CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/132.mrc Output diagnostic file name Micrographs/132.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/132.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2156.000 7872.000 Mean, RMS : 4377.847 220.9480 TITLE 1: emi2mrc: 132.emi Sun Jan 19 18:51:59 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 106.515, Y 707.936, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00083 8200.00 8000.00 0.00 -0.00058 8400.00 8000.00 0.00 -0.00045 8600.00 8000.00 0.00 -0.00038 8800.00 8000.00 0.00 -0.00028 9000.00 8000.00 0.00 -0.00009 9200.00 8000.00 0.00 0.00024 9400.00 8000.00 0.00 0.00069 9600.00 8000.00 0.00 0.00123 9800.00 8000.00 0.00 0.00182 10000.00 8000.00 0.00 0.00246 10200.00 8000.00 0.00 0.00317 10400.00 8000.00 0.00 0.00399 10600.00 8000.00 0.00 0.00491 10800.00 8000.00 0.00 0.00591 11000.00 8000.00 0.00 0.00695 11200.00 8000.00 0.00 0.00806 11400.00 8000.00 0.00 0.00929 11600.00 8000.00 0.00 0.01067 11800.00 8000.00 0.00 0.01224 12000.00 8000.00 0.00 0.01395 12200.00 8000.00 0.00 0.01574 12400.00 8000.00 0.00 0.01754 12600.00 8000.00 0.00 0.01935 12800.00 8000.00 0.00 0.02118 13000.00 8000.00 0.00 0.02309 13200.00 8000.00 0.00 0.02511 13400.00 8000.00 0.00 0.02724 13600.00 8000.00 0.00 0.02945 13800.00 8000.00 0.00 0.03176 14000.00 8000.00 0.00 0.03419 14200.00 8000.00 0.00 0.03672 14400.00 8000.00 0.00 0.03935 14600.00 8000.00 0.00 0.04202 14800.00 8000.00 0.00 0.04467 15000.00 8000.00 0.00 0.04732 15200.00 8000.00 0.00 0.05003 15400.00 8000.00 0.00 0.05288 15600.00 8000.00 0.00 0.05593 15800.00 8000.00 0.00 0.05919 16000.00 8000.00 0.00 0.06257 16200.00 8000.00 0.00 0.06600 16400.00 8000.00 0.00 0.06950 16600.00 8000.00 0.00 0.07309 16800.00 8000.00 0.00 0.07687 17000.00 8000.00 0.00 0.08090 17200.00 8000.00 0.00 0.08504 17400.00 8000.00 0.00 0.08907 17600.00 8000.00 0.00 0.09270 17800.00 8000.00 0.00 0.09566 18000.00 8000.00 0.00 0.09778 18200.00 8000.00 0.00 0.09912 18400.00 8000.00 0.00 0.09986 18600.00 8000.00 0.00 0.10022 18800.00 8000.00 0.00 0.10043 19000.00 8000.00 0.00 0.10054 18400.00 8200.00 0.00 0.10111 18600.00 8200.00 0.00 0.10147 18800.00 8200.00 0.00 0.10166 19000.00 8200.00 0.00 0.10176 18400.00 8400.00 0.00 0.10229 18600.00 8400.00 0.00 0.10264 18800.00 8400.00 0.00 0.10283 19000.00 8400.00 0.00 0.10290 18400.00 8600.00 0.00 0.10358 18600.00 8600.00 0.00 0.10393 18800.00 8600.00 0.00 0.10409 19000.00 8600.00 0.00 0.10414 18200.00 8800.00 0.00 0.10431 18400.00 8800.00 0.00 0.10504 18600.00 8800.00 0.00 0.10537 18800.00 8800.00 0.00 0.10550 19000.00 8800.00 0.00 0.10552 18200.00 9000.00 0.00 0.10591 18400.00 9000.00 0.00 0.10663 18600.00 9000.00 0.00 0.10693 18800.00 9000.00 0.00 0.10703 18200.00 9200.00 0.00 0.10763 18400.00 9200.00 0.00 0.10834 18600.00 9200.00 0.00 0.10861 18800.00 9200.00 0.00 0.10866 18200.00 9400.00 0.00 0.10941 18400.00 9400.00 0.00 0.11010 18600.00 9400.00 0.00 0.11033 18800.00 9400.00 0.00 0.11035 18200.00 9600.00 0.00 0.11120 18400.00 9600.00 0.00 0.11185 18600.00 9600.00 0.00 0.11205 18200.00 9800.00 0.00 0.11308 18400.00 9800.00 0.00 0.11370 18600.00 9800.00 0.00 0.11385 18200.00 10000.00 0.00 0.11509 18400.00 10000.00 0.00 0.11567 18600.00 10000.00 0.00 0.11577 18000.00 10200.00 0.00 0.11588 18200.00 10200.00 0.00 0.11714 18400.00 10200.00 0.00 0.11768 18600.00 10200.00 0.00 0.11773 18000.00 10400.00 0.00 0.11800 18200.00 10400.00 0.00 0.11923 18400.00 10400.00 0.00 0.11973 18600.00 10400.00 0.00 0.11974 18000.00 10600.00 0.00 0.12019 18200.00 10600.00 0.00 