CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/358.mrc Output diagnostic file name Micrographs/358.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/358.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2420.000 8738.000 Mean, RMS : 4376.775 228.2968 TITLE 1: emi2mrc: 358.emi Mon Jan 20 01:19:34 2014 TITLE 2: kV 300, Mag 78000x, Def 24450, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X -510.114, Y 534.982, Z -22.878, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03429 15400.00 8000.00 0.00 -0.03402 15600.00 8000.00 0.00 -0.03339 15800.00 8000.00 0.00 -0.03272 16000.00 8000.00 0.00 -0.03204 16200.00 8000.00 0.00 -0.03137 16400.00 8000.00 0.00 -0.03073 16600.00 8000.00 0.00 -0.03004 16800.00 8000.00 0.00 -0.02928 17000.00 8000.00 0.00 -0.02846 17200.00 8000.00 0.00 -0.02764 17400.00 8000.00 0.00 -0.02686 17600.00 8000.00 0.00 -0.02616 17800.00 8000.00 0.00 -0.02544 18000.00 8000.00 0.00 -0.02460 18200.00 8000.00 0.00 -0.02355 18400.00 8000.00 0.00 -0.02231 18600.00 8000.00 0.00 -0.02096 18800.00 8000.00 0.00 -0.01963 19000.00 8000.00 0.00 -0.01838 19200.00 8000.00 0.00 -0.01715 19400.00 8000.00 0.00 -0.01587 19600.00 8000.00 0.00 -0.01447 19800.00 8000.00 0.00 -0.01298 20000.00 8000.00 0.00 -0.01148 20200.00 8000.00 0.00 -0.01009 20400.00 8000.00 0.00 -0.00884 20600.00 8000.00 0.00 -0.00771 20800.00 8000.00 0.00 -0.00661 21000.00 8000.00 0.00 -0.00544 21200.00 8000.00 0.00 -0.00415 21400.00 8000.00 0.00 -0.00269 21600.00 8000.00 0.00 -0.00107 21800.00 8000.00 0.00 0.00070 22000.00 8000.00 0.00 0.00259 22200.00 8000.00 0.00 0.00454 22400.00 8000.00 0.00 0.00645 22600.00 8000.00 0.00 0.00828 22800.00 8000.00 0.00 0.01002 23000.00 8000.00 0.00 0.01174 23200.00 8000.00 0.00 0.01352 23400.00 8000.00 0.00 0.01546 23600.00 8000.00 0.00 0.01759 23800.00 8000.00 0.00 0.01988 24000.00 8000.00 0.00 0.02232 24200.00 8000.00 0.00 0.02490 24400.00 8000.00 0.00 0.02766 24600.00 8000.00 0.00 0.03062 24800.00 8000.00 0.00 0.03370 25000.00 8000.00 0.00 0.03672 25200.00 8000.00 0.00 0.03945 25400.00 8000.00 0.00 0.04161 25600.00 8000.00 0.00 0.04308 25800.00 8000.00 0.00 0.04390 26000.00 8000.00 0.00 0.04421 25800.00 8200.00 0.00 0.04451 26000.00 8200.00 0.00 0.04479 25800.00 8400.00 0.00 0.04506 26000.00 8400.00 0.00 0.04533 25800.00 8600.00 0.00 0.04556 26000.00 8600.00 0.00 0.04581 25800.00 8800.00 0.00 0.04599 26000.00 8800.00 0.00 0.04622 25800.00 9000.00 0.00 0.04636 26000.00 9000.00 0.00 0.04659 25800.00 9200.00 0.00 0.04674 26000.00 9200.00 0.00 0.04698 25800.00 9400.00 0.00 0.04719 26000.00 9400.00 0.00 0.04744 25800.00 9600.00 0.00 0.04771 26000.00 9600.00 0.00 0.04797 25800.00 9800.00 0.00 0.04832 26000.00 9800.00 0.00 0.04858 25800.00 10000.00 0.00 0.04899 26000.00 10000.00 0.00 0.04925 25800.00 10200.00 0.00 0.04964 26000.00 10200.00 0.00 0.04990 25800.00 10400.00 0.00 0.05023 26000.00 10400.00 