CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/142.mrc Output diagnostic file name Micrographs/142.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/142.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2322.000 8012.000 Mean, RMS : 4376.875 215.4358 TITLE 1: emi2mrc: 142.emi Sun Jan 19 19:00:11 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 95.739, Y 690.827, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.02401 8200.00 8000.00 0.00 0.02560 8400.00 8000.00 0.00 0.02700 8600.00 8000.00 0.00 0.02845 8800.00 8000.00 0.00 0.03021 9000.00 8000.00 0.00 0.03234 9200.00 8000.00 0.00 0.03481 9400.00 8000.00 0.00 0.03755 9600.00 8000.00 0.00 0.04044 9800.00 8000.00 0.00 0.04348 10000.00 8000.00 0.00 0.04685 10200.00 8000.00 0.00 0.05061 10400.00 8000.00 0.00 0.05466 10600.00 8000.00 0.00 0.05889 10800.00 8000.00 0.00 0.06314 11000.00 8000.00 0.00 0.06738 11200.00 8000.00 0.00 0.07181 11400.00 8000.00 0.00 0.07666 11600.00 8000.00 0.00 0.08194 11800.00 8000.00 0.00 0.08751 12000.00 8000.00 0.00 0.09313 12200.00 8000.00 0.00 0.09858 12400.00 8000.00 0.00 0.10393 12600.00 8000.00 0.00 0.10950 12800.00 8000.00 0.00 0.11550 13000.00 8000.00 0.00 0.12187 13200.00 8000.00 0.00 0.12825 13400.00 8000.00 0.00 0.13402 13600.00 8000.00 0.00 0.13865 13800.00 8000.00 0.00 0.14195 14000.00 8000.00 0.00 0.14400 14200.00 8000.00 0.00 0.14500 14400.00 8000.00 0.00 0.14529 14000.00 8200.00 0.00 0.14688 14200.00 8200.00 0.00 0.14780 14400.00 8200.00 0.00 0.14800 14000.00 8400.00 0.00 0.14971 14200.00 8400.00 0.00 0.15053 14400.00 8400.00 0.00 0.15064 13800.00 8600.00 0.00 0.15109 14000.00 8600.00 0.00 0.15287 14200.00 8600.00 0.00 0.15359 14400.00 8600.00 0.00 0.15359 13800.00 8800.00 0.00 0.15473 14000.00 8800.00 0.00 0.15641 14200.00 8800.00 0.00 0.15703 13800.00 9000.00 0.00 0.15857 14000.00 9000.00 0.00 0.16016 14200.00 9000.00 0.00 0.16070 13800.00 9200.00 0.00 0.16257 14000.00 9200.00 0.00 0.16409 14200.00 9200.00 0.00 0.16457 13800.00 9400.00 0.00 0.16662 14000.00 9400.00 0.00 0.16808 14200.00 9400.00 0.00 0.16851 13800.00 9600.00 0.00 0.17071 14000.00 9600.00 0.00 0.17211 14200.00 9600.00 0.00 0.17249 13600.00 9800.00 0.00 0.17253 13800.00 9800.00 0.00 0.17517 14000.00 9800.00 0.00 0.17651 14200.00 9800.00 0.00 0.17682 13600.00 10000.00 0.00 0.17757 13800.00 10000.00 0.00 0.18013 14000.00 10000.00 0.00 0.18139 14200.00 10000.00 0.00 0.18161 13600.00 10200.00 0.00 0.18279 13800.00 10200.00 0.00 0.18527 14000.00 10200.00 0.00 0.18644 14200.00 10200.00 0.00 0.18657 13600.00 10400.00 0.00 0.18796 13800.00 10400.00 0.00 0.19036 14000.00 10400.00 0.00 0.19145 14200.00 10400.00 0.00 0.19149 13600.00 10600.00 0.00 0.19299 13800.00 10600.00 0.00 0.19533 14000.00 10600.00 0.00 0.19635 13600.00 10800.00 0.00 0.19787 13800.00 10800.00 0.00 0.20016 14000.00 10800.00 0.00 0.20113 13600.00 11000.00 0.00 0.20302 13800.00 11000.00 0.00 0.20527 14000.00 11000.00 0.00 0.20616 13600.00 11200.00 0.00 0.20893 13800.00 11200.00 0.00 0.21110 14000.00 11200.00 0.00 0.21187 13600.00 11400.00 0.00 0.21539 13800.00 11400.00 0.00 0.21743 14000.00 11400.00 0.00 0.21803 13400.00 11600.00 0.00 0.21825 13600.00 11600.00 0.00 0.22190 13800.00 11600.00 0.00 0.22377 14000.00 11600.00 0.00 0.22416 13400.00 11800.00 0.00 0.22459 13600.00 11800.00 0.00 0.22811 13800.00 11800.00 0.00 0.22977 14000.00 11800.00 0.00 0.22990 13400.00 12000.00 0.00 0.23030 13600.00 12000.00 0.00 0.23368 13800.00 12000.00 0.00 0.23512 13400.00 12200.00 0.00 0.23563 13600.00 12200.00 0.00 0.23886 13800.00 12200.00 0.00 0.24004 13400.00 12400.00 0.00 0.24152 13600.00 12400.00 0.00 0.24450 13800.00 12400.00 0.00 0.24531 13400.00 12600.00 0.00 0.24828 13600.00 12600.00 0.00 0.25083 13800.00 12600.00 0.00 0.25111 13400.00 12800.00 0.00 0.25535 13600.00 12800.00 0.00 0.25727 13200.00 13000.00 0.00 0.25780 13400.00 13000.00 0.00 0.26208 13600.00 13000.00 0.00 0.26312 13200.00 13200.00 0.00 0.26405 13400.00 13200.00 0.00 0.26731 13200.00 13400.00 0.00 0.26766 13400.00 13400.00 0.00 0.26972 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13353.82 13489.54 28.47 0.27002 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/142.ctf NX, NY, NZ: 360 360 1 MODE : real