0.12140 18400.00 10600.00 0.00 0.12187 18000.00 10800.00 0.00 0.12247 18200.00 10800.00 0.00 0.12366 18400.00 10800.00 0.00 0.12411 18000.00 11000.00 0.00 0.12497 18200.00 11000.00 0.00 0.12614 18400.00 11000.00 0.00 0.12655 18000.00 11200.00 0.00 0.12776 18200.00 11200.00 0.00 0.12889 18400.00 11200.00 0.00 0.12926 18000.00 11400.00 0.00 0.13067 18200.00 11400.00 0.00 0.13175 18400.00 11400.00 0.00 0.13205 18000.00 11600.00 0.00 0.13350 18200.00 11600.00 0.00 0.13452 18400.00 11600.00 0.00 0.13477 18000.00 11800.00 0.00 0.13621 18200.00 11800.00 0.00 0.13719 18400.00 11800.00 0.00 0.13740 18000.00 12000.00 0.00 0.13881 18200.00 12000.00 0.00 0.13976 18400.00 12000.00 0.00 0.13994 18000.00 12200.00 0.00 0.14149 18200.00 12200.00 0.00 0.14242 18400.00 12200.00 0.00 0.14258 17800.00 12400.00 0.00 0.14264 18000.00 12400.00 0.00 0.14456 18200.00 12400.00 0.00 0.14548 18400.00 12400.00 0.00 0.14561 17800.00 12600.00 0.00 0.14615 18000.00 12600.00 0.00 0.14807 18200.00 12600.00 0.00 0.14897 18400.00 12600.00 0.00 0.14906 17800.00 12800.00 0.00 0.14990 18000.00 12800.00 0.00 0.15181 18200.00 12800.00 0.00 0.15269 18400.00 12800.00 0.00 0.15274 17800.00 13000.00 0.00 0.15375 18000.00 13000.00 0.00 0.15564 18200.00 13000.00 0.00 0.15648 17800.00 13200.00 0.00 0.15751 18000.00 13200.00 0.00 0.15937 18200.00 13200.00 0.00 0.16015 17800.00 13400.00 0.00 0.16108 18000.00 13400.00 0.00 0.16289 18200.00 13400.00 0.00 0.16360 17800.00 13600.00 0.00 0.16471 18000.00 13600.00 0.00 0.16647 18200.00 13600.00 0.00 0.16710 17800.00 13800.00 0.00 0.16863 18000.00 13800.00 0.00 0.17032 18200.00 13800.00 0.00 0.17087 17800.00 14000.00 0.00 0.17274 18000.00 14000.00 0.00 0.17437 18200.00 14000.00 0.00 0.17484 17800.00 14200.00 0.00 0.17694 18000.00 14200.00 0.00 0.17851 18200.00 14200.00 0.00 0.17891 17800.00 14400.00 0.00 0.18116 18000.00 14400.00 0.00 0.18269 18200.00 14400.00 0.00 0.18302 17800.00 14600.00 0.00 0.18525 18000.00 14600.00 0.00 0.18673 18200.00 14600.00 0.00 0.18700 17800.00 14800.00 0.00 0.18935 18000.00 14800.00 0.00 0.19077 18200.00 14800.00 0.00 0.19095 17600.00 15000.00 0.00 0.19097 17800.00 15000.00 0.00 0.19373 18000.00 15000.00 0.00 0.19507 18200.00 15000.00 0.00 0.19514 17600.00 15200.00 0.00 0.19568 17800.00 15200.00 0.00 0.19838 18000.00 15200.00 0.00 0.19960 17600.00 15400.00 0.00 0.20050 17800.00 15400.00 0.00 0.20310 18000.00 15400.00 0.00 0.20418 17600.00 15600.00 0.00 0.20531 17800.00 15600.00 0.00 0.20780 18000.00 15600.00 0.00 0.20874 17600.00 15800.00 0.00 0.20993 17800.00 15800.00 0.00 0.21230 18000.00 15800.00 0.00 0.21308 17600.00 16000.00 0.00 0.21438 17800.00 16000.00 0.00 0.21665 18000.00 16000.00 0.00 0.21727 17600.00 16200.00 0.00 0.21919 17800.00 16200.00 0.00 0.22133 18000.00 16200.00 0.00 0.22176 17600.00 16400.00 0.00 0.22463 17800.00 16400.00 0.00 0.22659 18000.00 16400.00 0.00 0.22681 17400.00 16600.00 0.00 0.22727 17600.00 16600.00 0.00 0.23063 17800.00 16600.00 0.00 0.23235 17400.00 16800.00 0.00 0.23402 17600.00 16800.00 0.00 0.23708 17800.00 16800.00 0.00 0.23848 17400.00 17000.00 0.00 0.24080 17600.00 17000.00 0.00 0.24344 17800.00 17000.00 0.00 0.24439 17400.00 17200.00 0.00 0.24657 17600.00 17200.00 0.00 0.24871 17800.00 17200.00 0.00 0.24913 17400.00 17400.00 0.00 0.25077 17600.00 17400.00 0.00 0.25240 17400.00 17600.00 0.00 0.25325 17600.00 17600.00 0.00 0.25428 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17579.60 17669.88 25.21 0.25444 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/132.ctf NX, NY, NZ: 360 360 1 MODE : real