CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/107.mrc Output diagnostic file name Micrographs/107.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/107.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2330.000 8510.000 Mean, RMS : 4382.773 224.0498 TITLE 1: emi2mrc: 107.emi Sun Jan 19 18:25:12 2014 TITLE 2: kV 300, Mag 78000x, Def 22150, Spot 4, Int 55.13%, Obj 88.07%, Dif 73.67% TITLE 3: X 89.249, Y 699.559, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01934 8600.00 8000.00 0.00 -0.01928 10200.00 8000.00 0.00 -0.01907 10400.00 8000.00 0.00 -0.01875 10600.00 8000.00 0.00 -0.01837 10800.00 8000.00 0.00 -0.01798 11000.00 8000.00 0.00 -0.01764 11200.00 8000.00 0.00 -0.01742 11400.00 8000.00 0.00 -0.01728 11600.00 8000.00 0.00 -0.01713 11800.00 8000.00 0.00 -0.01687 12000.00 8000.00 0.00 -0.01647 12200.00 8000.00 0.00 -0.01595 12400.00 8000.00 0.00 -0.01538 12600.00 8000.00 0.00 -0.01483 12800.00 8000.00 0.00 -0.01431 13000.00 8000.00 0.00 -0.01376 13200.00 8000.00 0.00 -0.01312 13400.00 8000.00 0.00 -0.01236 13600.00 8000.00 0.00 -0.01147 13800.00 8000.00 0.00 -0.01047 14000.00 8000.00 0.00 -0.00942 14200.00 8000.00 0.00 -0.00836 14400.00 8000.00 0.00 -0.00731 14600.00 8000.00 0.00 -0.00628 14800.00 8000.00 0.00 -0.00524 15000.00 8000.00 0.00 -0.00414 15200.00 8000.00 0.00 -0.00290 15400.00 8000.00 0.00 -0.00147 15600.00 8000.00 0.00 0.00014 15800.00 8000.00 0.00 0.00191 16000.00 8000.00 0.00 0.00376 16200.00 8000.00 0.00 0.00565 16400.00 8000.00 0.00 0.00760 16600.00 8000.00 0.00 0.00962 16800.00 8000.00 0.00 0.01170 17000.00 8000.00 0.00 0.01383 17200.00 8000.00 0.00 0.01593 17400.00 8000.00 0.00 0.01799 17600.00 8000.00 0.00 0.02000 17800.00 8000.00 0.00 0.02202 18000.00 8000.00 0.00 0.02407 18200.00 8000.00 0.00 0.02614 18400.00 8000.00 0.00 0.02819 18600.00 8000.00 0.00 0.03015 18800.00 8000.00 0.00 0.03205 19000.00 8000.00 0.00 0.03398 19200.00 8000.00 0.00 0.03606 19400.00 8000.00 0.00 0.03840 19600.00 8000.00 0.00 0.04099 19800.00 8000.00 0.00 0.04378 20000.00 8000.00 0.00 0.04671 20200.00 8000.00 0.00 0.04978 20400.00 8000.00 0.00 0.05302 20600.00 8000.00 0.00 0.05645 20800.00 8000.00 0.00 0.06002 21000.00 8000.00 0.00 0.06349 21200.00 8000.00 0.00 0.06659 21400.00 8000.00 0.00 0.06910 21600.00 8000.00 0.00 0.07086 21800.00 8000.00 0.00 0.07191 22000.00 8000.00 0.00 0.07244 22200.00 8000.00 0.00 0.07265 22400.00 8000.00 0.00 0.07268 21800.00 8200.00 0.00 0.07272 22000.00 8200.00 0.00 0.07323 22200.00 8200.00 0.00 0.07343 22400.00 8200.00 0.00 0.07344 21800.00 8400.00 0.00 0.07346 22000.00 8400.00 0.00 0.07396 22200.00 8400.00 0.00 0.07414 22400.00 8400.00 0.00 0.07415 21800.00 8600.00 0.00 0.07428 22000.00 8600.00 0.00 0.07477 22200.00 8600.00 0.00 0.07494 22400.00 8600.00 0.00 0.07494 21800.00 8800.00 0.00 0.07520 22000.00 8800.00 0.00 0.07567 22200.00 8800.00 0.00 0.07584 22400.00 8800.00 0.00 0.07585 21800.00 9000.00 0.00 0.07615 22000.00 9000.00 0.00 0.07663 22200.00 9000.00 0.00 0.07681 22400.00 9000.00 0.00 0.07683 21800.00 9200.00 