CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/055.mrc Output diagnostic file name Micrographs/055.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/055.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2384.000 8308.000 Mean, RMS : 4409.442 222.4475 TITLE 1: emi2mrc: 055.emi Sun Jan 19 14:49:14 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 277.981, Y -464.994, Z -58.220, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03516 15800.00 8000.00 0.00 -0.03483 16000.00 8000.00 0.00 -0.03391 16200.00 8000.00 0.00 -0.03300 16400.00 8000.00 0.00 -0.03213 16600.00 8000.00 0.00 -0.03127 16800.00 8000.00 0.00 -0.03038 17000.00 8000.00 0.00 -0.02940 17200.00 8000.00 0.00 -0.02826 17400.00 8000.00 0.00 -0.02695 17600.00 8000.00 0.00 -0.02551 17800.00 8000.00 0.00 -0.02398 18000.00 8000.00 0.00 -0.02240 18200.00 8000.00 0.00 -0.02080 18400.00 8000.00 0.00 -0.01920 18600.00 8000.00 0.00 -0.01760 18800.00 8000.00 0.00 -0.01599 19000.00 8000.00 0.00 -0.01431 19200.00 8000.00 0.00 -0.01253 19400.00 8000.00 0.00 -0.01063 19600.00 8000.00 0.00 -0.00862 19800.00 8000.00 0.00 -0.00656 20000.00 8000.00 0.00 -0.00452 20200.00 8000.00 0.00 -0.00251 20400.00 8000.00 0.00 -0.00053 20600.00 8000.00 0.00 0.00147 20800.00 8000.00 0.00 0.00353 21000.00 8000.00 0.00 0.00565 21200.00 8000.00 0.00 0.00781 21400.00 8000.00 0.00 0.01000 21600.00 8000.00 0.00 0.01220 21800.00 8000.00 0.00 0.01446 22000.00 8000.00 0.00 0.01681 22200.00 8000.00 0.00 0.01928 22400.00 8000.00 0.00 0.02185 22600.00 8000.00 0.00 0.02453 22800.00 8000.00 0.00 0.02733 23000.00 8000.00 0.00 0.03023 23200.00 8000.00 0.00 0.03325 23400.00 8000.00 0.00 0.03633 23600.00 8000.00 0.00 0.03934 23800.00 8000.00 0.00 0.04209 24000.00 8000.00 0.00 0.04439 24200.00 8000.00 0.00 0.04607 24400.00 8000.00 0.00 0.04708 24600.00 8000.00 0.00 0.04749 24600.00 8200.00 0.00 0.04775 24600.00 8400.00 0.00 0.04799 24600.00 8600.00 0.00 0.04829 24600.00 8800.00 0.00 0.04866 24400.00 9000.00 0.00 0.04870 24600.00 9000.00 0.00 0.04912 24400.00 9200.00 0.00 0.04924 24600.00 9200.00 0.00 0.04968 24400.00 9400.00 0.00 0.04987 24600.00 9400.00 0.00 0.05032 24400.00 9600.00 0.00 0.05056 24600.00 9600.00 0.00 0.05103 24400.00 9800.00 0.00 0.05133 24600.00 9800.00 0.00 0.05181 24400.00 10000.00 0.00 0.05216 24600.00 10000.00 0.00 0.05265 24400.00 10200.00 0.00 0.05297 24600.00 10200.00 0.00 0.05346 24400.00 10400.00 0.00 0.05375 24600.00 10400.00 0.00 0.05422 24400.00 10600.00 0.00 0.05446 24600.00 10600.00 0.00 0.05493 24400.00 10800.00 0.00 0.05512 24600.00 10800.00 0.00 0.05559 24400.00 11000.00 0.00 0.05578 24600.00 11000.00 0.00 0.05624 24400.00 11200.00 0.00 0.05649 24600.00 11200.00 0.00 0.05695 24400.00 11400.00 0.00 0.05722 24600.00 11400.00 0.00 0.05768 24400.00 11600.00 0.00 0.05796 24600.00 11600.00 0.00 0.05842 24400.00 11800.00 0.00 0.05872 24600.00 11800.00 0.00 0.05919 24400.00 12000.00 0.00 0.05951 24600.00 12000.00 0.00 0.05999 24400.00 12200.00 0.00 0.06037 24600.00 12200.00 0.00 0.06085 24400.00 12400.00 0.00 0.06137 24600.00 12400.00 0.00 0.06185 24400.00 12600.00 0.00 0.06250 24600.00 12600.00 0.00 0.06296 24400.00 12800.00 0.00 0.06369 24600.00 12800.00 0.00 0.06414 24400.00 13000.00 0.00 0.06491 24600.00 13000.00 0.00 0.06535 24400.00 13200.00 0.00 0.06615 24600.00 13200.00 0.00 0.06657 24400.00 13400.00 0.00 0.06735 24600.00 13400.00 0.00 0.06776 24400.00 13600.00 0.00 0.06857 24600.00 13600.00 0.00 0.06897 24400.00 13800.00 0.00 0.06984 24600.00 13800.00 0.00 0.07023 24400.00 14000.00 0.00 0.07113 24600.00 14000.00 0.00 0.07151 24400.00 14200.00 0.00 0.07244 24600.00 14200.00 0.00 0.07282 24400.00 14400.00 0.00 0.07382 24600.00 14400.00 0.00 0.07420 24400.00 14600.00 0.00 0.07529 24600.00 14600.00 0.00 0.07567 24200.00 14800.00 0.00 0.07575 24400.00 14800.00 0.00 0.07689 24600.00 14800.00 0.00 0.07729 24200.00 15000.00 0.00 