CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/104.mrc Output diagnostic file name Micrographs/104.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/104.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2210.000 8760.000 Mean, RMS : 4231.816 215.4026 TITLE 1: emi2mrc: 104.emi Sun Jan 19 18:23:05 2014 TITLE 2: kV 300, Mag 78000x, Def 22150, Spot 4, Int 55.13%, Obj 88.07%, Dif 73.67% TITLE 3: X 87.753, Y 693.080, Z -34.314, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00733 8200.00 8000.00 0.00 -0.00707 8400.00 8000.00 0.00 -0.00672 8600.00 8000.00 0.00 -0.00637 8800.00 8000.00 0.00 -0.00608 9000.00 8000.00 0.00 -0.00584 9200.00 8000.00 0.00 -0.00562 9400.00 8000.00 0.00 -0.00538 9600.00 8000.00 0.00 -0.00507 9800.00 8000.00 0.00 -0.00464 10000.00 8000.00 0.00 -0.00409 10200.00 8000.00 0.00 -0.00339 10400.00 8000.00 0.00 -0.00255 10600.00 8000.00 0.00 -0.00161 10800.00 8000.00 0.00 -0.00062 11000.00 8000.00 0.00 0.00036 11200.00 8000.00 0.00 0.00128 11400.00 8000.00 0.00 0.00216 11600.00 8000.00 0.00 0.00301 11800.00 8000.00 0.00 0.00387 12000.00 8000.00 0.00 0.00477 12200.00 8000.00 0.00 0.00573 12400.00 8000.00 0.00 0.00678 12600.00 8000.00 0.00 0.00796 12800.00 8000.00 0.00 0.00928 13000.00 8000.00 0.00 0.01075 13200.00 8000.00 0.00 0.01232 13400.00 8000.00 0.00 0.01394 13600.00 8000.00 0.00 0.01556 13800.00 8000.00 0.00 0.01718 14000.00 8000.00 0.00 0.01883 14200.00 8000.00 0.00 0.02057 14400.00 8000.00 0.00 0.02242 14600.00 8000.00 0.00 0.02440 14800.00 8000.00 0.00 0.02650 15000.00 8000.00 0.00 0.02873 15200.00 8000.00 0.00 0.03113 15400.00 8000.00 0.00 0.03371 15600.00 8000.00 0.00 0.03649 15800.00 8000.00 0.00 0.03942 16000.00 8000.00 0.00 0.04237 16200.00 8000.00 0.00 0.04525 16400.00 8000.00 0.00 0.04802 16600.00 8000.00 0.00 0.05066 16800.00 8000.00 0.00 0.05324 17000.00 8000.00 0.00 0.05584 17200.00 8000.00 0.00 0.05850 17400.00 8000.00 0.00 0.06122 17600.00 8000.00 0.00 0.06399 17800.00 8000.00 0.00 0.06676 18000.00 8000.00 0.00 0.06950 18200.00 8000.00 0.00 0.07227 18400.00 8000.00 0.00 0.07512 18600.00 8000.00 0.00 0.07810 18800.00 8000.00 0.00 0.08118 19000.00 8000.00 0.00 0.08421 19200.00 8000.00 0.00 0.08692 19400.00 8000.00 0.00 0.08907 19600.00 8000.00 0.00 0.09056 19800.00 8000.00 0.00 0.09143 20000.00 8000.00 0.00 0.09190 20200.00 8000.00 0.00 0.09221 20400.00 8000.00 0.00 0.09246 20600.00 8000.00 0.00 0.09269 20800.00 8000.00 0.00 0.09283 20000.00 8200.00 0.00 0.09290 20200.00 8200.00 0.00 0.09322 20400.00 8200.00 0.00 0.09348 20600.00 8200.00 0.00 0.09372 20800.00 8200.00 0.00 0.09386 20000.00 8400.00 0.00 0.09399 20200.00 8400.00 0.00 0.09433 20400.00 8400.00 0.00 0.09462 20600.00 8400.00 0.00 0.09487 20800.00 8400.00 0.00 0.09501 20000.00 8600.00 0.00 0.09537 20200.00 8600.00 0.00 0.09573 