CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/214.mrc Output diagnostic file name Micrographs/214.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/214.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2276.000 7806.000 Mean, RMS : 4432.976 219.1288 TITLE 1: emi2mrc: 214.emi Sun Jan 19 21:26:19 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 113.069, Y 687.102, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03806 8200.00 8000.00 0.00 0.04017 8400.00 8000.00 0.00 0.04216 8600.00 8000.00 0.00 0.04448 8800.00 8000.00 0.00 0.04758 9000.00 8000.00 0.00 0.05150 9200.00 8000.00 0.00 0.05600 9400.00 8000.00 0.00 0.06079 9600.00 8000.00 0.00 0.06560 9800.00 8000.00 0.00 0.07051 10000.00 8000.00 0.00 0.07600 10200.00 8000.00 0.00 0.08237 10400.00 8000.00 0.00 0.08952 10600.00 8000.00 0.00 0.09711 10800.00 8000.00 0.00 0.10465 11000.00 8000.00 0.00 0.11192 11200.00 8000.00 0.00 0.11928 11400.00 8000.00 0.00 0.12728 11600.00 8000.00 0.00 0.13606 11800.00 8000.00 0.00 0.14524 12000.00 8000.00 0.00 0.15387 12200.00 8000.00 0.00 0.16077 12400.00 8000.00 0.00 0.16527 12600.00 8000.00 0.00 0.16748 12800.00 8000.00 0.00 0.16794 12400.00 8200.00 0.00 0.16964 12600.00 8200.00 0.00 0.17180 12800.00 8200.00 0.00 0.17218 12400.00 8400.00 0.00 0.17421 12600.00 8400.00 0.00 0.17631 12800.00 8400.00 0.00 0.17659 12400.00 8600.00 0.00 0.17959 12600.00 8600.00 0.00 0.18160 12800.00 8600.00 0.00 0.18176 12400.00 8800.00 0.00 0.18583 12600.00 8800.00 0.00 0.18771 12200.00 9000.00 0.00 0.18818 12400.00 9000.00 0.00 0.19248 12600.00 9000.00 0.00 0.19421 12200.00 9200.00 0.00 0.19501 12400.00 9200.00 0.00 0.19918 12600.00 9200.00 0.00 0.20072 12200.00 9400.00 0.00 0.20159 12400.00 9400.00 0.00 0.20562 12600.00 9400.00 0.00 0.20695 12200.00 9600.00 0.00 0.20794 12400.00 9600.00 0.00 0.21179 12600.00 9600.00 0.00 0.21290 12200.00 9800.00 0.00 0.21472 12400.00 9800.00 0.00 0.21839 12600.00 9800.00 0.00 0.21924 12200.00 10000.00 0.00 0.22242 12400.00 10000.00 0.00 0.22586 12600.00 10000.00 0.00 0.22640 12200.00 10200.00 0.00 0.23071 12400.00 10200.00 0.00 0.23388 12600.00 10200.00 0.00 0.23409 12200.00 10400.00 0.00 0.23917 12400.00 10400.00 0.00 0.24204 12200.00 10600.00 0.00 0.24741 12400.00 10600.00 0.00 0.24996 12200.00 10800.00 0.00 0.25520 12400.00 10800.00 0.00 0.25741 12200.00 11000.00 0.00 0.26332 12400.00 11000.00 0.00 0.26509 12000.00 11200.00 0.00 0.26713 12200.00 11200.00 0.00 0.27279 12400.00 11200.00 0.00 0.27387 12000.00 11400.00 0.00 0.27839 12200.00 11400.00 0.00 0.28330 12400.00 11400.00 0.00 0.28336 12000.00 11600.00 0.00 0.28983 12200.00 11600.00 0.00 0.29358 12000.00 11800.00 0.00 0.29984 12200.00 11800.00 0.00 0.30197 12000.00 12000.00 0.00 0.30599 12200.00 12000.00 0.00 0.30619 12000.00 12200.00 0.00 0.30660 12000.00 12200.00 5.00 0.30673 12000.00 12200.00 10.00 0.30685 12000.00 12200.00 15.00 0.30695 12000.00 12200.00 20.00 0.30704 12000.00 12200.00 25.00 0.30711 12000.00 12200.00 30.00 0.30716 12000.00 12200.00 35.00 0.30719 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12046.32 12128.91 36.55 0.30753 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/214.ctf NX, NY, NZ: 360 360 1 MODE : real