CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/159.mrc Output diagnostic file name Micrographs/159.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/159.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2394.000 7970.000 Mean, RMS : 4369.936 219.7706 TITLE 1: emi2mrc: 159.emi Sun Jan 19 19:10:32 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 113.921, Y 708.993, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.01576 8200.00 8000.00 0.00 0.01680 8400.00 8000.00 0.00 0.01796 8600.00 8000.00 0.00 0.01931 8800.00 8000.00 0.00 0.02084 9000.00 8000.00 0.00 0.02243 9200.00 8000.00 0.00 0.02401 9400.00 8000.00 0.00 0.02562 9600.00 8000.00 0.00 0.02737 9800.00 8000.00 0.00 0.02940 10000.00 8000.00 0.00 0.03186 10200.00 8000.00 0.00 0.03474 10400.00 8000.00 0.00 0.03795 10600.00 8000.00 0.00 0.04140 10800.00 8000.00 0.00 0.04503 11000.00 8000.00 0.00 0.04879 11200.00 8000.00 0.00 0.05277 11400.00 8000.00 0.00 0.05699 11600.00 8000.00 0.00 0.06140 11800.00 8000.00 0.00 0.06598 12000.00 8000.00 0.00 0.07070 12200.00 8000.00 0.00 0.07553 12400.00 8000.00 0.00 0.08050 12600.00 8000.00 0.00 0.08564 12800.00 8000.00 0.00 0.09089 13000.00 8000.00 0.00 0.09621 13200.00 8000.00 0.00 0.10166 13400.00 8000.00 0.00 0.10729 13600.00 8000.00 0.00 0.11313 13800.00 8000.00 0.00 0.11908 14000.00 8000.00 0.00 0.12472 14200.00 8000.00 0.00 0.12946 14400.00 8000.00 0.00 0.13284 14600.00 8000.00 0.00 0.13469 14800.00 8000.00 0.00 0.13523 14400.00 8200.00 0.00 0.13524 14600.00 8200.00 0.00 0.13710 14800.00 8200.00 0.00 0.13763 14400.00 8400.00 0.00 0.13765 14600.00 8400.00 0.00 0.13951 14800.00 8400.00 0.00 0.13998 14400.00 8600.00 0.00 0.14035 14600.00 8600.00 0.00 0.14218 14800.00 8600.00 0.00 0.14259 14400.00 8800.00 0.00 0.14333 14600.00 8800.00 0.00 0.14512 14800.00 8800.00 0.00 0.14545 14400.00 9000.00 0.00 0.14644 14600.00 9000.00 0.00 0.14817 14800.00 9000.00 0.00 0.14842 14400.00 9200.00 0.00 0.14969 14600.00 9200.00 0.00 0.15137 14800.00 9200.00 0.00 0.15153 14400.00 9400.00 0.00 0.15306 14600.00 9400.00 0.00 0.15468 14800.00 9400.00 0.00 0.15474 14400.00 9600.00 0.00 0.15655 14600.00 9600.00 0.00 0.15810 14400.00 9800.00 0.00 0.16041 14600.00 9800.00 0.00 0.16187 14400.00 10000.00 0.00 0.16466 14600.00 10000.00 0.00 0.16603 14400.00 10200.00 0.00 0.16901 14600.00 10200.00 0.00 0.17027 14400.00 10400.00 0.00 0.17332 14600.00 10400.00 0.00 0.17449 14200.00 10600.00 0.00 0.17455 14400.00 10600.00 0.00 0.17766 14600.00 10600.00 0.00 0.17873 14200.00 10800.00 0.00 0.17900 14400.00 10800.00 0.00 0.18205 14600.00 10800.00 0.00 0.18302 14200.00 11000.00 0.00 0.18383 14400.00 11000.00 0.00 0.18681 14600.00 11000.00 0.00 0.18768 14200.00 11200.00 0.00 0.18931 14400.00 11200.00 0.00 0.19222 14600.00 11200.00 0.00 0.19297 14200.00 11400.00 0.00 0.19519 14400.00 11400.00 0.00 0.19802 14600.00 11400.00 0.00 0.19865 14200.00 11600.00 0.00 0.20113 14400.00 11600.00 0.00 0.20388 14600.00 11600.00 0.00 0.20438 14200.00 11800.00 0.00 0.20705 14400.00 11800.00 0.00 0.20971 14600.00 11800.00 0.00 0.21007 14200.00 12000.00 0.00 0.21289 14400.00 12000.00 0.00 0.21547 14600.00 12000.00 0.00 0.21568 14200.00 12200.00 0.00 0.21885 14400.00 12200.00 0.00 0.22133 14600.00 12200.00 0.00 0.22139 14200.00 12400.00 0.00 0.22538 14400.00 12400.00 0.00 0.22774 14000.00 12600.00 0.00 0.22783 14200.00 12600.00 0.00 0.23241 14400.00 12600.00 0.00 0.23461 14000.00 12800.00 0.00 0.23495 14200.00 12800.00 0.00 0.23944 14400.00 12800.00 0.00 0.24145 14000.00 13000.00 0.00 0.24196 14200.00 13000.00 0.00 0.24632 14400.00 13000.00 0.00 0.24809 14000.00 13200.00 0.00 0.24876 14200.00 13200.00 0.00 0.25296 14400.00 13200.00 0.00 0.25441 14000.00 13400.00 0.00 0.25537 14200.00 13400.00 0.00 0.25935 14400.00 13400.00 0.00 0.26040 14000.00 13600.00 0.00 0.26234 14200.00 13600.00 0.00 0.26596 14400.00 13600.00 0.00 0.26646 14000.00 13800.00 0.00 0.26966 14200.00 13800.00 0.00 0.27265 14000.00 14000.00 0.00 0.27581 14200.00 14000.00 0.00 0.27787 14000.00 14200.00 0.00 0.27916 14200.00 14200.00 0.00 0.28013 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14142.70 14244.75 -3.97 0.28030 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/159.ctf NX, NY, NZ: 360 360 1 MODE : real