CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/066.mrc Output diagnostic file name Micrographs/066.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/066.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2150.000 8370.000 Mean, RMS : 4401.492 228.9266 TITLE 1: emi2mrc: 066.emi Sun Jan 19 14:59:07 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 272.722, Y -465.544, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 112 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03053 13800.00 8000.00 0.00 -0.03036 14000.00 8000.00 0.00 -0.02974 14200.00 8000.00 0.00 -0.02914 14400.00 8000.00 0.00 -0.02849 14600.00 8000.00 0.00 -0.02772 14800.00 8000.00 0.00 -0.02680 15000.00 8000.00 0.00 -0.02580 15200.00 8000.00 0.00 -0.02482 15400.00 8000.00 0.00 -0.02394 15600.00 8000.00 0.00 -0.02318 15800.00 8000.00 0.00 -0.02249 16000.00 8000.00 0.00 -0.02180 16200.00 8000.00 0.00 -0.02108 16400.00 8000.00 0.00 -0.02032 16600.00 8000.00 0.00 -0.01954 16800.00 8000.00 0.00 -0.01873 17000.00 8000.00 0.00 -0.01786 17200.00 8000.00 0.00 -0.01688 17400.00 8000.00 0.00 -0.01573 17600.00 8000.00 0.00 -0.01443 17800.00 8000.00 0.00 -0.01301 18000.00 8000.00 0.00 -0.01152 18200.00 8000.00 0.00 -0.01001 18400.00 8000.00 0.00 -0.00847 18600.00 8000.00 0.00 -0.00691 18800.00 8000.00 0.00 -0.00529 19000.00 8000.00 0.00 -0.00361 19200.00 8000.00 0.00 -0.00187 19400.00 8000.00 0.00 -0.00011 19600.00 8000.00 0.00 0.00163 19800.00 8000.00 0.00 0.00332 20000.00 8000.00 0.00 0.00496 20200.00 8000.00 0.00 0.00656 20400.00 8000.00 0.00 0.00814 20600.00 8000.00 0.00 0.00970 20800.00 8000.00 0.00 0.01127 21000.00 8000.00 0.00 0.01286 21200.00 8000.00 0.00 0.01453 21400.00 8000.00 0.00 0.01632 21600.00 8000.00 0.00 0.01826 21800.00 8000.00 0.00 0.02034 22000.00 8000.00 0.00 0.02252 22200.00 8000.00 0.00 0.02476 22400.00 8000.00 0.00 0.02703 22600.00 8000.00 0.00 0.02939 22800.00 8000.00 0.00 0.03195 23000.00 8000.00 0.00 0.03479 23200.00 8000.00 0.00 0.03794 23400.00 8000.00 0.00 0.04125 23600.00 8000.00 0.00 0.04448 23800.00 8000.00 0.00 0.04736 24000.00 8000.00 0.00 0.04965 24200.00 8000.00 0.00 0.05122 24400.00 8000.00 0.00 0.05211 24600.00 8000.00 0.00 0.05248 24800.00 8000.00 0.00 0.05251 24600.00 8200.00 0.00 0.05265 24800.00 8200.00 0.00 0.05267 24600.00 8400.00 0.00 0.05289 24800.00 8400.00 0.00 0.05290 24400.00 8600.00 0.00 0.05295 24600.00 8600.00 0.00 0.05330 24800.00 8600.00 0.00 0.05333 24400.00 8800.00 0.00 0.05355 24600.00 8800.00 0.00 0.05392 24800.00 8800.00 0.00 0.05397 24400.00 9000.00 0.00 0.05426 24600.00 9000.00 0.00 0.05465 24800.00 9000.00 0.00 0.05472 24400.00 9200.00 0.00 0.05496 24600.00 9200.00 0.00 0.05537 24800.00 9200.00 0.00 0.05547 24400.00 9400.00 0.00 0.05555 24600.00 9400.00 0.00 0.05598 24800.00 9400.00 0.00 0.05610 24600.00 9600.00 0.00 0.05644 24800.00 9600.00 0.00 0.05658 25000.00 9600.00 0.00 0.05658 24600.00 9800.00 0.00 0.05684 24800.00 9800.00 0.00 0.05700 25000.00 9800.00 0.00 0.05701 24600.00 10000.00 0.00 0.05730 24800.00 10000.00 0.00 0.05747 25000.00 10000.00 0.00 0.05750 