20400.00 8600.00 0.00 0.09604 20600.00 8600.00 0.00 0.09629 20800.00 8600.00 0.00 0.09642 19800.00 8800.00 0.00 0.09649 20000.00 8800.00 0.00 0.09703 20200.00 8800.00 0.00 0.09740 20400.00 8800.00 0.00 0.09771 20600.00 8800.00 0.00 0.09795 20800.00 8800.00 0.00 0.09805 19800.00 9000.00 0.00 0.09825 20000.00 9000.00 0.00 0.09879 20200.00 9000.00 0.00 0.09915 20400.00 9000.00 0.00 0.09944 20600.00 9000.00 0.00 0.09964 20800.00 9000.00 0.00 0.09970 19800.00 9200.00 0.00 0.09998 20000.00 9200.00 0.00 0.10050 20200.00 9200.00 0.00 0.10084 20400.00 9200.00 0.00 0.10109 20600.00 9200.00 0.00 0.10124 19800.00 9400.00 0.00 0.10152 20000.00 9400.00 0.00 0.10202 20200.00 9400.00 0.00 0.10232 20400.00 9400.00 0.00 0.10251 20600.00 9400.00 0.00 0.10261 19800.00 9600.00 0.00 0.10283 20000.00 9600.00 0.00 0.10329 20200.00 9600.00 0.00 0.10353 20400.00 9600.00 0.00 0.10367 20600.00 9600.00 0.00 0.10370 19800.00 9800.00 0.00 0.10405 20000.00 9800.00 0.00 0.10447 20200.00 9800.00 0.00 0.10466 20400.00 9800.00 0.00 0.10474 19800.00 10000.00 0.00 0.10534 20000.00 10000.00 0.00 0.10572 20200.00 10000.00 0.00 0.10586 20400.00 10000.00 0.00 0.10589 19800.00 10200.00 0.00 0.10671 20000.00 10200.00 0.00 0.10705 20200.00 10200.00 0.00 0.10716 19600.00 10400.00 0.00 0.10739 19800.00 10400.00 0.00 0.10821 20000.00 10400.00 0.00 0.10853 20200.00 10400.00 0.00 0.10862 19600.00 10600.00 0.00 0.10903 19800.00 10600.00 0.00 0.10984 20000.00 10600.00 0.00 0.11016 20200.00 10600.00 0.00 0.11025 19600.00 10800.00 0.00 0.11075 19800.00 10800.00 0.00 0.11157 20000.00 10800.00 0.00 0.11189 20200.00 10800.00 0.00 0.11197 19600.00 11000.00 0.00 0.11262 19800.00 11000.00 0.00 0.11344 20000.00 11000.00 0.00 0.11373 20200.00 11000.00 0.00 0.11379 19600.00 11200.00 0.00 0.11465 19800.00 11200.00 0.00 0.11544 20000.00 11200.00 0.00 0.11570 20200.00 11200.00 0.00 0.11572 19600.00 11400.00 0.00 0.11670 19800.00 11400.00 0.00 0.11746 20000.00 11400.00 0.00 0.11767 19600.00 11600.00 0.00 0.11868 19800.00 11600.00 0.00 0.11939 20000.00 11600.00 0.00 0.11954 19600.00 11800.00 0.00 0.12058 19800.00 11800.00 0.00 0.12124 20000.00 11800.00 0.00 0.12134 19600.00 12000.00 0.00 0.12242 19800.00 12000.00 0.00 0.12304 20000.00 12000.00 0.00 0.12309 19600.00 12200.00 0.00 0.12434 19800.00 12200.00 0.00 0.12494 20000.00 12200.00 0.00 0.12496 19400.00 12400.00 0.00 0.12514 19600.00 12400.00 0.00 0.12658 19800.00 12400.00 0.00 0.12715 19400.00 12600.00 0.00 0.12771 19600.00 12600.00 0.00 0.12914 19800.00 12600.00 0.00 0.12968 19400.00 12800.00 0.00 0.13047 19600.00 12800.00 0.00 0.13187 19800.00 12800.00 0.00 0.13238 19400.00 13000.00 0.00 0.13332 19600.00 13000.00 0.00 0.13469 19800.00 13000.00 0.00 0.13516 19400.00 13200.00 0.00 0.13616 19600.00 13200.00 0.00 0.13749 19800.00 13200.00 0.00 0.13790 