0.07752 24400.00 15000.00 0.00 0.07869 24600.00 15000.00 0.00 0.07911 24200.00 15200.00 0.00 0.07941 24400.00 15200.00 0.00 0.08061 24600.00 15200.00 0.00 0.08104 24200.00 15400.00 0.00 0.08135 24400.00 15400.00 0.00 0.08256 24600.00 15400.00 0.00 0.08299 24200.00 15600.00 0.00 0.08328 24400.00 15600.00 0.00 0.08449 24600.00 15600.00 0.00 0.08489 24200.00 15800.00 0.00 0.08517 24400.00 15800.00 0.00 0.08637 24600.00 15800.00 0.00 0.08673 24200.00 16000.00 0.00 0.08708 24400.00 16000.00 0.00 0.08824 24600.00 16000.00 0.00 0.08855 24200.00 16200.00 0.00 0.08910 24400.00 16200.00 0.00 0.09023 24600.00 16200.00 0.00 0.09049 24200.00 16400.00 0.00 0.09125 24400.00 16400.00 0.00 0.09235 24600.00 16400.00 0.00 0.09256 24200.00 16600.00 0.00 0.09346 24400.00 16600.00 0.00 0.09453 24600.00 16600.00 0.00 0.09469 24200.00 16800.00 0.00 0.09570 24400.00 16800.00 0.00 0.09674 24600.00 16800.00 0.00 0.09687 24200.00 17000.00 0.00 0.09792 24400.00 17000.00 0.00 0.09893 24600.00 17000.00 0.00 0.09903 24200.00 17200.00 0.00 0.10009 24400.00 17200.00 0.00 0.10109 24600.00 17200.00 0.00 0.10117 24200.00 17400.00 0.00 0.10234 24400.00 17400.00 0.00 0.10332 24600.00 17400.00 0.00 0.10339 24200.00 17600.00 0.00 0.10472 24400.00 17600.00 0.00 0.10569 24600.00 17600.00 0.00 0.10574 24200.00 17800.00 0.00 0.10715 24400.00 17800.00 0.00 0.10811 24600.00 17800.00 0.00 0.10814 24200.00 18000.00 0.00 0.10958 24400.00 18000.00 0.00 0.11054 24600.00 18000.00 0.00 0.11055 24200.00 18200.00 0.00 0.11204 24400.00 18200.00 0.00 0.11299 24200.00 18400.00 0.00 0.11448 24400.00 18400.00 0.00 0.11543 24200.00 18600.00 0.00 0.11701 24400.00 18600.00 0.00 0.11795 24200.00 18800.00 0.00 0.11972 24400.00 18800.00 0.00 0.12063 24200.00 19000.00 0.00 0.12249 24400.00 19000.00 0.00 0.12336 24200.00 19200.00 0.00 0.12518 24400.00 19200.00 0.00 0.12600 24200.00 19400.00 0.00 0.12773 24400.00 19400.00 0.00 0.12849 24200.00 19600.00 0.00 0.13009 24400.00 19600.00 0.00 0.13078 24200.00 19800.00 0.00 0.13233 24400.00 19800.00 0.00 0.13296 24200.00 20000.00 0.00 0.13473 24400.00 20000.00 0.00 0.13528 24000.00 20200.00 0.00 0.13556 24200.00 20200.00 0.00 0.13737 24400.00 20200.00 0.00 0.13784 24000.00 20400.00 0.00 0.13838 24200.00 20400.00 0.00 0.14013 24400.00 20400.00 0.00 0.14052 24000.00 20600.00 0.00 0.14123 24200.00 20600.00 0.00 0.14293 24400.00 20600.00 0.00 0.14323 24000.00 20800.00 0.00 0.14403 24200.00 20800.00 0.00 0.14567 24400.00 20800.00 0.00 0.14587 24000.00 21000.00 0.00 0.14666 24200.00 21000.00 0.00 0.14823 24400.00 21000.00 0.00 0.14832 24000.00 21200.00 0.00 0.14929 24200.00 21200.00 0.00 0.15077 24000.00 21400.00 0.00 0.15211 24200.00 21400.00 0.00 0.15348 24000.00 21600.00 0.00 0.15505 24200.00 21600.00 0.00 0.15630 24000.00 21800.00 0.00 0.15801 24200.00 21800.00 0.00 0.15913 24000.00 22000.00 0.00 0.16093 24200.00 22000.00 0.00 0.16192 24000.00 22200.00 0.00 0.16367 24200.00 22200.00 0.00 0.16452 24000.00 22400.00 0.00 0.16630 24200.00 22400.00 0.00 0.16701 24000.00 22600.00 0.00 0.16909 24200.00 22600.00 0.00 0.16960 23800.00 22800.00 0.00 0.16991 24000.00 22800.00 0.00 0.17200 24200.00 22800.00 0.00 0.17227 23800.00 23000.00 0.00 0.17298 24000.00 23000.00 0.00 0.17483 23800.00 23200.00 0.00 0.17590 24000.00 23200.00 0.00 0.17743 23800.00 23400.00 0.00 0.17828 24000.00 23400.00 0.00 0.17944 23800.00 23600.00 0.00 0.17975 24000.00 23600.00 0.00 0.18054 24000.00 23800.00 0.00 0.18091 23800.00 24000.00 15.00 0.18094 23800.00 24000.00 20.00 0.18103 23800.00 24000.00 25.00 0.18110 23800.00 24000.00 30.00 0.18117 23800.00 24000.00 35.00 0.18122 23800.00 24000.00 40.00 0.18125 23800.00 24000.00 45.00 0.18128 23800.00 24000.00 50.00 0.18128 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23731.87 24000.00 53.95 0.18132 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/055.ctf NX, NY, NZ: 360 360 1 MODE : real