24600.00 10200.00 0.00 0.05787 24800.00 10200.00 0.00 0.05806 25000.00 10200.00 0.00 0.05808 24600.00 10400.00 0.00 0.05857 24800.00 10400.00 0.00 0.05877 25000.00 10400.00 0.00 0.05880 24400.00 10600.00 0.00 0.05881 24600.00 10600.00 0.00 0.05938 24800.00 10600.00 0.00 0.05960 25000.00 10600.00 0.00 0.05964 24400.00 10800.00 0.00 0.05964 24600.00 10800.00 0.00 0.06024 24800.00 10800.00 0.00 0.06048 25000.00 10800.00 0.00 0.06051 24600.00 11000.00 0.00 0.06113 24800.00 11000.00 0.00 0.06137 25000.00 11000.00 0.00 0.06139 24400.00 11200.00 0.00 0.06141 24600.00 11200.00 0.00 0.06205 24800.00 11200.00 0.00 0.06229 25000.00 11200.00 0.00 0.06229 24400.00 11400.00 0.00 0.06229 24600.00 11400.00 0.00 0.06295 24800.00 11400.00 0.00 0.06317 24600.00 11600.00 0.00 0.06379 24800.00 11600.00 0.00 0.06401 24600.00 11800.00 0.00 0.06461 24800.00 11800.00 0.00 0.06481 24600.00 12000.00 0.00 0.06540 24800.00 12000.00 0.00 0.06558 24400.00 12200.00 0.00 0.06560 24600.00 12200.00 0.00 0.06623 24800.00 12200.00 0.00 0.06638 24400.00 12400.00 0.00 0.06660 24600.00 12400.00 0.00 0.06720 24800.00 12400.00 0.00 0.06732 24400.00 12600.00 0.00 0.06776 24600.00 12600.00 0.00 0.06834 24800.00 12600.00 0.00 0.06842 24400.00 12800.00 0.00 0.06905 24600.00 12800.00 0.00 0.06960 24800.00 12800.00 0.00 0.06964 24400.00 13000.00 0.00 0.07044 24600.00 13000.00 0.00 0.07095 24400.00 13200.00 0.00 0.07186 24600.00 13200.00 0.00 0.07234 24400.00 13400.00 0.00 0.07326 24600.00 13400.00 0.00 0.07369 24400.00 13600.00 0.00 0.07467 24600.00 13600.00 0.00 0.07504 24400.00 13800.00 0.00 0.07610 24600.00 13800.00 0.00 0.07642 24200.00 14000.00 0.00 0.07647 24400.00 14000.00 0.00 0.07750 24600.00 14000.00 0.00 0.07777 24200.00 14200.00 0.00 0.07787 24400.00 14200.00 0.00 0.07887 24600.00 14200.00 0.00 0.07909 24200.00 14400.00 0.00 0.07925 24400.00 14400.00 0.00 0.08021 24600.00 14400.00 0.00 0.08040 24200.00 14600.00 0.00 0.08063 24400.00 14600.00 0.00 0.08156 24600.00 14600.00 0.00 0.08173 24200.00 14800.00 0.00 0.08210 24400.00 14800.00 0.00 0.08302 24600.00 14800.00 0.00 0.08317 24200.00 15000.00 0.00 0.08378 24400.00 15000.00 0.00 0.08468 24600.00 15000.00 0.00 0.08482 24200.00 15200.00 0.00 0.08563 24400.00 15200.00 0.00 0.08651 24600.00 15200.00 0.00 0.08664 24200.00 15400.00 0.00 0.08757 24400.00 15400.00 0.00 0.08841 24600.00 15400.00 0.00 0.08852 24200.00 15600.00 0.00 0.08951 24400.00 15600.00 0.00 0.09032 24600.00 15600.00 0.00 0.09038 24200.00 15800.00 0.00 0.09137 24400.00 15800.00 0.00 0.09214 24600.00 15800.00 0.00 0.09217 24200.00 16000.00 0.00 0.09319 24400.00 16000.00 0.00 0.09393 24600.00 16000.00 0.00 0.09393 24200.00 16200.00 0.00 0.09512 24400.00 16200.00 0.00 0.09582 24200.00 16400.00 0.00 0.09718 24400.00 16400.00 0.00 0.09786 24200.00 16600.00 0.00 0.09932 24400.00 16600.00 0.00 0.09998 24200.00 16800.00 0.00 0.10149 24400.00 16800.00 0.00 0.10213 24200.00 17000.00 0.00 0.10362 24400.00 17000.00 0.00 0.10424 24200.00 17200.00 0.00 0.10568 24400.00 17200.00 0.00 0.10629 24200.00 17400.00 0.00 0.10783 24400.00 17400.00 