CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/166.mrc Output diagnostic file name Micrographs/166.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/166.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2114.000 7752.000 Mean, RMS : 4476.469 218.7155 TITLE 1: emi2mrc: 166.emi Sun Jan 19 20:43:57 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 106.709, Y 695.824, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.01293 8200.00 8000.00 0.00 0.01391 8400.00 8000.00 0.00 0.01484 8600.00 8000.00 0.00 0.01585 8800.00 8000.00 0.00 0.01706 9000.00 8000.00 0.00 0.01847 9200.00 8000.00 0.00 0.02004 9400.00 8000.00 0.00 0.02171 9600.00 8000.00 0.00 0.02345 9800.00 8000.00 0.00 0.02530 10000.00 8000.00 0.00 0.02741 10200.00 8000.00 0.00 0.02981 10400.00 8000.00 0.00 0.03243 10600.00 8000.00 0.00 0.03518 10800.00 8000.00 0.00 0.03796 11000.00 8000.00 0.00 0.04078 11200.00 8000.00 0.00 0.04381 11400.00 8000.00 0.00 0.04724 11600.00 8000.00 0.00 0.05108 11800.00 8000.00 0.00 0.05525 12000.00 8000.00 0.00 0.05959 12200.00 8000.00 0.00 0.06399 12400.00 8000.00 0.00 0.06850 12600.00 8000.00 0.00 0.07331 12800.00 8000.00 0.00 0.07848 13000.00 8000.00 0.00 0.08391 13200.00 8000.00 0.00 0.08942 13400.00 8000.00 0.00 0.09482 13600.00 8000.00 0.00 0.10017 13800.00 8000.00 0.00 0.10579 14000.00 8000.00 0.00 0.11194 14200.00 8000.00 0.00 0.11857 14400.00 8000.00 0.00 0.12524 14600.00 8000.00 0.00 0.13118 14800.00 8000.00 0.00 0.13562 15000.00 8000.00 0.00 0.13827 15200.00 8000.00 0.00 0.13929 15000.00 8200.00 0.00 0.14021 15200.00 8200.00 0.00 0.14121 15000.00 8400.00 0.00 0.14213 15200.00 8400.00 0.00 0.14313 15000.00 8600.00 0.00 0.14443 15200.00 8600.00 0.00 0.14541 15000.00 8800.00 0.00 0.14716 15200.00 8800.00 0.00 0.14811 15000.00 9000.00 0.00 0.15011 15200.00 9000.00 0.00 0.15102 15000.00 9200.00 0.00 0.15317 15200.00 9200.00 0.00 0.15402 15000.00 9400.00 0.00 0.15621 15200.00 9400.00 0.00 0.15698 15000.00 9600.00 0.00 0.15921 15200.00 9600.00 0.00 0.15992 14800.00 9800.00 0.00 0.16008 15000.00 9800.00 0.00 0.16252 15200.00 9800.00 0.00 0.16315 14800.00 10000.00 0.00 0.16384 15000.00 10000.00 0.00 0.16622 15200.00 10000.00 0.00 0.16679 14800.00 10200.00 0.00 0.16771 15000.00 10200.00 0.00 0.17003 15200.00 10200.00 0.00 0.17052 14800.00 10400.00 0.00 0.17147 15000.00 10400.00 0.00 0.17374 15200.00 10400.00 0.00 0.17415 14800.00 10600.00 0.00 0.17503 15000.00 10600.00 0.00 0.17725 15200.00 10600.00 0.00 0.17759 14800.00 10800.00 0.00 0.17838 15000.00 10800.00 0.00 0.18054 15200.00 10800.00 0.00 0.18081 14800.00 11000.00 0.00 0.18192 15000.00 11000.00 0.00 0.18402 15200.00 11000.00 0.00 0.18421 14800.00 11200.00 0.00 0.18611 15000.00 11200.00 0.00 0.18815 15200.00 11200.00 0.00 0.18824 14800.00 11400.00 0.00 0.19086 15000.00 11400.00 0.00 0.19281 14800.00 11600.00 0.00 0.19582 15000.00 11600.00 0.00 0.19766 14800.00 11800.00 0.00 0.20074 15000.00 11800.00 0.00 0.20248 14800.00 12000.00 0.00 0.20541 15000.00 12000.00 0.00 0.20703 14800.00 12200.00 0.00 0.20995 15000.00 12200.00 0.00 0.21143 14800.00 12400.00 0.00 0.21490 15000.00 12400.00 0.00 0.21620 14600.00 12600.00 0.00 0.21646 14800.00 12600.00 0.00 0.22037 15000.00 12600.00 0.00 0.22144 14600.00 12800.00 0.00 0.22226 14800.00 12800.00 0.00 0.22599 15000.00 12800.00 0.00 0.22676 14600.00 13000.00 0.00 0.22794 14800.00 13000.00 0.00 0.23143 15000.00 13000.00 0.00 0.23188 14600.00 13200.00 0.00 0.23317 14800.00 13200.00 0.00 0.23641 15000.00 13200.00 0.00 0.23651 14600.00 13400.00 0.00 0.23795 14800.00 13400.00 0.00 0.24094 14600.00 13600.00 0.00 0.24309 14800.00 13600.00 0.00 0.24575 14600.00 13800.00 0.00 0.24920 14800.00 13800.00 0.00 0.25138 14600.00 14000.00 0.00 0.25588 14800.00 14000.00 0.00 0.25738 14400.00 14200.00 0.00 0.25741 14600.00 14200.00 0.00 0.26232 14800.00 14200.00 0.00 0.26292 14400.00 14400.00 0.00 0.26357 14600.00 14400.00 0.00 0.26751 14600.00 14600.00 0.00 0.26995 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14565.79 14684.58 33.53 0.27020 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/166.ctf NX, NY, NZ: 360 360 1 MODE : real