CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/183.mrc Output diagnostic file name Micrographs/183.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/183.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2562.000 8158.000 Mean, RMS : 4508.109 214.9474 TITLE 1: emi2mrc: 183.emi Sun Jan 19 21:02:44 2014 TITLE 2: kV 300, Mag 78000x, Def 14600, Spot 4, Int 55.11%, Obj 88.06%, Dif 73.67% TITLE 3: X 107.247, Y 688.234, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.02571 8200.00 8000.00 0.00 0.02737 8400.00 8000.00 0.00 0.02894 8600.00 8000.00 0.00 0.03070 8800.00 8000.00 0.00 0.03289 9000.00 8000.00 0.00 0.03554 9200.00 8000.00 0.00 0.03847 9400.00 8000.00 0.00 0.04154 9600.00 8000.00 0.00 0.04460 9800.00 8000.00 0.00 0.04772 10000.00 8000.00 0.00 0.05119 10200.00 8000.00 0.00 0.05521 10400.00 8000.00 0.00 0.05976 10600.00 8000.00 0.00 0.06475 10800.00 8000.00 0.00 0.06999 11000.00 8000.00 0.00 0.07537 11200.00 8000.00 0.00 0.08097 11400.00 8000.00 0.00 0.08694 11600.00 8000.00 0.00 0.09321 11800.00 8000.00 0.00 0.09961 12000.00 8000.00 0.00 0.10603 12200.00 8000.00 0.00 0.11238 12400.00 8000.00 0.00 0.11887 12600.00 8000.00 0.00 0.12580 12800.00 8000.00 0.00 0.13314 13000.00 8000.00 0.00 0.14045 13200.00 8000.00 0.00 0.14701 13400.00 8000.00 0.00 0.15200 13600.00 8000.00 0.00 0.15502 13800.00 8000.00 0.00 0.15631 14000.00 8000.00 0.00 0.15639 13600.00 8200.00 0.00 0.15769 13800.00 8200.00 0.00 0.15893 14000.00 8200.00 0.00 0.15894 13600.00 8400.00 0.00 0.16040 13800.00 8400.00 0.00 0.16158 13600.00 8600.00 0.00 0.16359 13800.00 8600.00 0.00 0.16471 13600.00 8800.00 0.00 0.16725 13800.00 8800.00 0.00 0.16832 13600.00 9000.00 0.00 0.17107 13800.00 9000.00 0.00 0.17207 13400.00 9200.00 0.00 0.17213 13600.00 9200.00 0.00 0.17491 13800.00 9200.00 0.00 0.17584 13400.00 9400.00 0.00 0.17591 13600.00 9400.00 0.00 0.17863 13800.00 9400.00 0.00 0.17947 13400.00 9600.00 0.00 0.17961 13600.00 9600.00 0.00 0.18225 13800.00 9600.00 0.00 0.18297 13400.00 9800.00 0.00 0.18365 13600.00 9800.00 0.00 0.18617 13800.00 9800.00 0.00 0.18676 13400.00 10000.00 0.00 0.18821 13600.00 10000.00 0.00 0.19061 13800.00 10000.00 0.00 0.19103 13400.00 10200.00 0.00 0.19296 13600.00 10200.00 0.00 0.19521 13800.00 10200.00 0.00 0.19546 13400.00 10400.00 0.00 0.19757 13600.00 10400.00 0.00 0.19968 13800.00 10400.00 0.00 0.19976 13400.00 10600.00 0.00 0.20189 13600.00 10600.00 0.00 0.20388 13400.00 10800.00 0.00 0.20584 13600.00 10800.00 0.00 0.20772 13400.00 11000.00 0.00 0.20987 13600.00 11000.00 0.00 0.21164 13400.00 11200.00 0.00 0.21449 13600.00 11200.00 0.00 0.21613 13400.00 11400.00 0.00 0.21957 13600.00 11400.00 0.00 0.22103 13400.00 11600.00 0.00 0.22466 13600.00 11600.00 0.00 0.22589 13400.00 11800.00 0.00 0.22945 13600.00 11800.00 0.00 0.23041 13400.00 12000.00 0.00 0.23360 13600.00 12000.00 0.00 0.23429 13400.00 12200.00 0.00 0.23733 13600.00 12200.00 0.00 0.23771 13200.00 12400.00 0.00 0.23801 13400.00 12400.00 0.00 0.24148 13200.00 12600.00 0.00 0.24316 13400.00 12600.00 0.00 0.24621 13200.00 12800.00 0.00 0.24846 13400.00 12800.00 0.00 0.25091 13200.00 13000.00 0.00 0.25335 13400.00 13000.00 0.00 0.25498 13200.00 13200.00 0.00 0.25695 13400.00 13200.00 0.00 0.25752 13200.00 13400.00 0.00 0.25817 13200.00 13400.00 5.00 0.25823 13200.00 13400.00 10.00 0.25828 13200.00 13400.00 15.00 0.25832 13200.00 13400.00 20.00 0.25834 13200.00 13400.00 25.00 0.25835 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13273.32 13363.33 15.78 0.25856 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/183.ctf NX, NY, NZ: 360 360 1 MODE : real