CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/131.mrc Output diagnostic file name Micrographs/131.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/131.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2312.000 7652.000 Mean, RMS : 4378.051 219.5344 TITLE 1: emi2mrc: 131.emi Sun Jan 19 18:50:43 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.13%, Obj 88.08%, Dif 73.67% TITLE 3: X 108.052, Y 710.021, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00744 8200.00 8000.00 0.00 -0.00695 8400.00 8000.00 0.00 -0.00640 8600.00 8000.00 0.00 -0.00586 8800.00 8000.00 0.00 -0.00536 9000.00 8000.00 0.00 -0.00490 9200.00 8000.00 0.00 -0.00448 9400.00 8000.00 0.00 -0.00407 9600.00 8000.00 0.00 -0.00363 9800.00 8000.00 0.00 -0.00316 10000.00 8000.00 0.00 -0.00264 10200.00 8000.00 0.00 -0.00210 10400.00 8000.00 0.00 -0.00153 10600.00 8000.00 0.00 -0.00092 10800.00 8000.00 0.00 -0.00022 11000.00 8000.00 0.00 0.00058 11200.00 8000.00 0.00 0.00152 11400.00 8000.00 0.00 0.00259 11600.00 8000.00 0.00 0.00382 11800.00 8000.00 0.00 0.00520 12000.00 8000.00 0.00 0.00673 12200.00 8000.00 0.00 0.00840 12400.00 8000.00 0.00 0.01015 12600.00 8000.00 0.00 0.01195 12800.00 8000.00 0.00 0.01374 13000.00 8000.00 0.00 0.01553 13200.00 8000.00 0.00 0.01735 13400.00 8000.00 0.00 0.01928 13600.00 8000.00 0.00 0.02140 13800.00 8000.00 0.00 0.02375 14000.00 8000.00 0.00 0.02630 14200.00 8000.00 0.00 0.02897 14400.00 8000.00 0.00 0.03168 14600.00 8000.00 0.00 0.03440 14800.00 8000.00 0.00 0.03712 15000.00 8000.00 0.00 0.03988 15200.00 8000.00 0.00 0.04274 15400.00 8000.00 0.00 0.04569 15600.00 8000.00 0.00 0.04872 15800.00 8000.00 0.00 0.05180 16000.00 8000.00 0.00 0.05490 16200.00 8000.00 0.00 0.05803 16400.00 8000.00 0.00 0.06125 16600.00 8000.00 0.00 0.06455 16800.00 8000.00 0.00 0.06794 17000.00 8000.00 0.00 0.07147 17200.00 8000.00 0.00 0.07513 17400.00 8000.00 0.00 0.07891 17600.00 8000.00 0.00 0.08273 17800.00 8000.00 0.00 0.08637 18000.00 8000.00 0.00 0.08953 18200.00 8000.00 0.00 0.09197 18400.00 8000.00 0.00 0.09356 18600.00 8000.00 0.00 0.09436 18800.00 8000.00 0.00 0.09463 18400.00 8200.00 0.00 0.09485 18600.00 8200.00 0.00 0.09569 18800.00 8200.00 0.00 0.09600 19000.00 8200.00 0.00 0.09602 18400.00 8400.00 0.00 0.09615 18600.00 8400.00 0.00 0.09704 18800.00 8400.00 0.00 0.09740 19000.00 8400.00 0.00 0.09745 18400.00 8600.00 0.00 0.09759 18600.00 8600.00 0.00 0.09852 18800.00 8600.00 0.00 0.09891 19000.00 8600.00 0.00 0.09899 18400.00 8800.00 0.00 0.09913 18600.00 8800.00 0.00 0.10011 18800.00 8800.00 0.00 0.10052 19000.00 8800.00 0.00 0.10061 18400.00 9000.00 0.00 0.10069 18600.00 9000.00 0.00 0.10170 18800.00 9000.00 0.00 0.10213 19000.00 9000.00 0.00 0.10220 18400.00 9200.00 0.00 0.10228 18600.00 9200.00 0.00 0.10331 18800.00 9200.00 0.00 0.10373 19000.00 9200.00 0.00 0.10377 18400.00 9400.00 0.00 0.10391 18600.00 9400.00 0.00 0.10494 18800.00 9400.00 0.00 0.10534 18400.00 9600.00 0.00 0.10561 18600.00 9600.00 0.00 0.10663 18800.00 9600.00 0.00 0.10698 18400.00 9800.00 0.00 0.10753 18600.00 9800.00 0.00 0.10851 18800.00 9800.00 0.00 0.10879 18400.00 10000.00 0.00 0.10967 18600.00 10000.00 0.00 0.11058 18800.00 10000.00 0.00 0.11079 18400.00 10200.00 0.00 0.11186 18600.00 10200.00 0.00 0.11270 18800.00 10200.00 0.00 0.11281 18400.00 10400.00 0.00 0.11399 18600.00 10400.00 0.00 0.11476 18800.00 10400.00 0.00 0.11479 18400.00 10600.00 0.00 0.11604 