CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/060.mrc Output diagnostic file name Micrographs/060.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/060.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1652.000 8272.000 Mean, RMS : 4398.210 223.9102 TITLE 1: emi2mrc: 060.emi Sun Jan 19 14:54:12 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 265.659, Y -474.901, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03960 13400.00 8000.00 0.00 -0.03946 13600.00 8000.00 0.00 -0.03913 13800.00 8000.00 0.00 -0.03884 14000.00 8000.00 0.00 -0.03857 14200.00 8000.00 0.00 -0.03825 14400.00 8000.00 0.00 -0.03779 14600.00 8000.00 0.00 -0.03712 14800.00 8000.00 0.00 -0.03623 15000.00 8000.00 0.00 -0.03521 15200.00 8000.00 0.00 -0.03415 15400.00 8000.00 0.00 -0.03315 15600.00 8000.00 0.00 -0.03224 15800.00 8000.00 0.00 -0.03141 16000.00 8000.00 0.00 -0.03059 16200.00 8000.00 0.00 -0.02975 16400.00 8000.00 0.00 -0.02887 16600.00 8000.00 0.00 -0.02793 16800.00 8000.00 0.00 -0.02694 17000.00 8000.00 0.00 -0.02589 17200.00 8000.00 0.00 -0.02477 17400.00 8000.00 0.00 -0.02358 17600.00 8000.00 0.00 -0.02232 17800.00 8000.00 0.00 -0.02096 18000.00 8000.00 0.00 -0.01950 18200.00 8000.00 0.00 -0.01796 18400.00 8000.00 0.00 -0.01636 18600.00 8000.00 0.00 -0.01472 18800.00 8000.00 0.00 -0.01308 19000.00 8000.00 0.00 -0.01148 19200.00 8000.00 0.00 -0.00991 19400.00 8000.00 0.00 -0.00835 19600.00 8000.00 0.00 -0.00678 19800.00 8000.00 0.00 -0.00516 20000.00 8000.00 0.00 -0.00346 20200.00 8000.00 0.00 -0.00167 20400.00 8000.00 0.00 0.00019 20600.00 8000.00 0.00 0.00209 20800.00 8000.00 0.00 0.00399 21000.00 8000.00 0.00 0.00585 21200.00 8000.00 0.00 0.00765 21400.00 8000.00 0.00 0.00940 21600.00 8000.00 0.00 0.01115 21800.00 8000.00 0.00 0.01291 22000.00 8000.00 0.00 0.01473 22200.00 8000.00 0.00 0.01662 22400.00 8000.00 0.00 0.01858 22600.00 8000.00 0.00 0.02061 22800.00 8000.00 0.00 0.02268 23000.00 8000.00 0.00 0.02479 23200.00 8000.00 0.00 0.02693 23400.00 8000.00 0.00 0.02909 23600.00 8000.00 0.00 0.03126 23800.00 8000.00 0.00 0.03341 24000.00 8000.00 0.00 0.03551 24200.00 8000.00 0.00 0.03743 24400.00 8000.00 0.00 0.03906 24600.00 8000.00 0.00 0.04030 24800.00 8000.00 0.00 0.04112 25000.00 8000.00 0.00 0.04154 25200.00 8000.00 0.00 0.04170 25400.00 8000.00 0.00 0.04170 25000.00 8200.00 0.00 0.04175 25200.00 8200.00 0.00 0.04194 25400.00 8200.00 0.00 0.04196 25200.00 8400.00 0.00 0.04210 25400.00 8400.00 0.00 0.04214 25200.00 8600.00 0.00 0.04226 25400.00 8600.00 0.00 0.04232 25200.00 8800.00 0.00 0.04247 25400.00 8800.00 0.00 0.04255 25200.00 9000.00 0.00 0.04279 25400.00 9000.00 0.00 0.04288 25000.00 9200.00 0.00 0.04294 25200.00 9200.00 0.00 0.04324 25400.00 9200.00 0.00 0.04334 25000.00 9400.00 0.00 0.04353 25200.00 9400.00 0.00 0.04383 25400.00 9400.00 0.00 0.04392 25000.00 9600.00 0.00 0.04422 25200.00 9600.00 0.00 0.04451 25400.00 9600.00 0.00 0.04460 25000.00 9800.00 0.00 0.04500 25200.00 9800.00 0.00 0.04529 25400.00 9800.00 0.00 0.04536 25000.00 10000.00 0.00 0.04584 25200.00 10000.00 0.00 0.04611 25400.00 10000.00 0.00 0.04617 25000.00 10200.00 0.00 0.04667 25200.00 10200.00 0.00 0.04693 25400.00 10200.00 0.00 0.04699 25000.00 10400.00 0.00 