CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/038.mrc Output diagnostic file name Micrographs/038.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/038.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2360.000 8252.000 Mean, RMS : 4405.265 226.8089 TITLE 1: emi2mrc: 038.emi Sun Jan 19 14:33:14 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.14%, Obj 88.04%, Dif 73.67% TITLE 3: X 275.209, Y -473.946, Z -58.933, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03328 16400.00 8000.00 0.00 -0.03291 16600.00 8000.00 0.00 -0.03229 16800.00 8000.00 0.00 -0.03164 17000.00 8000.00 0.00 -0.03092 17200.00 8000.00 0.00 -0.03010 17400.00 8000.00 0.00 -0.02921 17600.00 8000.00 0.00 -0.02828 17800.00 8000.00 0.00 -0.02731 18000.00 8000.00 0.00 -0.02625 18200.00 8000.00 0.00 -0.02506 18400.00 8000.00 0.00 -0.02375 18600.00 8000.00 0.00 -0.02240 18800.00 8000.00 0.00 -0.02110 19000.00 8000.00 0.00 -0.01991 19200.00 8000.00 0.00 -0.01880 19400.00 8000.00 0.00 -0.01772 19600.00 8000.00 0.00 -0.01659 19800.00 8000.00 0.00 -0.01538 20000.00 8000.00 0.00 -0.01409 20200.00 8000.00 0.00 -0.01279 20400.00 8000.00 0.00 -0.01151 20600.00 8000.00 0.00 -0.01026 20800.00 8000.00 0.00 -0.00903 21000.00 8000.00 0.00 -0.00780 21200.00 8000.00 0.00 -0.00653 21400.00 8000.00 0.00 -0.00520 21600.00 8000.00 0.00 -0.00377 21800.00 8000.00 0.00 -0.00224 22000.00 8000.00 0.00 -0.00063 22200.00 8000.00 0.00 0.00100 22400.00 8000.00 0.00 0.00257 22600.00 8000.00 0.00 0.00410 22800.00 8000.00 0.00 0.00564 23000.00 8000.00 0.00 0.00733 23200.00 8000.00 0.00 0.00928 23400.00 8000.00 0.00 0.01149 23600.00 8000.00 0.00 0.01387 23800.00 8000.00 0.00 0.01629 24000.00 8000.00 0.00 0.01867 24200.00 8000.00 0.00 0.02101 24400.00 8000.00 0.00 0.02346 24600.00 8000.00 0.00 0.02614 24800.00 8000.00 0.00 0.02907 25000.00 8000.00 0.00 0.03212 25200.00 8000.00 0.00 0.03502 25400.00 8000.00 0.00 0.03748 25600.00 8000.00 0.00 0.03926 25800.00 8000.00 0.00 0.04035 26000.00 8000.00 0.00 0.04082 26200.00 8000.00 0.00 0.04083 26000.00 8200.00 0.00 0.04100 26200.00 8200.00 0.00 0.04101 26000.00 8400.00 0.00 0.04122 26200.00 8400.00 0.00 0.04123 26000.00 8600.00 0.00 0.04157 26200.00 8600.00 0.00 0.04157 25800.00 8800.00 0.00 0.04159 26000.00 8800.00 0.00 0.04206 25800.00 9000.00 0.00 0.04220 26000.00 9000.00 0.00 0.04265 25800.00 9200.00 0.00 0.04287 26000.00 9200.00 0.00 0.04328 25800.00 9400.00 0.00 0.04349 26000.00 9400.00 0.00 0.04387 25800.00 9600.00 0.00 0.04401 26000.00 9600.00 0.00 0.04433 25800.00 9800.00 0.00 0.04443 26000.00 9800.00 0.00 0.04471 25800.00 10000.00 0.00 0.04477 26000.00 10000.00 0.00 0.04501 25800.00 10200.00 0.00 0.04503 26000.00 10200.00 0.00 0.04525 26000.00 10400.00 0.00 0.04545 26000.00 10600.00 0.00 0.04565 25800.00 10800.00 0.00 0.04567 26000.00 10800.00 0.00 0.04587 25800.00 11000.00 0.00 0.04598 26000.00 11000.00 0.00 0.04620 25800.00 11200.00 0.00 0.04646 26000.00 11200.00 0.00 