CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/203.mrc Output diagnostic file name Micrographs/203.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/203.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2412.000 8490.000 Mean, RMS : 4432.397 215.7350 TITLE 1: emi2mrc: 203.emi Sun Jan 19 21:20:41 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X 107.350, Y 684.032, Z -35.048, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.02580 8200.00 8000.00 0.00 0.02719 8400.00 8000.00 0.00 0.02868 8600.00 8000.00 0.00 0.03050 8800.00 8000.00 0.00 0.03278 9000.00 8000.00 0.00 0.03533 9200.00 8000.00 0.00 0.03798 9400.00 8000.00 0.00 0.04070 9600.00 8000.00 0.00 0.04360 9800.00 8000.00 0.00 0.04692 10000.00 8000.00 0.00 0.05091 10200.00 8000.00 0.00 0.05558 10400.00 8000.00 0.00 0.06068 10600.00 8000.00 0.00 0.06600 10800.00 8000.00 0.00 0.07141 11000.00 8000.00 0.00 0.07695 11200.00 8000.00 0.00 0.08283 11400.00 8000.00 0.00 0.08911 11600.00 8000.00 0.00 0.09554 11800.00 8000.00 0.00 0.10181 12000.00 8000.00 0.00 0.10776 12200.00 8000.00 0.00 0.11350 12400.00 8000.00 0.00 0.11948 12600.00 8000.00 0.00 0.12614 12800.00 8000.00 0.00 0.13335 13000.00 8000.00 0.00 0.14043 13200.00 8000.00 0.00 0.14638 13400.00 8000.00 0.00 0.15031 13600.00 8000.00 0.00 0.15203 13800.00 8000.00 0.00 0.15206 13400.00 8200.00 0.00 0.15319 13600.00 8200.00 0.00 0.15483 13400.00 8400.00 0.00 0.15614 13600.00 8400.00 0.00 0.15771 13400.00 8600.00 0.00 0.15963 13600.00 8600.00 0.00 0.16113 13400.00 8800.00 0.00 0.16365 13600.00 8800.00 0.00 0.16507 13400.00 9000.00 0.00 0.16789 13600.00 9000.00 0.00 0.16921 13400.00 9200.00 0.00 0.17219 13600.00 9200.00 0.00 0.17342 13400.00 9400.00 0.00 0.17644 13600.00 9400.00 0.00 0.17754 13400.00 9600.00 0.00 0.18062 13600.00 9600.00 0.00 0.18159 13200.00 9800.00 0.00 0.18163 13400.00 9800.00 0.00 0.18517 13600.00 9800.00 0.00 0.18596 13200.00 10000.00 0.00 0.18683 13400.00 10000.00 0.00 0.19024 13600.00 10000.00 0.00 0.19086 13200.00 10200.00 0.00 0.19225 13400.00 10200.00 0.00 0.19553 13600.00 10200.00 0.00 0.19594 13200.00 10400.00 0.00 0.19767 13400.00 10400.00 0.00 0.20080 13600.00 10400.00 0.00 0.20099 13200.00 10600.00 0.00 0.20301 13400.00 10600.00 0.00 0.20599 13200.00 10800.00 0.00 0.20828 13400.00 10800.00 0.00 0.21110 13200.00 11000.00 0.00 0.21390 13400.00 11000.00 0.00 0.21654 13200.00 11200.00 0.00 0.22031 13400.00 11200.00 0.00 0.22272 13200.00 11400.00 0.00 0.22717 13400.00 11400.00 0.00 0.22931 13200.00 11600.00 0.00 0.23391 13400.00 11600.00 0.00 0.23574 13200.00 11800.00 0.00 0.24024 13400.00 11800.00 0.00 0.24172 13200.00 12000.00 0.00 0.24593 13400.00 12000.00 0.00 0.24704 13200.00 12200.00 0.00 0.25131 13400.00 12200.00 0.00 0.25201 13000.00 12400.00 0.00 0.25279 13200.00 12400.00 0.00 0.25724 13400.00 12400.00 0.00 0.25740 13000.00 12600.00 0.00 0.25968 13200.00 12600.00 0.00 0.26360 13000.00 12800.00 0.00 0.26605 13200.00 12800.00 0.00 0.26923 13000.00 13000.00 0.00 0.27091 13200.00 13000.00 0.00 0.27314 13000.00 13200.00 0.00 0.27336 13200.00 13200.00 0.00 0.27440 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13130.32 13205.54 23.38 0.27467 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/203.ctf NX, NY, NZ: 360 360 1 MODE : real