CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/208.mrc Output diagnostic file name Micrographs/208.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/208.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2438.000 7796.000 Mean, RMS : 4433.516 212.9365 TITLE 1: emi2mrc: 208.emi Sun Jan 19 21:24:35 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 109.485, Y 682.996, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.05939 8200.00 8000.00 0.00 0.06416 8400.00 8000.00 0.00 0.06881 8600.00 8000.00 0.00 0.07345 8800.00 8000.00 0.00 0.07845 9000.00 8000.00 0.00 0.08399 9200.00 8000.00 0.00 0.09021 9400.00 8000.00 0.00 0.09721 9600.00 8000.00 0.00 0.10470 9800.00 8000.00 0.00 0.11232 10000.00 8000.00 0.00 0.12005 10200.00 8000.00 0.00 0.12788 10400.00 8000.00 0.00 0.13583 10600.00 8000.00 0.00 0.14412 10800.00 8000.00 0.00 0.15275 11000.00 8000.00 0.00 0.16137 11200.00 8000.00 0.00 0.16959 11400.00 8000.00 0.00 0.17678 11600.00 8000.00 0.00 0.18208 11800.00 8000.00 0.00 0.18500 12000.00 8000.00 0.00 0.18559 11600.00 8200.00 0.00 0.18692 11800.00 8200.00 0.00 0.18976 12000.00 8200.00 0.00 0.19022 11600.00 8400.00 0.00 0.19177 11800.00 8400.00 0.00 0.19452 12000.00 8400.00 0.00 0.19482 11600.00 8600.00 0.00 0.19720 11800.00 8600.00 0.00 0.19982 12000.00 8600.00 0.00 0.19992 11600.00 8800.00 0.00 0.20320 11800.00 8800.00 0.00 0.20566 11600.00 9000.00 0.00 0.20938 11800.00 9000.00 0.00 0.21164 11600.00 9200.00 0.00 0.21549 11800.00 9200.00 0.00 0.21752 11600.00 9400.00 0.00 0.22129 11800.00 9400.00 0.00 0.22307 11600.00 9600.00 0.00 0.22672 11800.00 9600.00 0.00 0.22821 11600.00 9800.00 0.00 0.23236 11800.00 9800.00 0.00 0.23350 11400.00 10000.00 0.00 0.23452 11600.00 10000.00 0.00 0.23852 11800.00 10000.00 0.00 0.23923 11400.00 10200.00 0.00 0.24114 11600.00 10200.00 0.00 0.24475 11800.00 10200.00 0.00 0.24496 11400.00 10400.00 0.00 0.24755 11600.00 10400.00 0.00 0.25068 11400.00 10600.00 0.00 0.25339 11600.00 10600.00 0.00 0.25596 11400.00 10800.00 0.00 0.25810 11600.00 10800.00 0.00 0.26011 11400.00 11000.00 0.00 0.26200 11600.00 11000.00 0.00 0.26343 11400.00 11200.00 0.00 0.26581 11600.00 11200.00 0.00 0.26652 11400.00 11400.00 0.00 0.26897 11400.00 11600.00 0.00 0.27040 11600.00 11400.00 70.00 0.27041 11600.00 11400.00 75.00 0.27044 11600.00 11400.00 80.00 0.27045 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11664.29 11432.73 74.50 0.27063 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/208.ctf NX, NY, NZ: 360 360 1 MODE : real