0.04669 25800.00 11400.00 0.00 0.04709 26000.00 11400.00 0.00 0.04734 25800.00 11600.00 0.00 0.04783 26000.00 11600.00 0.00 0.04811 25800.00 11800.00 0.00 0.04866 26000.00 11800.00 0.00 0.04894 25800.00 12000.00 0.00 0.04949 26000.00 12000.00 0.00 0.04977 25800.00 12200.00 0.00 0.05032 26000.00 12200.00 0.00 0.05059 25800.00 12400.00 0.00 0.05116 26000.00 12400.00 0.00 0.05142 25800.00 12600.00 0.00 0.05201 26000.00 12600.00 0.00 0.05225 25800.00 12800.00 0.00 0.05284 26000.00 12800.00 0.00 0.05308 25800.00 13000.00 0.00 0.05366 26000.00 13000.00 0.00 0.05391 25800.00 13200.00 0.00 0.05447 26000.00 13200.00 0.00 0.05473 25800.00 13400.00 0.00 0.05525 26000.00 13400.00 0.00 0.05554 25800.00 13600.00 0.00 0.05609 26000.00 13600.00 0.00 0.05640 25800.00 13800.00 0.00 0.05702 26000.00 13800.00 0.00 0.05734 25800.00 14000.00 0.00 0.05805 26000.00 14000.00 0.00 0.05837 25800.00 14200.00 0.00 0.05918 26000.00 14200.00 0.00 0.05951 25800.00 14400.00 0.00 0.06042 26000.00 14400.00 0.00 0.06074 25800.00 14600.00 0.00 0.06172 26000.00 14600.00 0.00 0.06202 25600.00 14800.00 0.00 0.06207 25800.00 14800.00 0.00 0.06307 26000.00 14800.00 0.00 0.06334 25600.00 15000.00 0.00 0.06351 25800.00 15000.00 0.00 0.06448 26000.00 15000.00 0.00 0.06473 25600.00 15200.00 0.00 0.06495 25800.00 15200.00 0.00 0.06590 26000.00 15200.00 0.00 0.06611 25600.00 15400.00 0.00 0.06636 25800.00 15400.00 0.00 0.06729 26000.00 15400.00 0.00 0.06747 25600.00 15600.00 0.00 0.06776 25800.00 15600.00 0.00 0.06866 26000.00 15600.00 0.00 0.06882 25600.00 15800.00 0.00 0.06915 25800.00 15800.00 0.00 0.07004 26000.00 15800.00 0.00 0.07017 25600.00 16000.00 0.00 0.07059 25800.00 16000.00 0.00 0.07147 26000.00 16000.00 0.00 0.07160 25600.00 16200.00 0.00 0.07218 25800.00 16200.00 0.00 0.07306 26000.00 16200.00 0.00 0.07319 25600.00 16400.00 0.00 0.07392 25800.00 16400.00 0.00 0.07482 26000.00 16400.00 0.00 0.07495 25600.00 16600.00 0.00 0.07574 25800.00 16600.00 0.00 0.07665 26000.00 16600.00 0.00 0.07677 25600.00 16800.00 0.00 0.07758 25800.00 16800.00 0.00 0.07850 26000.00 16800.00 0.00 0.07859 25600.00 17000.00 0.00 0.07936 25800.00 17000.00 0.00 0.08027 26000.00 17000.00 0.00 0.08034 25600.00 17200.00 0.00 0.08106 25800.00 17200.00 0.00 0.08196 26000.00 17200.00 0.00 0.08200 25600.00 17400.00 0.00 0.08278 25800.00 17400.00 0.00 0.08366 25600.00 17600.00 0.00 0.08461 25800.00 17600.00 0.00 0.08547 25600.00 17800.00 0.00 0.08656 25800.00 17800.00 0.00 0.08739 25600.00 18000.00 0.00 0.08863 25800.00 18000.00 0.00 0.08942 25600.00 18200.00 0.00 0.09080 25800.00 18200.00 0.00 0.09155 25600.00 18400.00 0.00 0.09298 25800.00 18400.00 0.00 0.09368 25600.00 18600.00 0.00 0.09518 25800.00 18600.00 0.00 0.09584 25600.00 18800.00 0.00 0.09748 25800.00 18800.00 0.00 0.09810 25400.00 19000.00 0.00 0.09820 25600.00 19000.00 0.00 0.09986 25800.00 19000.00 0.00 0.10043 25400.00 19200.00 0.00 0.10065 25600.00 19200.00 0.00 0.10227 25800.00 19200.00 