CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/386.mrc Output diagnostic file name Micrographs/386.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/386.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2408.000 7796.000 Mean, RMS : 4509.590 229.0418 TITLE 1: emi2mrc: 386.emi Mon Jan 20 11:13:09 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.07%, Obj 88.07%, Dif 73.67% TITLE 3: X -439.411, Y 505.390, Z -26.464, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01601 8200.00 8000.00 0.00 -0.01564 8400.00 8000.00 0.00 -0.01513 8600.00 8000.00 0.00 -0.01459 8800.00 8000.00 0.00 -0.01409 9000.00 8000.00 0.00 -0.01359 9200.00 8000.00 0.00 -0.01310 9400.00 8000.00 0.00 -0.01260 9600.00 8000.00 0.00 -0.01208 9800.00 8000.00 0.00 -0.01154 10000.00 8000.00 0.00 -0.01097 10200.00 8000.00 0.00 -0.01034 10400.00 8000.00 0.00 -0.00961 10600.00 8000.00 0.00 -0.00878 10800.00 8000.00 0.00 -0.00784 11000.00 8000.00 0.00 -0.00680 11200.00 8000.00 0.00 -0.00564 11400.00 8000.00 0.00 -0.00434 11600.00 8000.00 0.00 -0.00289 11800.00 8000.00 0.00 -0.00134 12000.00 8000.00 0.00 0.00024 12200.00 8000.00 0.00 0.00177 12400.00 8000.00 0.00 0.00321 12600.00 8000.00 0.00 0.00461 12800.00 8000.00 0.00 0.00603 13000.00 8000.00 0.00 0.00755 13200.00 8000.00 0.00 0.00916 13400.00 8000.00 0.00 0.01086 13600.00 8000.00 0.00 0.01263 13800.00 8000.00 0.00 0.01448 14000.00 8000.00 0.00 0.01647 14200.00 8000.00 0.00 0.01860 14400.00 8000.00 0.00 0.02084 14600.00 8000.00 0.00 0.02313 14800.00 8000.00 0.00 0.02541 15000.00 8000.00 0.00 0.02769 15200.00 8000.00 0.00 0.03006 15400.00 8000.00 0.00 0.03258 15600.00 8000.00 0.00 0.03530 15800.00 8000.00 0.00 0.03815 16000.00 8000.00 0.00 0.04105 16200.00 8000.00 0.00 0.04394 16400.00 8000.00 0.00 0.04685 16600.00 8000.00 0.00 0.04981 16800.00 8000.00 0.00 0.05285 17000.00 8000.00 0.00 0.05596 17200.00 8000.00 0.00 0.05906 17400.00 8000.00 0.00 0.06215 17600.00 8000.00 0.00 0.06533 17800.00 8000.00 0.00 0.06876 18000.00 8000.00 0.00 0.07253 18200.00 8000.00 0.00 0.07662 18400.00 8000.00 0.00 0.08075 18600.00 8000.00 0.00 0.08454 18800.00 8000.00 0.00 0.08766 19000.00 8000.00 0.00 0.08994 19200.00 8000.00 0.00 0.09140 19400.00 8000.00 0.00 0.09221 19600.00 8000.00 0.00 0.09262 19800.00 8000.00 0.00 0.09277 20000.00 8000.00 0.00 0.09279 19400.00 8200.00 0.00 0.09327 19600.00 8200.00 0.00 0.09366 19800.00 8200.00 0.00 0.09382 20000.00 8200.00 0.00 0.09384 19400.00 8400.00 0.00 0.09437 19600.00 8400.00 0.00 0.09477 19800.00 8400.00 0.00 0.09494 20000.00 8400.00 0.00 0.09499 19400.00 8600.00 0.00 0.09568 19600.00 8600.00 0.00 0.09610 19800.00 8600.00 0.00 0.09630 20000.00 8600.00 0.00 