0.00 0.05050 25800.00 10600.00 0.00 0.05078 26000.00 10600.00 0.00 0.05105 25800.00 10800.00 0.00 0.05132 26000.00 10800.00 0.00 0.05159 25800.00 11000.00 0.00 0.05195 26000.00 11000.00 0.00 0.05223 25800.00 11200.00 0.00 0.05275 26000.00 11200.00 0.00 0.05303 25800.00 11400.00 0.00 0.05372 26000.00 11400.00 0.00 0.05400 25800.00 11600.00 0.00 0.05480 26000.00 11600.00 0.00 0.05508 25600.00 11800.00 0.00 0.05513 25800.00 11800.00 0.00 0.05594 26000.00 11800.00 0.00 0.05620 25600.00 12000.00 0.00 0.05625 25800.00 12000.00 0.00 0.05704 26000.00 12000.00 0.00 0.05728 25600.00 12200.00 0.00 0.05729 25800.00 12200.00 0.00 0.05806 26000.00 12200.00 0.00 0.05827 25600.00 12400.00 0.00 0.05827 25800.00 12400.00 0.00 0.05902 26000.00 12400.00 0.00 0.05918 25600.00 12600.00 0.00 0.05919 25800.00 12600.00 0.00 0.05990 26000.00 12600.00 0.00 0.06001 25600.00 12800.00 0.00 0.06003 25800.00 12800.00 0.00 0.06070 26000.00 12800.00 0.00 0.06076 25600.00 13000.00 0.00 0.06087 25800.00 13000.00 0.00 0.06149 26000.00 13000.00 0.00 0.06151 25600.00 13200.00 0.00 0.06173 25800.00 13200.00 0.00 0.06231 25600.00 13400.00 0.00 0.06263 25800.00 13400.00 0.00 0.06320 25600.00 13600.00 0.00 0.06364 25800.00 13600.00 0.00 0.06419 25600.00 13800.00 0.00 0.06473 25800.00 13800.00 0.00 0.06527 25600.00 14000.00 0.00 0.06583 25800.00 14000.00 0.00 0.06637 25600.00 14200.00 0.00 0.06691 25800.00 14200.00 0.00 0.06745 25600.00 14400.00 0.00 0.06800 25800.00 14400.00 0.00 0.06855 25600.00 14600.00 0.00 0.06914 25800.00 14600.00 0.00 0.06969 25600.00 14800.00 0.00 0.07039 25800.00 14800.00 0.00 0.07094 25600.00 15000.00 0.00 0.07183 25800.00 15000.00 0.00 0.07237 25600.00 15200.00 0.00 0.07338 25800.00 15200.00 0.00 0.07392 25600.00 15400.00 0.00 0.07497 25800.00 15400.00 0.00 0.07550 25600.00 15600.00 0.00 0.07655 25800.00 15600.00 0.00 0.07707 25600.00 15800.00 0.00 0.07811 25800.00 15800.00 0.00 0.07860 25600.00 16000.00 0.00 0.07968 25800.00 16000.00 0.00 0.08012 25600.00 16200.00 0.00 0.08137 25800.00 16200.00 0.00 0.08176 25400.00 16400.00 0.00 0.08184 25600.00 16400.00 0.00 0.08318 25800.00 16400.00 0.00 0.08351 25400.00 16600.00 0.00 0.08375 25600.00 16600.00 0.00 0.08504 25800.00 16600.00 0.00 0.08532 25400.00 16800.00 0.00 0.08565 25600.00 16800.00 0.00 0.08689 25800.00 16800.00 0.00 0.08710 25400.00 17000.00 0.00 0.08747 25600.00 17000.00 0.00 0.08866 25800.00 17000.00 0.00 0.08881 25400.00 17200.00 0.00 0.08920 25600.00 17200.00 0.00 0.09034 25800.00 17200.00 0.00 0.09044 25400.00 17400.00 0.00 0.09094 25600.00 17400.00 0.00 0.09206 25800.00 17400.00 0.00 0.09213 25400.00 17600.00 0.00 0.09280 25600.00 17600.00 0.00 0.09389 25800.00 17600.00 0.00 0.09394 25400.00 17800.00 0.00 0.09474 25600.00 17800.00 0.00 0.09582 25800.00 17800.00 0.00 0.09584 25400.00 18000.00 0.00 0.09675 25600.00 18000.00 0.00 0.09780 25400.00 18200.00 0.00 