0.00 0.10845 24000.00 17600.00 0.00 0.10860 24200.00 17600.00 0.00 0.11020 24400.00 17600.00 0.00 0.11082 24000.00 17800.00 0.00 0.11113 24200.00 17800.00 0.00 0.11274 24400.00 17800.00 0.00 0.11334 24000.00 18000.00 0.00 0.11375 24200.00 18000.00 0.00 0.11535 24400.00 18000.00 0.00 0.11594 24000.00 18200.00 0.00 0.11633 24200.00 18200.00 0.00 0.11793 24400.00 18200.00 0.00 0.11851 24000.00 18400.00 0.00 0.11876 24200.00 18400.00 0.00 0.12036 24400.00 18400.00 0.00 0.12094 24000.00 18600.00 0.00 0.12112 24200.00 18600.00 0.00 0.12274 24400.00 18600.00 0.00 0.12331 24000.00 18800.00 0.00 0.12365 24200.00 18800.00 0.00 0.12529 24400.00 18800.00 0.00 0.12585 24000.00 19000.00 0.00 0.12642 24200.00 19000.00 0.00 0.12807 24400.00 19000.00 0.00 0.12862 24000.00 19200.00 0.00 0.12939 24200.00 19200.00 0.00 0.13104 24400.00 19200.00 0.00 0.13158 24000.00 19400.00 0.00 0.13250 24200.00 19400.00 0.00 0.13415 24400.00 19400.00 0.00 0.13466 24000.00 19600.00 0.00 0.13553 24200.00 19600.00 0.00 0.13717 24400.00 19600.00 0.00 0.13764 24000.00 19800.00 0.00 0.13831 24200.00 19800.00 0.00 0.13993 24400.00 19800.00 0.00 0.14034 24000.00 20000.00 0.00 0.14096 24200.00 20000.00 0.00 0.14255 24400.00 20000.00 0.00 0.14291 24000.00 20200.00 0.00 0.14359 24200.00 20200.00 0.00 0.14514 24400.00 20200.00 0.00 0.14544 24000.00 20400.00 0.00 0.14621 24200.00 20400.00 0.00 0.14774 24400.00 20400.00 0.00 0.14797 24000.00 20600.00 0.00 0.14896 24200.00 20600.00 0.00 0.15046 24400.00 20600.00 0.00 0.15063 24000.00 20800.00 0.00 0.15190 24200.00 20800.00 0.00 0.15338 24400.00 20800.00 0.00 0.15349 24000.00 21000.00 0.00 0.15498 24200.00 21000.00 0.00 0.15643 24400.00 21000.00 0.00 0.15644 24000.00 21200.00 0.00 0.15833 24200.00 21200.00 0.00 0.15970 24000.00 21400.00 0.00 0.16203 24200.00 21400.00 0.00 0.16329 24000.00 21600.00 0.00 0.16593 24200.00 21600.00 0.00 0.16704 23800.00 21800.00 0.00 0.16720 24000.00 21800.00 0.00 0.16986 24200.00 21800.00 0.00 0.17080 23800.00 22000.00 0.00 0.17119 24000.00 22000.00 0.00 0.17373 24200.00 22000.00 0.00 0.17449 23800.00 22200.00 0.00 0.17498 24000.00 22200.00 0.00 0.17740 24200.00 22200.00 0.00 0.17798 23800.00 22400.00 0.00 0.17872 24000.00 22400.00 0.00 0.18101 24200.00 22400.00 0.00 0.18138 23800.00 22600.00 0.00 0.18284 24000.00 22600.00 0.00 0.18494 24200.00 22600.00 0.00 0.18506 23800.00 22800.00 0.00 0.18746 24000.00 22800.00 0.00 0.18931 23800.00 23000.00 0.00 0.19248 24000.00 23000.00 0.00 0.19397 23600.00 23200.00 0.00 0.19459 23800.00 23200.00 0.00 0.19764 24000.00 23200.00 0.00 0.19865 23600.00 23400.00 0.00 0.19970 23800.00 23400.00 0.00 0.20217 24000.00 23400.00 0.00 0.20259 23600.00 23600.00 0.00 0.20332 23800.00 23600.00 0.00 0.20515 23800.00 23800.00 0.00 0.20630 23600.00 24000.00 30.00 0.20639 23600.00 24000.00 35.00 0.20652 23600.00 24000.00 40.00 0.20659 23600.00 24000.00 45.00 0.20662 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23696.96 23957.14 44.43 0.20687 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/066.ctf NX, NY, NZ: 360 360 1 MODE : real