0.09639 20200.00 8600.00 0.00 0.09639 19400.00 8800.00 0.00 0.09719 19600.00 8800.00 0.00 0.09764 19800.00 8800.00 0.00 0.09787 20000.00 8800.00 0.00 0.09798 20200.00 8800.00 0.00 0.09800 19400.00 9000.00 0.00 0.09875 19600.00 9000.00 0.00 0.09922 19800.00 9000.00 0.00 0.09946 20000.00 9000.00 0.00 0.09957 20200.00 9000.00 0.00 0.09958 19400.00 9200.00 0.00 0.10027 19600.00 9200.00 0.00 0.10075 19800.00 9200.00 0.00 0.10098 20000.00 9200.00 0.00 0.10108 19400.00 9400.00 0.00 0.10170 19600.00 9400.00 0.00 0.10216 19800.00 9400.00 0.00 0.10237 20000.00 9400.00 0.00 0.10243 19400.00 9600.00 0.00 0.10303 19600.00 9600.00 0.00 0.10347 19800.00 9600.00 0.00 0.10364 20000.00 9600.00 0.00 0.10367 19400.00 9800.00 0.00 0.10445 19600.00 9800.00 0.00 0.10486 19800.00 9800.00 0.00 0.10500 19200.00 10000.00 0.00 0.10525 19400.00 10000.00 0.00 0.10606 19600.00 10000.00 0.00 0.10645 19800.00 10000.00 0.00 0.10656 19200.00 10200.00 0.00 0.10701 19400.00 10200.00 0.00 0.10780 19600.00 10200.00 0.00 0.10816 19800.00 10200.00 0.00 0.10823 19200.00 10400.00 0.00 0.10882 19400.00 10400.00 0.00 0.10959 19600.00 10400.00 0.00 0.10992 19800.00 10400.00 0.00 0.10997 19200.00 10600.00 0.00 0.11063 19400.00 10600.00 0.00 0.11138 19600.00 10600.00 0.00 0.11169 19800.00 10600.00 0.00 0.11172 19200.00 10800.00 0.00 0.11239 19400.00 10800.00 0.00 0.11313 19600.00 10800.00 0.00 0.11343 19800.00 10800.00 0.00 0.11344 19200.00 11000.00 0.00 0.11424 19400.00 11000.00 0.00 0.11497 19600.00 11000.00 0.00 0.11526 19200.00 11200.00 0.00 0.11631 19400.00 11200.00 0.00 0.11703 19600.00 11200.00 0.00 0.11730 19200.00 11400.00 0.00 0.11856 19400.00 11400.00 0.00 0.11927 19600.00 11400.00 0.00 0.11950 19200.00 11600.00 0.00 0.12090 19400.00 11600.00 0.00 0.12159 19600.00 11600.00 0.00 0.12178 19000.00 11800.00 0.00 0.12191 19200.00 11800.00 0.00 0.12331 19400.00 11800.00 0.00 0.12396 19600.00 11800.00 0.00 0.12411 19000.00 12000.00 0.00 0.12431 19200.00 12000.00 0.00 0.12569 19400.00 12000.00 0.00 0.12630 19600.00 12000.00 0.00 0.12639 19000.00 12200.00 0.00 0.12672 19200.00 12200.00 0.00 0.12806 19400.00 12200.00 0.00 0.12862 19600.00 12200.00 0.00 0.12865 19000.00 12400.00 0.00 0.12928 19200.00 12400.00 0.00 0.13058 19400.00 12400.00 0.00 0.13109 19000.00 12600.00 0.00 0.13196 19200.00 12600.00 0.00 0.13322 19400.00 12600.00 0.00 0.13365 19000.00 12800.00 0.00 0.13462 19200.00 12800.00 0.00 0.13582 19400.00 12800.00 0.00 0.13619 19000.00 13000.00 0.00 0.13725 19200.00 13000.00 0.00 0.13840 19400.00 13000.00 0.00 0.13870 19000.00 13200.00 0.00 0.13983 19200.00 13200.00 0.00 0.14094 19400.00 13200.00 0.00 0.14120 19000.00 13400.00 0.00 0.14243 19200.00 