0.00 0.07713 22000.00 9200.00 0.00 0.07761 22200.00 9200.00 0.00 0.07780 22400.00 9200.00 0.00 0.07784 21800.00 9400.00 0.00 0.07809 22000.00 9400.00 0.00 0.07858 22200.00 9400.00 0.00 0.07879 22400.00 9400.00 0.00 0.07884 21800.00 9600.00 0.00 0.07903 22000.00 9600.00 0.00 0.07954 22200.00 9600.00 0.00 0.07975 22400.00 9600.00 0.00 0.07981 21800.00 9800.00 0.00 0.08007 22000.00 9800.00 0.00 0.08058 22200.00 9800.00 0.00 0.08079 22400.00 9800.00 0.00 0.08084 21800.00 10000.00 0.00 0.08121 22000.00 10000.00 0.00 0.08171 22200.00 10000.00 0.00 0.08192 22400.00 10000.00 0.00 0.08197 21800.00 10200.00 0.00 0.08235 22000.00 10200.00 0.00 0.08285 22200.00 10200.00 0.00 0.08305 22400.00 10200.00 0.00 0.08308 21800.00 10400.00 0.00 0.08342 22000.00 10400.00 0.00 0.08392 22200.00 10400.00 0.00 0.08411 22400.00 10400.00 0.00 0.08413 21800.00 10600.00 0.00 0.08442 22000.00 10600.00 0.00 0.08491 22200.00 10600.00 0.00 0.08510 22400.00 10600.00 0.00 0.08511 21800.00 10800.00 0.00 0.08534 22000.00 10800.00 0.00 0.08583 22200.00 10800.00 0.00 0.08601 21800.00 11000.00 0.00 0.08632 22000.00 11000.00 0.00 0.08681 22200.00 11000.00 0.00 0.08697 21800.00 11200.00 0.00 0.08750 22000.00 11200.00 0.00 0.08798 22200.00 11200.00 0.00 0.08813 21800.00 11400.00 0.00 0.08885 22000.00 11400.00 0.00 0.08931 22200.00 11400.00 0.00 0.08943 21800.00 11600.00 0.00 0.09027 22000.00 11600.00 0.00 0.09070 22200.00 11600.00 0.00 0.09079 21800.00 11800.00 0.00 0.09171 22000.00 11800.00 0.00 0.09211 22200.00 11800.00 0.00 0.09216 21600.00 12000.00 0.00 0.09218 21800.00 12000.00 0.00 0.09311 22000.00 12000.00 0.00 0.09347 22200.00 12000.00 0.00 0.09348 21600.00 12200.00 0.00 0.09361 21800.00 12200.00 0.00 0.09450 22000.00 12200.00 0.00 0.09484 21600.00 12400.00 0.00 0.09516 21800.00 12400.00 0.00 0.09603 22000.00 12400.00 0.00 0.09634 21600.00 12600.00 0.00 0.09686 21800.00 12600.00 0.00 0.09771 22000.00 12600.00 0.00 0.09800 21600.00 12800.00 0.00 0.09862 21800.00 12800.00 0.00 0.09944 22000.00 12800.00 0.00 0.09972 21600.00 13000.00 0.00 0.10039 21800.00 13000.00 0.00 0.10119 22000.00 13000.00 0.00 0.10144 21600.00 13200.00 0.00 0.10210 21800.00 13200.00 0.00 0.10288 22000.00 13200.00 0.00 0.10312 21600.00 13400.00 0.00 0.10378 21800.00 13400.00 0.00 0.10455 22000.00 13400.00 0.00 0.10478 21600.00 13600.00 0.00 0.10560 21800.00 13600.00 0.00 0.10636 22000.00 13600.00 0.00 0.10660 21600.00 13800.00 0.00 0.10763 21800.00 13800.00 0.00 0.10840 22000.00 13800.00 0.00 0.10865 21600.00 14000.00 0.00 0.10980 21800.00 14000.00 0.00 0.11058 22000.00 14000.00 0.00 0.11084 21600.00 14200.00 0.00 0.11203 21800.00 14200.00 0.00 0.11282 22000.00 14200.00 0.00 0.11307 21600.00 14400.00 0.00 0.11423 21800.00 14400.00 0.00 0.11501 22000.00 14400.00 0.00 0.11525 21600.00 14600.00 0.00 0.11631 21800.00 14600.00 0.00 0.11708 22000.00 14600.00 0.00 0.11730 21600.00 14800.00 0.00 0.11840 21800.00 14800.00 0.00 0.11915 22000.00 14800.00 0.00 0.11935 21600.00 15000.00 0.00 0.12068 21800.00 15000.00 0.00 0.12140 22000.00 15000.00 