 0.17793 18400.00 15000.00 0.00 0.18046 18600.00 15000.00 0.00 0.18108 18200.00 15200.00 0.00 0.18255 18400.00 15200.00 0.00 0.18502 18600.00 15200.00 0.00 0.18551 18200.00 15400.00 0.00 0.18721 18400.00 15400.00 0.00 0.18960 18600.00 15400.00 0.00 0.18996 18200.00 15600.00 0.00 0.19174 18400.00 15600.00 0.00 0.19405 18600.00 15600.00 0.00 0.19427 18200.00 15800.00 0.00 0.19601 18400.00 15800.00 0.00 0.19824 18600.00 15800.00 0.00 0.19833 18200.00 16000.00 0.00 0.20014 18400.00 16000.00 0.00 0.20229 18200.00 16200.00 0.00 0.20458 18400.00 16200.00 0.00 0.20664 18200.00 16400.00 0.00 0.20943 18400.00 16400.00 0.00 0.21138 18200.00 16600.00 0.00 0.21439 18400.00 16600.00 0.00 0.21618 18200.00 16800.00 0.00 0.21924 18400.00 16800.00 0.00 0.22083 18200.00 17000.00 0.00 0.22380 18400.00 17000.00 0.00 0.22512 18200.00 17200.00 0.00 0.22798 18400.00 17200.00 0.00 0.22898 18200.00 17400.00 0.00 0.23222 18400.00 17400.00 0.00 0.23280 18000.00 17600.00 0.00 0.23358 18200.00 17600.00 0.00 0.23671 18000.00 17800.00 0.00 0.23817 18200.00 17800.00 0.00 0.24071 18000.00 18000.00 0.00 0.24152 18200.00 18000.00 0.00 0.24332 18200.00 18200.00 0.00 0.24393 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18110.44 18216.24 45.57 0.24421 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/398.ctf NX, NY, NZ: 360 360 1 MODE : real