CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/171.mrc Output diagnostic file name Micrographs/171.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/171.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2330.000 7766.000 Mean, RMS : 4470.979 217.2844 TITLE 1: emi2mrc: 171.emi Sun Jan 19 20:51:26 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 116.730, Y 703.737, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.04392 8200.00 8000.00 0.00 0.04700 8400.00 8000.00 0.00 0.04965 8600.00 8000.00 0.00 0.05211 8800.00 8000.00 0.00 0.05489 9000.00 8000.00 0.00 0.05822 9200.00 8000.00 0.00 0.06214 9400.00 8000.00 0.00 0.06651 9600.00 8000.00 0.00 0.07096 9800.00 8000.00 0.00 0.07535 10000.00 8000.00 0.00 0.08000 10200.00 8000.00 0.00 0.08523 10400.00 8000.00 0.00 0.09120 10600.00 8000.00 0.00 0.09785 10800.00 8000.00 0.00 0.10481 11000.00 8000.00 0.00 0.11166 11200.00 8000.00 0.00 0.11843 11400.00 8000.00 0.00 0.12547 11600.00 8000.00 0.00 0.13306 11800.00 8000.00 0.00 0.14127 12000.00 8000.00 0.00 0.14972 12200.00 8000.00 0.00 0.15748 12400.00 8000.00 0.00 0.16368 12600.00 8000.00 0.00 0.16787 12800.00 8000.00 0.00 0.16994 13000.00 8000.00 0.00 0.17022 12600.00 8200.00 0.00 0.17171 12800.00 8200.00 0.00 0.17370 13000.00 8200.00 0.00 0.17387 12600.00 8400.00 0.00 0.17556 12800.00 8400.00 0.00 0.17745 13000.00 8400.00 0.00 0.17750 12600.00 8600.00 0.00 0.17986 12800.00 8600.00 0.00 0.18164 12600.00 8800.00 0.00 0.18462 12800.00 8800.00 0.00 0.18625 12600.00 9000.00 0.00 0.18953 12800.00 9000.00 0.00 0.19099 12600.00 9200.00 0.00 0.19444 12800.00 9200.00 0.00 0.19572 12600.00 9400.00 0.00 0.19924 12800.00 9400.00 0.00 0.20036 12400.00 9600.00 0.00 0.20056 12600.00 9600.00 0.00 0.20399 12800.00 9600.00 0.00 0.20495 12400.00 9800.00 0.00 0.20595 12600.00 9800.00 0.00 0.20926 12800.00 9800.00 0.00 0.21005 12400.00 10000.00 0.00 0.21218 12600.00 10000.00 0.00 0.21536 12800.00 10000.00 0.00 0.21596 12400.00 10200.00 0.00 0.21896 12600.00 10200.00 0.00 0.22197 12800.00 10200.00 0.00 0.22235 12400.00 10400.00 0.00 0.22591 12600.00 10400.00 0.00 0.22873 12800.00 10400.00 0.00 0.22885 12400.00 10600.00 0.00 0.23272 12600.00 10600.00 0.00 0.23531 12400.00 10800.00 0.00 0.23905 12600.00 10800.00 0.00 0.24141 12400.00 11000.00 0.00 0.24535 12600.00 11000.00 0.00 0.24741 12400.00 11200.00 0.00 0.25226 12600.00 11200.00 0.00 0.25392 12200.00 11400.00 0.00 0.25521 12400.00 11400.00 0.00 0.25964 12600.00 11400.00 0.00 0.26075 12200.00 11600.00 0.00 0.26314 12400.00 11600.00 0.00 0.26692 12600.00 11600.00 0.00 0.26735 12200.00 11800.00 0.00 0.27070 12400.00 11800.00 0.00 0.27362 12200.00 12000.00 0.00 0.27675 12400.00 12000.00 0.00 0.27868 12200.00 12200.00 0.00 0.28047 12400.00 12200.00 0.00 0.28151 12200.00 12400.00 0.00 0.28251 12400.00 12400.00 0.00 0.28284 12200.00 12600.00 0.00 0.28338 12200.00 12600.00 5.00 0.28347 12200.00 12600.00 10.00 0.28349 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12273.50 12573.47 5.39 0.28365 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/171.ctf NX, NY, NZ: 360 360 1 MODE : real