0.00 0.12155 21400.00 15200.00 0.00 0.12157 21600.00 15200.00 0.00 0.12311 21800.00 15200.00 0.00 0.12380 22000.00 15200.00 0.00 0.12390 21400.00 15400.00 0.00 0.12409 21600.00 15400.00 0.00 0.12560 21800.00 15400.00 0.00 0.12625 22000.00 15400.00 0.00 0.12630 21400.00 15600.00 0.00 0.12661 21600.00 15600.00 0.00 0.12810 21800.00 15600.00 0.00 0.12872 22000.00 15600.00 0.00 0.12873 21400.00 15800.00 0.00 0.12906 21600.00 15800.00 0.00 0.13054 21800.00 15800.00 0.00 0.13113 21400.00 16000.00 0.00 0.13149 21600.00 16000.00 0.00 0.13295 21800.00 16000.00 0.00 0.13353 21400.00 16200.00 0.00 0.13413 21600.00 16200.00 0.00 0.13558 21800.00 16200.00 0.00 0.13612 21400.00 16400.00 0.00 0.13703 21600.00 16400.00 0.00 0.13846 21800.00 16400.00 0.00 0.13896 21400.00 16600.00 0.00 0.14008 21600.00 16600.00 0.00 0.14148 21800.00 16600.00 0.00 0.14194 21400.00 16800.00 0.00 0.14323 21600.00 16800.00 0.00 0.14461 21800.00 16800.00 0.00 0.14502 21400.00 17000.00 0.00 0.14638 21600.00 17000.00 0.00 0.14772 21800.00 17000.00 0.00 0.14809 21400.00 17200.00 0.00 0.14942 21600.00 17200.00 0.00 0.15073 21800.00 17200.00 0.00 0.15105 21400.00 17400.00 0.00 0.15251 21600.00 17400.00 0.00 0.15379 21800.00 17400.00 0.00 0.15406 21400.00 17600.00 0.00 0.15581 21600.00 17600.00 0.00 0.15704 21800.00 17600.00 0.00 0.15724 21400.00 17800.00 0.00 0.15918 21600.00 17800.00 0.00 0.16036 21800.00 17800.00 0.00 0.16048 21400.00 18000.00 0.00 0.16251 21600.00 18000.00 0.00 0.16364 21800.00 18000.00 0.00 0.16368 21400.00 18200.00 0.00 0.16579 21600.00 18200.00 0.00 0.16685 21400.00 18400.00 0.00 0.16893 21600.00 18400.00 0.00 0.16992 21400.00 18600.00 0.00 0.17209 21600.00 18600.00 0.00 0.17300 21200.00 18800.00 0.00 0.17340 21400.00 18800.00 0.00 0.17556 21600.00 18800.00 0.00 0.17636 21200.00 19000.00 0.00 0.17725 21400.00 19000.00 0.00 0.17930 21600.00 19000.00 0.00 0.17997 21200.00 19200.00 0.00 0.18120 21400.00 19200.00 0.00 0.18314 21600.00 19200.00 0.00 0.18364 21200.00 19400.00 0.00 0.18515 21400.00 19400.00 0.00 0.18695 21600.00 19400.00 0.00 0.18728 21200.00 19600.00 0.00 0.18892 21400.00 19600.00 0.00 0.19059 21600.00 19600.00 0.00 0.19075 21200.00 19800.00 0.00 0.19259 21400.00 19800.00 0.00 0.19413 21200.00 20000.00 0.00 0.19662 21400.00 20000.00 0.00 0.19799 21000.00 20200.00 0.00 0.19829 21200.00 20200.00 0.00 0.20122 21400.00 20200.00 0.00 0.20234 21000.00 20400.00 0.00 0.20345 21200.00 20400.00 0.00 0.20611 21400.00 20400.00 0.00 0.20687 21000.00 20600.00 0.00 0.20868 21200.00 20600.00 0.00 0.21092 21400.00 20600.00 0.00 0.21122 21000.00 20800.00 0.00 0.21332 21200.00 20800.00 0.00 0.21498 21000.00 21000.00 0.00 0.21638 21200.00 21000.00 0.00 0.21740 21000.00 21200.00 0.00 0.21757 21200.00 21200.00 0.00 0.21800 21000.00 21200.00 30.00 0.21803 21000.00 21200.00 35.00 0.21805 21000.00 21200.00 40.00 0.21806 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21067.29 21272.11 36.00 0.21834 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/107.ctf NX, NY, NZ: 360 360 1 MODE : real