CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/164.mrc Output diagnostic file name Micrographs/164.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/164.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2244.000 8104.000 Mean, RMS : 4477.723 217.0783 TITLE 1: emi2mrc: 164.emi Sun Jan 19 20:42:24 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.11%, Obj 88.08%, Dif 73.67% TITLE 3: X 103.199, Y 691.434, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.06111 8200.00 8000.00 0.00 0.06571 8400.00 8000.00 0.00 0.07031 8600.00 8000.00 0.00 0.07494 8800.00 8000.00 0.00 0.07991 9000.00 8000.00 0.00 0.08537 9200.00 8000.00 0.00 0.09151 9400.00 8000.00 0.00 0.09850 9600.00 8000.00 0.00 0.10607 9800.00 8000.00 0.00 0.11392 10000.00 8000.00 0.00 0.12205 10200.00 8000.00 0.00 0.13046 10400.00 8000.00 0.00 0.13923 10600.00 8000.00 0.00 0.14865 10800.00 8000.00 0.00 0.15866 11000.00 8000.00 0.00 0.16880 11200.00 8000.00 0.00 0.17843 11400.00 8000.00 0.00 0.18671 11600.00 8000.00 0.00 0.19262 11800.00 8000.00 0.00 0.19564 12000.00 8000.00 0.00 0.19593 11600.00 8200.00 0.00 0.19757 11800.00 8200.00 0.00 0.20041 12000.00 8200.00 0.00 0.20046 11600.00 8400.00 0.00 0.20252 11800.00 8400.00 0.00 0.20516 11600.00 8600.00 0.00 0.20800 11800.00 8600.00 0.00 0.21042 11600.00 8800.00 0.00 0.21410 11800.00 8800.00 0.00 0.21630 11600.00 9000.00 0.00 0.22051 11800.00 9000.00 0.00 0.22248 11600.00 9200.00 0.00 0.22713 11800.00 9200.00 0.00 0.22886 11600.00 9400.00 0.00 0.23369 11800.00 9400.00 0.00 0.23517 11400.00 9600.00 0.00 0.23534 11600.00 9600.00 0.00 0.24008 11800.00 9600.00 0.00 0.24128 11400.00 9800.00 0.00 0.24231 11600.00 9800.00 0.00 0.24681 11800.00 9800.00 0.00 0.24768 11400.00 10000.00 0.00 0.25002 11600.00 10000.00 0.00 0.25421 11800.00 10000.00 0.00 0.25469 11400.00 10200.00 0.00 0.25813 11600.00 10200.00 0.00 0.26194 11800.00 10200.00 0.00 0.26195 11400.00 10400.00 0.00 0.26643 11600.00 10400.00 0.00 0.26976 11400.00 10600.00 0.00 0.27456 11600.00 10600.00 0.00 0.27732 11400.00 10800.00 0.00 0.28171 11600.00 10800.00 0.00 0.28384 11400.00 11000.00 0.00 0.28775 11600.00 11000.00 0.00 0.28917 11400.00 11200.00 0.00 0.29293 11600.00 11200.00 0.00 0.29346 11400.00 11400.00 0.00 0.29627 11400.00 11600.00 0.00 0.29653 11400.00 11600.00 5.00 0.29660 11400.00 11600.00 10.00 0.29665 11400.00 11600.00 15.00 0.29668 11400.00 11600.00 20.00 0.29670 11400.00 11600.00 25.00 0.29671 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11424.07 11512.79 27.47 0.29702 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/164.ctf NX, NY, NZ: 360 360 1 MODE : real