19400.00 13400.00 0.00 0.13889 19600.00 13400.00 0.00 0.14016 19800.00 13400.00 0.00 0.14049 19400.00 13600.00 0.00 0.14162 19600.00 13600.00 0.00 0.14282 19800.00 13600.00 0.00 0.14308 19400.00 13800.00 0.00 0.14440 19600.00 13800.00 0.00 0.14554 19800.00 13800.00 0.00 0.14573 19400.00 14000.00 0.00 0.14713 19600.00 14000.00 0.00 0.14821 19800.00 14000.00 0.00 0.14836 19400.00 14200.00 0.00 0.14979 19600.00 14200.00 0.00 0.15085 19800.00 14200.00 0.00 0.15096 19400.00 14400.00 0.00 0.15244 19600.00 14400.00 0.00 0.15346 19800.00 14400.00 0.00 0.15354 19400.00 14600.00 0.00 0.15502 19600.00 14600.00 0.00 0.15601 19800.00 14600.00 0.00 0.15604 19400.00 14800.00 0.00 0.15769 19600.00 14800.00 0.00 0.15864 19200.00 15000.00 0.00 0.15867 19400.00 15000.00 0.00 0.16062 19600.00 15000.00 0.00 0.16152 19200.00 15200.00 0.00 0.16178 19400.00 15200.00 0.00 0.16369 19600.00 15200.00 0.00 0.16453 19200.00 15400.00 0.00 0.16486 19400.00 15400.00 0.00 0.16673 19600.00 15400.00 0.00 0.16751 19200.00 15600.00 0.00 0.16791 19400.00 15600.00 0.00 0.16974 19600.00 15600.00 0.00 0.17047 19200.00 15800.00 0.00 0.17090 19400.00 15800.00 0.00 0.17269 19600.00 15800.00 0.00 0.17336 19200.00 16000.00 0.00 0.17388 19400.00 16000.00 0.00 0.17563 19600.00 16000.00 0.00 0.17625 19200.00 16200.00 0.00 0.17712 19400.00 16200.00 0.00 0.17883 19600.00 16200.00 0.00 0.17939 19200.00 16400.00 0.00 0.18061 19400.00 16400.00 0.00 0.18228 19600.00 16400.00 0.00 0.18279 19200.00 16600.00 0.00 0.18414 19400.00 16600.00 0.00 0.18576 19600.00 16600.00 0.00 0.18620 19200.00 16800.00 0.00 0.18759 19400.00 16800.00 0.00 0.18916 19600.00 16800.00 0.00 0.18954 19200.00 17000.00 0.00 0.19091 19400.00 17000.00 0.00 0.19243 19600.00 17000.00 0.00 0.19273 19200.00 17200.00 0.00 0.19405 19400.00 17200.00 0.00 0.19551 19600.00 17200.00 0.00 0.19571 19200.00 17400.00 0.00 0.19728 19400.00 17400.00 0.00 0.19865 19600.00 17400.00 0.00 0.19873 19200.00 17600.00 0.00 0.20075 19400.00 17600.00 0.00 0.20201 19200.00 17800.00 0.00 0.20426 19400.00 17800.00 0.00 0.20538 19000.00 18000.00 0.00 0.20539 19200.00 18000.00 0.00 0.20767 19400.00 18000.00 0.00 0.20863 19000.00 18200.00 0.00 0.20881 19200.00 18200.00 0.00 0.21096 19400.00 18200.00 0.00 0.21172 19000.00 18400.00 0.00 0.21193 19200.00 18400.00 0.00 0.21393 19400.00 18400.00 0.00 0.21446 19000.00 18600.00 0.00 0.21486 19200.00 18600.00 0.00 0.21669 19400.00 18600.00 0.00 0.21697 19000.00 18800.00 0.00 0.21790 19200.00 18800.00 0.00 0.21947 19000.00 19000.00 0.00 0.22062 19200.00 19000.00 0.00 0.22180 19000.00 19200.00 0.00 0.22222 19200.00 19200.00 0.00 0.22290 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19145.21 19285.52 21.94 0.22305 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/104.ctf NX, NY, NZ: 360 360 1 MODE : real