0.04748 25200.00 10400.00 0.00 0.04775 25400.00 10400.00 0.00 0.04781 25000.00 10600.00 0.00 0.04828 25200.00 10600.00 0.00 0.04857 25400.00 10600.00 0.00 0.04864 25000.00 10800.00 0.00 0.04911 25200.00 10800.00 0.00 0.04941 25400.00 10800.00 0.00 0.04949 25000.00 11000.00 0.00 0.04999 25200.00 11000.00 0.00 0.05029 25400.00 11000.00 0.00 0.05037 25000.00 11200.00 0.00 0.05093 25200.00 11200.00 0.00 0.05123 25400.00 11200.00 0.00 0.05128 25000.00 11400.00 0.00 0.05187 25200.00 11400.00 0.00 0.05215 25400.00 11400.00 0.00 0.05216 25000.00 11600.00 0.00 0.05277 25200.00 11600.00 0.00 0.05300 24800.00 11800.00 0.00 0.05302 25000.00 11800.00 0.00 0.05361 25200.00 11800.00 0.00 0.05380 24800.00 12000.00 0.00 0.05386 25000.00 12000.00 0.00 0.05442 25200.00 12000.00 0.00 0.05456 24800.00 12200.00 0.00 0.05473 25000.00 12200.00 0.00 0.05526 25200.00 12200.00 0.00 0.05536 24800.00 12400.00 0.00 0.05571 25000.00 12400.00 0.00 0.05621 25200.00 12400.00 0.00 0.05628 24800.00 12600.00 0.00 0.05679 25000.00 12600.00 0.00 0.05725 25200.00 12600.00 0.00 0.05730 24800.00 12800.00 0.00 0.05790 25000.00 12800.00 0.00 0.05834 25200.00 12800.00 0.00 0.05837 24800.00 13000.00 0.00 0.05904 25000.00 13000.00 0.00 0.05945 25200.00 13000.00 0.00 0.05946 24800.00 13200.00 0.00 0.06018 25000.00 13200.00 0.00 0.06056 24800.00 13400.00 0.00 0.06133 25000.00 13400.00 0.00 0.06169 24800.00 13600.00 0.00 0.06256 25000.00 13600.00 0.00 0.06290 24600.00 13800.00 0.00 0.06300 24800.00 13800.00 0.00 0.06389 25000.00 13800.00 0.00 0.06421 24600.00 14000.00 0.00 0.06435 24800.00 14000.00 0.00 0.06523 25000.00 14000.00 0.00 0.06555 24600.00 14200.00 0.00 0.06567 24800.00 14200.00 0.00 0.06654 25000.00 14200.00 0.00 0.06687 25200.00 14200.00 0.00 0.06687 24600.00 14400.00 0.00 0.06696 24800.00 14400.00 0.00 0.06785 25000.00 14400.00 0.00 0.06818 25200.00 14400.00 0.00 0.06819 24600.00 14600.00 0.00 0.06829 24800.00 14600.00 0.00 0.06918 25000.00 14600.00 0.00 0.06952 25200.00 14600.00 0.00 0.06955 24600.00 14800.00 0.00 0.06976 24800.00 14800.00 0.00 0.07066 25000.00 14800.00 0.00 0.07100 25200.00 14800.00 0.00 0.07103 24600.00 15000.00 0.00 0.07146 24800.00 15000.00 0.00 0.07236 25000.00 15000.00 0.00 0.07269 25200.00 15000.00 0.00 0.07270 24600.00 15200.00 0.00 0.07332 24800.00 15200.00 0.00 0.07421 25000.00 15200.00 0.00 0.07452 24600.00 15400.00 0.00 0.07523 24800.00 15400.00 0.00 0.07608 25000.00 15400.00 0.00 0.07635 24600.00 15600.00 0.00 0.07707 24800.00 15600.00 0.00 0.07789 25000.00 15600.00 0.00 0.07811 24600.00 15800.00 0.00 0.07881 24800.00 15800.00 0.00 0.07958 25000.00 15800.00 0.00 0.07977 24600.00 16000.00 0.00 0.08048 24800.00 16000.00 0.00 0.08122 25000.00 16000.00 0.00 0.08138 24600.00 16200.00 0.00 0.08222 24800.00 16200.00 0.00 0.08295 25000.00 16200.00 0.00 0.08309 24600.00 16400.00 0.00 0.08408 24800.00 16400.00 0.00 0.08479 25000.00 16400.00 0.00 0.08492 24600.00 16600.00 0.00 0.08601 24800.00 16600.00 0.00 0.08671 25000.00 16600.00 0.00 0.08684 24600.00 16800.00 0.00 0.08797 24800.00 16800.00 0.00 0.08868 25000.00 16800.00 0.00 0.08879 24600.00 17000.00 0.00 0.08995 24800.00 17000.00 0.00 0.09064 25000.00 17000.00 0.00 0.09075 24600.00 17200.00 0.00 0.09193 24800.00 