18600.00 10600.00 0.00 0.11672 18400.00 10800.00 0.00 0.11797 18600.00 10800.00 0.00 0.11860 18400.00 11000.00 0.00 0.11998 18600.00 11000.00 0.00 0.12055 18200.00 11200.00 0.00 0.12064 18400.00 11200.00 0.00 0.12220 18600.00 11200.00 0.00 0.12273 18200.00 11400.00 0.00 0.12302 18400.00 11400.00 0.00 0.12455 18600.00 11400.00 0.00 0.12506 18200.00 11600.00 0.00 0.12541 18400.00 11600.00 0.00 0.12694 18600.00 11600.00 0.00 0.12745 18200.00 11800.00 0.00 0.12780 18400.00 11800.00 0.00 0.12934 18600.00 11800.00 0.00 0.12986 18200.00 12000.00 0.00 0.13016 18400.00 12000.00 0.00 0.13173 18600.00 12000.00 0.00 0.13224 18200.00 12200.00 0.00 0.13259 18400.00 12200.00 0.00 0.13417 18600.00 12200.00 0.00 0.13468 18200.00 12400.00 0.00 0.13531 18400.00 12400.00 0.00 0.13689 18600.00 12400.00 0.00 0.13738 18200.00 12600.00 0.00 0.13830 18400.00 12600.00 0.00 0.13988 18600.00 12600.00 0.00 0.14034 18200.00 12800.00 0.00 0.14138 18400.00 12800.00 0.00 0.14296 18600.00 12800.00 0.00 0.14339 18200.00 13000.00 0.00 0.14450 18400.00 13000.00 0.00 0.14608 18600.00 13000.00 0.00 0.14647 18200.00 13200.00 0.00 0.14760 18400.00 13200.00 0.00 0.14916 18600.00 13200.00 0.00 0.14952 18200.00 13400.00 0.00 0.15068 18400.00 13400.00 0.00 0.15223 18600.00 13400.00 0.00 0.15254 18200.00 13600.00 0.00 0.15399 18400.00 13600.00 0.00 0.15552 18600.00 13600.00 0.00 0.15578 18200.00 13800.00 0.00 0.15767 18400.00 13800.00 0.00 0.15917 18600.00 13800.00 0.00 0.15938 18200.00 14000.00 0.00 0.16154 18400.00 14000.00 0.00 0.16302 18600.00 14000.00 0.00 0.16316 18200.00 14200.00 0.00 0.16549 18400.00 14200.00 0.00 0.16692 18600.00 14200.00 0.00 0.16699 18200.00 14400.00 0.00 0.16946 18400.00 14400.00 0.00 0.17084 18200.00 14600.00 0.00 0.17335 18400.00 14600.00 0.00 0.17466 18200.00 14800.00 0.00 0.17731 18400.00 14800.00 0.00 0.17853 18000.00 15000.00 0.00 0.17888 18200.00 15000.00 0.00 0.18156 18400.00 15000.00 0.00 0.18268 18000.00 15200.00 0.00 0.18340 18200.00 15200.00 0.00 0.18602 18400.00 15200.00 0.00 0.18703 18000.00 15400.00 0.00 0.18793 18200.00 15400.00 0.00 0.19048 18400.00 15400.00 0.00 0.19139 18000.00 15600.00 0.00 0.19246 18200.00 15600.00 0.00 0.19495 18400.00 15600.00 0.00 0.19575 18000.00 15800.00 0.00 0.19693 18200.00 15800.00 0.00 0.19937 18400.00 15800.00 0.00 0.20007 18000.00 16000.00 0.00 0.20148 18200.00 16000.00 0.00 0.20386 18400.00 16000.00 0.00 0.20445 18000.00 16200.00 0.00 0.20645 18200.00 16200.00 0.00 0.20876 18400.00 16200.00 0.00 0.20923 18000.00 16400.00 0.00 0.21190 18200.00 16400.00 0.00 0.21412 18400.00 16400.00 0.00 0.21442 18000.00 16600.00 0.00 0.21753 18200.00 16600.00 0.00 0.21964 18400.00 16600.00 0.00 0.21973 18000.00 16800.00 0.00 0.22328 18200.00 16800.00 0.00 0.22523 17800.00 17000.00 0.00 0.22546 18000.00 17000.00 0.00 0.22905 18200.00 17000.00 0.00 0.23078 17800.00 17200.00 0.00 0.23124 18000.00 17200.00 0.00 0.23470 18200.00 17200.00 0.00 0.23615 17800.00 17400.00 0.00 0.23720 18000.00 17400.00 0.00 0.24043 18200.00 17400.00 0.00 0.24149 17800.00 17600.00 0.00 0.24326 18000.00 17600.00 0.00 0.24606 18200.00 17600.00 0.00 0.24653 17800.00 17800.00 0.00 0.24827 18000.00 17800.00 0.00 0.25041 17800.00 18000.00 0.00 0.25097 18000.00 18000.00 0.00 0.25231 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17972.84 18060.89 33.67 0.25243 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/131.ctf NX, NY, NZ: 360 360 1 MODE : real