13400.00 0.00 0.14350 19400.00 13400.00 0.00 0.14373 19000.00 13600.00 0.00 0.14529 19200.00 13600.00 0.00 0.14633 19400.00 13600.00 0.00 0.14652 19000.00 13800.00 0.00 0.14851 19200.00 13800.00 0.00 0.14951 19400.00 13800.00 0.00 0.14965 18800.00 14000.00 0.00 0.14980 19000.00 14000.00 0.00 0.15191 19200.00 14000.00 0.00 0.15286 19400.00 14000.00 0.00 0.15294 18800.00 14200.00 0.00 0.15328 19000.00 14200.00 0.00 0.15536 19200.00 14200.00 0.00 0.15625 19400.00 14200.00 0.00 0.15626 18800.00 14400.00 0.00 0.15673 19000.00 14400.00 0.00 0.15876 19200.00 14400.00 0.00 0.15960 18800.00 14600.00 0.00 0.16004 19000.00 14600.00 0.00 0.16202 19200.00 14600.00 0.00 0.16279 18800.00 14800.00 0.00 0.16336 19000.00 14800.00 0.00 0.16529 19200.00 14800.00 0.00 0.16600 18800.00 15000.00 0.00 0.16697 19000.00 15000.00 0.00 0.16885 19200.00 15000.00 0.00 0.16950 18800.00 15200.00 0.00 0.17079 19000.00 15200.00 0.00 0.17262 19200.00 15200.00 0.00 0.17321 18800.00 15400.00 0.00 0.17467 19000.00 15400.00 0.00 0.17645 19200.00 15400.00 0.00 0.17697 18800.00 15600.00 0.00 0.17854 19000.00 15600.00 0.00 0.18028 19200.00 15600.00 0.00 0.18074 18800.00 15800.00 0.00 0.18230 19000.00 15800.00 0.00 0.18401 19200.00 15800.00 0.00 0.18439 18800.00 16000.00 0.00 0.18602 19000.00 16000.00 0.00 0.18766 19200.00 16000.00 0.00 0.18794 18800.00 16200.00 0.00 0.18996 19000.00 16200.00 0.00 0.19152 19200.00 16200.00 0.00 0.19166 18800.00 16400.00 0.00 0.19417 19000.00 16400.00 0.00 0.19560 18800.00 16600.00 0.00 0.19837 19000.00 16600.00 0.00 0.19964 18600.00 16800.00 0.00 0.19968 18800.00 16800.00 0.00 0.20241 19000.00 16800.00 0.00 0.20348 18600.00 17000.00 0.00 0.20357 18800.00 17000.00 0.00 0.20616 19000.00 17000.00 0.00 0.20704 18600.00 17200.00 0.00 0.20718 18800.00 17200.00 0.00 0.20962 19000.00 17200.00 0.00 0.21031 18600.00 17400.00 0.00 0.21097 18800.00 17400.00 0.00 0.21324 19000.00 17400.00 0.00 0.21368 18600.00 17600.00 0.00 0.21532 18800.00 17600.00 0.00 0.21732 19000.00 17600.00 0.00 0.21743 18600.00 17800.00 0.00 0.21996 18800.00 17800.00 0.00 0.22157 18600.00 18000.00 0.00 0.22447 18800.00 18000.00 0.00 0.22557 18400.00 18200.00 0.00 0.22560 18600.00 18200.00 0.00 0.22832 18800.00 18200.00 0.00 0.22879 18600.00 18400.00 0.00 0.23066 18600.00 18600.00 0.00 0.23123 18600.00 18800.00 35.00 0.23128 18400.00 18800.00 40.00 0.23129 18600.00 18800.00 40.00 0.23130 18400.00 18800.00 45.00 0.23137 18400.00 18800.00 50.00 0.23141 18400.00 18800.00 55.00 0.23142 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18495.78 18749.27 50.34 0.23166 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/386.ctf NX, NY, NZ: 360 360 1 MODE : real