17200.00 0.00 0.09260 25000.00 17200.00 0.00 0.09268 24600.00 17400.00 0.00 0.09402 24800.00 17400.00 0.00 0.09467 25000.00 17400.00 0.00 0.09471 24400.00 17600.00 0.00 0.09490 24600.00 17600.00 0.00 0.09628 24800.00 17600.00 0.00 0.09688 24400.00 17800.00 0.00 0.09726 24600.00 17800.00 0.00 0.09859 24800.00 17800.00 0.00 0.09914 24400.00 18000.00 0.00 0.09958 24600.00 18000.00 0.00 0.10086 24800.00 18000.00 0.00 0.10137 24400.00 18200.00 0.00 0.10179 24600.00 18200.00 0.00 0.10305 24800.00 18200.00 0.00 0.10352 24400.00 18400.00 0.00 0.10387 24600.00 18400.00 0.00 0.10510 24800.00 18400.00 0.00 0.10555 24400.00 18600.00 0.00 0.10594 24600.00 18600.00 0.00 0.10716 24800.00 18600.00 0.00 0.10757 24400.00 18800.00 0.00 0.10817 24600.00 18800.00 0.00 0.10938 24800.00 18800.00 0.00 0.10978 24400.00 19000.00 0.00 0.11057 24600.00 19000.00 0.00 0.11177 24800.00 19000.00 0.00 0.11216 24400.00 19200.00 0.00 0.11305 24600.00 19200.00 0.00 0.11424 24800.00 19200.00 0.00 0.11462 24400.00 19400.00 0.00 0.11557 24600.00 19400.00 0.00 0.11675 24800.00 19400.00 0.00 0.11711 24400.00 19600.00 0.00 0.11800 24600.00 19600.00 0.00 0.11915 24800.00 19600.00 0.00 0.11948 24400.00 19800.00 0.00 0.12031 24600.00 19800.00 0.00 0.12143 24800.00 19800.00 0.00 0.12173 24400.00 20000.00 0.00 0.12266 24600.00 20000.00 0.00 0.12376 24800.00 20000.00 0.00 0.12403 24400.00 20200.00 0.00 0.12514 24600.00 20200.00 0.00 0.12621 24800.00 20200.00 0.00 0.12645 24400.00 20400.00 0.00 0.12764 24600.00 20400.00 0.00 0.12869 24800.00 20400.00 0.00 0.12890 24400.00 20600.00 0.00 0.13015 24600.00 20600.00 0.00 0.13118 24800.00 20600.00 0.00 0.13136 24400.00 20800.00 0.00 0.13263 24600.00 20800.00 0.00 0.13364 24800.00 20800.00 0.00 0.13378 24400.00 21000.00 0.00 0.13500 24600.00 21000.00 0.00 0.13598 24800.00 21000.00 0.00 0.13605 24400.00 21200.00 0.00 0.13741 24600.00 21200.00 0.00 0.13832 24400.00 21400.00 0.00 0.13999 24600.00 21400.00 0.00 0.14084 24200.00 21600.00 0.00 0.14103 24400.00 21600.00 0.00 0.14269 24600.00 21600.00 0.00 0.14346 24200.00 21800.00 0.00 0.14386 24400.00 21800.00 0.00 0.14545 24600.00 21800.00 0.00 0.14614 24200.00 22000.00 0.00 0.14671 24400.00 22000.00 0.00 0.14824 24600.00 22000.00 0.00 0.14885 24200.00 22200.00 0.00 0.14946 24400.00 22200.00 0.00 0.15094 24600.00 22200.00 0.00 0.15148 24200.00 22400.00 0.00 0.15214 24400.00 22400.00 0.00 0.15357 24600.00 22400.00 0.00 0.15403 24200.00 22600.00 0.00 0.15489 24400.00 22600.00 0.00 0.15626 24600.00 22600.00 0.00 0.15663 24200.00 22800.00 0.00 0.15763 24400.00 22800.00 0.00 0.15894 24600.00 22800.00 0.00 0.15921 24200.00 23000.00 0.00 0.16022 24400.00 23000.00 0.00 0.16145 24600.00 23000.00 0.00 0.16158 24200.00 23200.00 0.00 0.16268 24400.00 23200.00 0.00 0.16380 24200.00 23400.00 0.00 0.16490 24400.00 23400.00 0.00 0.16586 24200.00 23600.00 0.00 0.16680 24400.00 23600.00 0.00 0.16755 24200.00 23800.00 0.00 0.16860 24400.00 23800.00 0.00 0.16907 24200.00 24000.00 0.00 0.17023 24400.00 24000.00 0.00 0.17031 24200.00 24200.00 0.00 0.17117 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24193.49 24282.13 28.75 0.17131 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/060.ctf NX, NY, NZ: 360 360 1 MODE : real