CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/182.mrc Output diagnostic file name Micrographs/182.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/182.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2392.000 7714.000 Mean, RMS : 4506.380 221.6976 TITLE 1: emi2mrc: 182.emi Sun Jan 19 21:02:10 2014 TITLE 2: kV 300, Mag 78000x, Def 14600, Spot 4, Int 55.11%, Obj 88.06%, Dif 73.67% TITLE 3: X 105.773, Y 686.365, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03052 8200.00 8000.00 0.00 0.03260 8400.00 8000.00 0.00 0.03471 8600.00 8000.00 0.00 0.03687 8800.00 8000.00 0.00 0.03918 9000.00 8000.00 0.00 0.04163 9200.00 8000.00 0.00 0.04433 9400.00 8000.00 0.00 0.04742 9600.00 8000.00 0.00 0.05093 9800.00 8000.00 0.00 0.05483 10000.00 8000.00 0.00 0.05915 10200.00 8000.00 0.00 0.06387 10400.00 8000.00 0.00 0.06895 10600.00 8000.00 0.00 0.07449 10800.00 8000.00 0.00 0.08049 11000.00 8000.00 0.00 0.08685 11200.00 8000.00 0.00 0.09350 11400.00 8000.00 0.00 0.10034 11600.00 8000.00 0.00 0.10723 11800.00 8000.00 0.00 0.11422 12000.00 8000.00 0.00 0.12153 12200.00 8000.00 0.00 0.12923 12400.00 8000.00 0.00 0.13724 12600.00 8000.00 0.00 0.14514 12800.00 8000.00 0.00 0.15209 13000.00 8000.00 0.00 0.15720 13200.00 8000.00 0.00 0.15994 13400.00 8000.00 0.00 0.16037 13000.00 8200.00 0.00 0.16038 13200.00 8200.00 0.00 0.16304 13400.00 8200.00 0.00 0.16335 13000.00 8400.00 0.00 0.16372 13200.00 8400.00 0.00 0.16630 13400.00 8400.00 0.00 0.16652 13000.00 8600.00 0.00 0.16768 13200.00 8600.00 0.00 0.17020 13400.00 8600.00 0.00 0.17033 13000.00 8800.00 0.00 0.17228 13200.00 8800.00 0.00 0.17474 13400.00 8800.00 0.00 0.17477 13000.00 9000.00 0.00 0.17721 13200.00 9000.00 0.00 0.17961 13000.00 9200.00 0.00 0.18234 13200.00 9200.00 0.00 0.18469 13000.00 9400.00 0.00 0.18750 13200.00 9400.00 0.00 0.18980 13000.00 9600.00 0.00 0.19265 13200.00 9600.00 0.00 0.19488 13000.00 9800.00 0.00 0.19817 13200.00 9800.00 0.00 0.20032 13000.00 10000.00 0.00 0.20430 13200.00 10000.00 0.00 0.20635 13000.00 10200.00 0.00 0.21080 13200.00 10200.00 0.00 0.21272 13000.00 10400.00 0.00 0.21754 13200.00 10400.00 0.00 0.21930 12800.00 10600.00 0.00 0.21967 13000.00 10600.00 0.00 0.22443 13200.00 10600.00 0.00 0.22601 12800.00 10800.00 0.00 0.22667 13000.00 10800.00 0.00 0.23133 13200.00 10800.00 0.00 0.23271 12800.00 11000.00 0.00 0.23396 13000.00 11000.00 0.00 0.23851 13200.00 11000.00 0.00 0.23966 12800.00 11200.00 0.00 0.24195 13000.00 11200.00 0.00 0.24634 13200.00 11200.00 0.00 0.24720 12800.00 11400.00 0.00 0.25035 13000.00 11400.00 0.00 0.25453 13200.00 11400.00 0.00 0.25505 12800.00 11600.00 0.00 0.25884 13000.00 11600.00 0.00 0.26276 13200.00 11600.00 0.00 0.26284 12800.00 11800.00 0.00 0.26741 13000.00 11800.00 0.00 0.27094 12800.00 12000.00 0.00 0.27572 13000.00 12000.00 0.00 0.27873 12800.00 12200.00 0.00 0.28362 13000.00 12200.00 0.00 0.28596 12800.00 12400.00 0.00 0.29136 13000.00 12400.00 0.00 0.29279 12600.00 12600.00 0.00 0.29322 12800.00 12600.00 0.00 0.29806 13000.00 12600.00 0.00 0.29827 12800.00 12800.00 0.00 0.30177 12800.00 13000.00 15.00 0.30181 12800.00 13000.00 20.00 0.30188 12800.00 13000.00 25.00 0.30193 12800.00 13000.00 30.00 0.30194 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12797.92 12910.51 31.02 0.30235 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/182.ctf NX, NY, NZ: 360 360 1 MODE : real