0.09878 25600.00 18200.00 0.00 0.09981 25400.00 18400.00 0.00 0.10078 25600.00 18400.00 0.00 0.10177 25400.00 18600.00 0.00 0.10277 25600.00 18600.00 0.00 0.10373 25400.00 18800.00 0.00 0.10486 25600.00 18800.00 0.00 0.10579 25400.00 19000.00 0.00 0.10701 25600.00 19000.00 0.00 0.10790 25400.00 19200.00 0.00 0.10914 25600.00 19200.00 0.00 0.11000 25400.00 19400.00 0.00 0.11125 25600.00 19400.00 0.00 0.11208 25400.00 19600.00 0.00 0.11330 25600.00 19600.00 0.00 0.11412 25400.00 19800.00 0.00 0.11533 25600.00 19800.00 0.00 0.11616 25400.00 20000.00 0.00 0.11751 25600.00 20000.00 0.00 0.11834 25400.00 20200.00 0.00 0.11987 25600.00 20200.00 0.00 0.12069 25400.00 20400.00 0.00 0.12232 25600.00 20400.00 0.00 0.12314 25400.00 20600.00 0.00 0.12487 25600.00 20600.00 0.00 0.12567 25400.00 20800.00 0.00 0.12749 25600.00 20800.00 0.00 0.12826 25400.00 21000.00 0.00 0.13011 25600.00 21000.00 0.00 0.13084 25400.00 21200.00 0.00 0.13285 25600.00 21200.00 0.00 0.13351 25200.00 21400.00 0.00 0.13365 25400.00 21400.00 0.00 0.13579 25600.00 21400.00 0.00 0.13637 25200.00 21600.00 0.00 0.13671 25400.00 21600.00 0.00 0.13878 25600.00 21600.00 0.00 0.13926 25200.00 21800.00 0.00 0.13970 25400.00 21800.00 0.00 0.14170 25600.00 21800.00 0.00 0.14207 25200.00 22000.00 0.00 0.14260 25400.00 22000.00 0.00 0.14452 25600.00 22000.00 0.00 0.14479 25200.00 22200.00 0.00 0.14534 25400.00 22200.00 0.00 0.14719 25600.00 22200.00 0.00 0.14737 25200.00 22400.00 0.00 0.14806 25400.00 22400.00 0.00 0.14984 25600.00 22400.00 0.00 0.14991 25200.00 22600.00 0.00 0.15100 25400.00 22600.00 0.00 0.15271 25200.00 22800.00 0.00 0.15421 25400.00 22800.00 0.00 0.15584 25200.00 23000.00 0.00 0.15755 25400.00 23000.00 0.00 0.15908 25200.00 23200.00 0.00 0.16093 25400.00 23200.00 0.00 0.16236 25200.00 23400.00 0.00 0.16421 25400.00 23400.00 0.00 0.16552 25200.00 23600.00 0.00 0.16733 25400.00 23600.00 0.00 0.16851 25200.00 23800.00 0.00 0.17060 25400.00 23800.00 0.00 0.17160 25200.00 24000.00 0.00 0.17419 25400.00 24000.00 0.00 0.17493 25000.00 24200.00 0.00 0.17542 25200.00 24200.00 0.00 0.17797 25400.00 24200.00 0.00 0.17840 25000.00 24400.00 0.00 0.17961 25200.00 24400.00 0.00 0.18182 25400.00 24400.00 0.00 0.18186 25000.00 24600.00 0.00 0.18360 25200.00 24600.00 0.00 0.18537 25000.00 24800.00 0.00 0.18665 25200.00 24800.00 0.00 0.18792 25000.00 25000.00 0.00 0.18843 25200.00 25000.00 0.00 0.18921 25200.00 25200.00 0.00 0.18926 25000.00 25200.00 15.00 0.18938 25000.00 25200.00 20.00 0.18950 25000.00 25200.00 25.00 0.18960 25000.00 25200.00 30.00 0.18970 25000.00 25200.00 35.00 0.18977 25000.00 25200.00 40.00 0.18983 25000.00 25200.00 45.00 0.18986 25000.00 25200.00 50.00 0.18986 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25000.00 25200.00 50.00 0.18986 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/358.ctf NX, NY, NZ: 360 360 1 MODE : real