0.00 0.10281 25400.00 19400.00 0.00 0.10315 25600.00 19400.00 0.00 0.10474 25800.00 19400.00 0.00 0.10526 25400.00 19600.00 0.00 0.10565 25600.00 19600.00 0.00 0.10722 25800.00 19600.00 0.00 0.10772 25400.00 19800.00 0.00 0.10811 25600.00 19800.00 0.00 0.10967 25800.00 19800.00 0.00 0.11015 25400.00 20000.00 0.00 0.11059 25600.00 20000.00 0.00 0.11213 25800.00 20000.00 0.00 0.11258 25400.00 20200.00 0.00 0.11303 25600.00 20200.00 0.00 0.11456 25800.00 20200.00 0.00 0.11499 25400.00 20400.00 0.00 0.11533 25600.00 20400.00 0.00 0.11686 25800.00 20400.00 0.00 0.11727 25400.00 20600.00 0.00 0.11754 25600.00 20600.00 0.00 0.11907 25800.00 20600.00 0.00 0.11948 25400.00 20800.00 0.00 0.11969 25600.00 20800.00 0.00 0.12124 25800.00 20800.00 0.00 0.12166 25400.00 21000.00 0.00 0.12184 25600.00 21000.00 0.00 0.12341 25800.00 21000.00 0.00 0.12382 25400.00 21200.00 0.00 0.12416 25600.00 21200.00 0.00 0.12573 25800.00 21200.00 0.00 0.12612 25400.00 21400.00 0.00 0.12677 25600.00 21400.00 0.00 0.12834 25800.00 21400.00 0.00 0.12869 25400.00 21600.00 0.00 0.12956 25600.00 21600.00 0.00 0.13111 25800.00 21600.00 0.00 0.13140 25400.00 21800.00 0.00 0.13244 25600.00 21800.00 0.00 0.13395 25800.00 21800.00 0.00 0.13417 25400.00 22000.00 0.00 0.13539 25600.00 22000.00 0.00 0.13685 25800.00 22000.00 0.00 0.13699 25400.00 22200.00 0.00 0.13835 25600.00 22200.00 0.00 0.13975 25800.00 22200.00 0.00 0.13980 25400.00 22400.00 0.00 0.14137 25600.00 22400.00 0.00 0.14270 25400.00 22600.00 0.00 0.14465 25600.00 22600.00 0.00 0.14589 25400.00 22800.00 0.00 0.14814 25600.00 22800.00 0.00 0.14927 25400.00 23000.00 0.00 0.15170 25600.00 23000.00 0.00 0.15270 25200.00 23200.00 0.00 0.15280 25400.00 23200.00 0.00 0.15529 25600.00 23200.00 0.00 0.15614 25200.00 23400.00 0.00 0.15640 25400.00 23400.00 0.00 0.15880 25600.00 23400.00 0.00 0.15951 25200.00 23600.00 0.00 0.15994 25400.00 23600.00 0.00 0.16222 25600.00 23600.00 0.00 0.16277 25200.00 23800.00 0.00 0.16368 25400.00 23800.00 0.00 0.16583 25600.00 23800.00 0.00 0.16619 25200.00 24000.00 0.00 0.16776 25400.00 24000.00 0.00 0.16973 25600.00 24000.00 0.00 0.16986 25200.00 24200.00 0.00 0.17199 25400.00 24200.00 0.00 0.17374 25200.00 24400.00 0.00 0.17627 25400.00 24400.00 0.00 0.17774 25200.00 24600.00 0.00 0.18031 25400.00 24600.00 0.00 0.18143 25200.00 24800.00 0.00 0.18357 25400.00 24800.00 0.00 0.18432 25200.00 25000.00 0.00 0.18591 25400.00 25000.00 0.00 0.18625 25200.00 25200.00 0.00 0.18734 25200.00 25400.00 0.00 0.18747 25200.00 25400.00 5.00 0.18757 25200.00 25400.00 10.00 0.18767 25200.00 25400.00 15.00 0.18777 25200.00 25400.00 20.00 0.18785 25200.00 25400.00 25.00 0.18792 25200.00 25400.00 30.00 0.18797 25200.00 25400.00 35.00 0.18800 25200.00 25400.00 40.00 0.18801 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25170.76 25389.29 40.00 0.18804 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/038.ctf NX, NY, NZ: 360 360 1 MODE : real